摘要:

Abstract.A new δ3 heavy chain disease (δ3–HCD) protein (Emm) is described. A molecular weight of 70,000–80,000 daltons was estimated. Antigenic analysis showed that protein Emm lacks light chains and the CHI domain of heavy chains, whereas the antigenic determinants of the δ3 Fc fragment (CH2 and CH3 domains) as well as those of the δ3 hinge region were present. Since the anomalous protein was temporarily present in serum Emm, a transient triggering of a cell clone which produces the δ3–HCD protein is

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1979.tb04423.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Abstract.HBsAg subtype distribution of 105 positive samples showed 91.4% as ay type. The ay was predominant in different disease conditions, healthy carriers and posttransfusion hepatitis. The prevalence of ay in the Bombay sample is in contrast to the predominance of ad reported from North India. These findings may suggest multifocal infection in different regions of India.

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1979.tb04424.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Abstract.Vasomotor reactions have been reported during infusions of stable plasma protein solutions (SPPS). Possible mechanisms of this phenomenon have been studied using rabbit ear vein preparations. Most samples of SPPS were found inactive but could be activated by proteolytic enzymes. Two factors appear to be responsible: one has kinin‐like properties and the other behaves as kininogen. The latter could be activated by proteases released from both granulocytes and platelets and it is suggested that such activation may occur in clinical use. The bioassay employed in this study provides a basis for modifications in production of SPPS to eliminate these factor

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1979.tb04425.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Abstract.During the course of septicaemia, a group A1patient developed several RBC antigenic modifications. Acquired B antigen, Tk transformation and a partial disappearance of A1receptors associated with an increase of H reactivity were observed.

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1979.tb04426.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Abstract.Utilizing an automated antiglobulin test, we have investigated the presence of the third and fourth components of human complement on normal red blood cells (RBCs). Only negligible amounts of the fourth component, C4, could be detected on either freshly collected or stored RBCs. The fragment C3d of the third component, C3, was detectable on both freshly collected and stored normal RBCs. A product derived from C3 and reacting with anti‐C3c antibody was only barely detectable on freshly collected normal RBCs. During storage of blood at 4°C, increasing quantities of this material were detected on the RBC membrane. Bromelin treatment rendered stored RBCs completely nonreactive with anti‐C3c antibody, whereas only partial loss of reactivity was observed following incubation with heated plasma. In contrast, incubation of EC43 with heated plasma completely abolished their ability to react with anti‐C3c antibody.We suggest that the presence of this C3 fragment on stored RBCs may contribute to the development of ‘preservation

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1979.tb04427.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Abstract.The frequency distribution of reverse passive haemagglutination titres for HBsAg in the sera of 78 positive blood donors found during a 2.5–year period closely follows a lognormal distribution curve. This lognormal distribution suggests that the total population of HBsAg‐positive donors during this period was approximately 84. The modified Hepatest now in use will be expected to detect 82 of these, and to miss only 1 HBsAg‐positive donor in 50,000 screening tests of Manchester blood d

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1979.tb04428.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Abstract.A modified reversed passive hemagglutination test for the detection of hepatitis B surface antigen HBsAg is described. Sera and reagent cells coated with antibody to HBsAg (anti‐HBs) are loaded separately into the rotor of a miniature centrifugal fast analyzer. The rotor is centrifuged briefly to transfer the components into its cuvettes. After mixing, the suspensions are allowed to stand at room temperature for 30 min, following which the rotor is again centrifuged and the absorbance of each cuvette is monitored. Cells suspended in serum containing HBsAg leave the light path more rapidly than cells suspended in sera free of antigen. The magnitude of change in absorbance varies directly with the concentration of the antigen.In 45 sera tested by the conventional V‐plate technique, findings were as follows: 21 positive, 19 false positive and 5 negative. The automated procedure unequivocally differentiated the 21 positives; results for the false positive and negative specimens were identical and clearly distinguishable from the positive results. The automated procedure enhances specificity, offers equivalent sensitivity, and results that are quantitative and object

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1979.tb04429.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Abstract.The technique of proteolytic digestion employing the enzymes trypsin, chymotrypsin, and protease was used to investigate the physical order of subtypic determinants occurring on bovine erythrocytes. In the B system subgroup Y1, Y2, the determinants behaved as if linearly arranged in the same order as predicted from their serological behavior; furthermore, the differences between the two subtypes appeared to be quantitative rather than qualitative. In the E'1, E'2, E'3subgroup, however, the subtypic determinants did not appear to be physically linear, although they are serologically linear.

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1979.tb04430.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Abstract.The property of rhesus alloantibodies to elicit antibody‐dependent, cell‐mediated cytotoxicity (ADCC) against target erythrocytes carrying various Rh genotypes was studied. The killer activity of normal peripheral lymphocytes on human erythrocyte target cells carrying the appropriate antigens elicited by alloantisera was measured by51Cr release at 18 h. There was no correlation between ADCC and antibodies directed to the antigens present on the surface of different genotypes of Rh‐positive red blood cells. The agglutinin titre of different Rh antibodies showed no correlation with the level of ADCC although the degree of cellular cytotoxicity was different with different anti‐D sera. Anti‐C + D + E antibody caused higher ADCC than anti‐C + D and the lowest cytotoxicity was observed with anti‐D and anti‐D + E. This raised the possibility that ADCC was elicited by antibodies directed to other specificities. K cell lysis of human red cells by human peripheral blood lymphocytesin vitrosuggests that a similar mechanism may operatein vitroin the destruction of erythrocytes coated by alloor

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1979.tb04431.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Abstract.Glycerolization of freshly collected human erythrocytes leads to a reduction in ATP levels which return to their original values following deglycerolization. The reduction in ATP levels is largely prevented by pyruvate but not by inosine and glucose, singly or in combination. This implies that the ATP lesion associated with glycerolization reflects mainly a decrease in the NAD/NADH ratio and accordingly a reduced glyceraldehyde–3–phosphate dehydrogenase activity. 2,3–DPG levels were not influenced by glycerolization and deglycerolization.Erythrocytes depleted of ATP in the presence of glycerol can have their ATP levels repleted by either pyruvate or inosine, but require the presence of both compounds for maintenance during a post‐rejuvenation incubation period. This indicates that the prolonged presence of glycerol influences both the NAD/NADH ratio and the carbon‐containing intermediates of glycolysis. Glycerol did not influence ATP repletion of erythrocytes stored at 4°C for 20 days by inosine in combination with pyruvate, but substantially decreased the repletion of their 2,3

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1979.tb04432.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY