摘要:

AbstractThe pre‐ and postpartum maternal serum anti‐D concentrations of 28 women with severe Rh(D) immunization who received high‐dose intravenous immunoglobulin treatment has been determined. In all cases, including 1 in which the newborn was D negative, a sharp increment in the anti‐D titer was observed after delivery. The specific immunoglobulin concentration rose to levels ranging from 4.7 to 204.0 μg/ml and, in 20% of the patients, increments of fifty times or greater were observed. Human monoclonal antibodies (hmAb) have been produced from Epstein‐Barr virus‐transformed lymphoblastoid B cell lines derived from 1 of these naturally hyperimmunized patients whose serum contained an anti‐D‐category DVIantibody. Four anti‐D‐secreting cell lines (97.E3.39.214, 44.E4.R1.257, E7.R1.126.83.115 and E11V.117.63; hereafter referred to as 214, 257, 115 and 63) have been established and maintained in continuous culture for periods ranging from several months to 3 years, without loss of antibody production capacity. Antibodies 115 and 214 recognize all Dusamples tested at the same level as the polyclonal positive control. Antibodies 63 and 257 show a significantly lower reaction strength with some of the Dusamples. Studies with D category cells showed that the DVIcategory was recognized only by hmAb 214. The reactivity pattern of this antibody is that of an anti‐epD4, although the reaction strength varied greatly with different DIVacell samples. Results obtained with hmAb 257 and 115 using papain‐treated D category cells suggest that booth react as anti‐epD6/7. Antibody 63 reacts as anti‐epD1, although it shows only weak reactivity with the single sample of DVIIcells tested. A mixture of hmAb 214 and 115 should thus be capable of identifying most weak and partial D erythrocy

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1994.tb00278.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

AbstractA murine monoclonal antibody (mAb i3A; IgG1, kappa light chain) was obtained using human red blood cells as immunogen. The antibody showed Fy6 specificity since it agglutinated all but Fy(a‐b‐)‐untreated red cells and failed to agglutinate chymotrypsin‐treated cells. An erythrocyte membrane protein of 42–46 kD was revealed as the major component recognized by the antibody on immunoblots. The antibody also bound to 92‐ to 95‐ and 200‐kD proteins, tentatively identified as oligomers of the 42‐ to 46‐kD monomeric form. The affinity‐purified Fy6‐active protein was converted to a sharp band of 35 kD after N‐glycanase treatment. The molecule appeared as a slightly broadly band after neuraminidase treatment but was not further altered by O‐glycanase. The i3A mAb bound to 6,000±1,000 receptor sites on either Fy(a‐b+), Fy (a+b+) and Fy(a+b‐) red cells with an affinity constant in the range of 3–6 times 108M‐1. No binding was observed to other blood cells nor to several cells (B, T, myelomonocytic and erythro‐leukemia cell lines). Also, the bulk of i3A‐Fy6 immune complexes could be dissociated from the red cell membrane with as low as 0.2% Triton X‐100, showing that the Fy6‐active glycoprotein is not tightly associated with the membrane skeleton. Our data obtained with a new monoclonal antibody directed to the Fy6 antigen demonstrate that the blood group Duffy‐active component is a red cell‐specific glycoprotei

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1994.tb00279.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

AbstractA discrepancy in duplicate anti‐K1 typing in a parentage case led to the discovery of an unusual K1 blood group antigen. Red blood cells from the propositus (JC) express a rare variant of the K1 antigen that is detectable by only 8 of 72 sera containing anti‐K1. Absorption and elution studies using reactive anti‐K1 confirmed the presence of a K1 antigen. Nonreactive anti‐K1 was not absorbed by or eluted from JC's red blood cells. Red cells from 3 of the propositus's siblings also had the variant K1 antigen. The variant antigen exhibited qualitative as well as quantitative differences as compared to normal K1, and we have named i

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1994.tb00280.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

AbstractThe phenotypic association between the non‐assigned high‐incidence antigen Joaand the Gyacollection antigens Gyaand Hy was investigated by haemagglutination studies, flow cytometric analysis, immune precipitation and immunoblotting experiments. In haemagglutination tests anti‐Joagave the same pattern of reactivity with erythrocytes pre‐treated with pronase, trypsin, α‐chymotrypsin and thiol reducing agents as did anti‐Gyaand anti‐Hy. In addition, similar to that found for anti‐Gyaand anti‐Hy, anti Joaalso showed reduced binding, as determined by haemagglutination and flow cytometric analysis, to erythrocytes from patients with paroxysmal nocturnal haemoglobinuria. Immune precipitates prepared from radio‐iodinated antigen‐positive red cells with anti‐Joa, anti‐Gyaand anti‐Hy gave similar results – a major component of Mr49,000–60,000 (the Gya/Hy‐active glycoprotein) and a second component of Mr85,000–92,000 (this may be a dimer of the Gya/Hy‐active glycoprotein, or a coprecipitated protein). These immune precipitates, when probed with both anti‐Gyaand anti‐Hy under non‐reducing conditions, gave a positive immunoblotting reaction to both the Mr49,000–60,000 and the Mr85,000–92,000 components. These results strongly suggest that the Joaantigen is expressed on the same

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1994.tb00281.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

AbstractRecently, an assay for detection of proviral HIV‐1 DNA in leukocytes became commercially available. This assay (Amplicor HIV‐1 test, Roche Diagnostic Systems) multiplies HIV‐1 DNA up to a detectable level, using the polymerase chain reaction. We studied performance of this assay on 74 samples from HIV‐1‐infected patients and on 41 samples from healthy blood donors. Twice a negative control sample appeared to be erroneously reactive. However, sensitivity and specificity on the patient and donor samples both were 100%. To avoid false‐positive results, we advise to repeat initially reactive samples if no other data confirm HI

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1994.tb00282.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1994.tb00283.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1994.tb00284.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1994.tb00285.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1994.tb00286.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1994.tb00287.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY