摘要:

AbstractA simple process for large‐scale manufacture of ‘high‐purity’ factor VIII is described in detail. A crude concentrate prepared from washed cryo is treated with controlled pore glass (CPG, 500Å pore diameter) in proportion of 20–30 ml of CPG to 1 g input of protein. The slurry is poured into a separation column and the effluent purified concentrate collected. The remaining factor VIII in the void volume is displaced by a wash solution. After passage through a 0.2 μm membrane filter the product is dispensed and lyophilized. Maintaining the operating pH at 6.5–6.7 and adding synthetic amino acids improved the yield and solubility. The current concentrate contains 1 unit of factor VIII per mg protein (10 units mg fibrinogen) with a recovery of 250 units/kg plasma. The CPG stage is non‐destructive, yielding more than 90% of the input factor VIII. In 1980–1983, more than 3times106units have been used in New South Wales, mostly for massive cover in surgical patients. In collaboration with the Commonwealth Serum Laboratories, it is intended to expand production for use in other

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1984.tb00097.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

AbstractThe classic enzyme‐linked antiglobulin test (ELAT) used to detect and quantify the amount of IgG antibodies on red blood cells (RBC) is sensitive to hemolysis and erythrocyte enzymatic activities. We describe a new ELAT by using glucose oxidase (GO) linked to antihuman IgG. The optical density baseline of GO‐ELAT, alkaline phosphatase‐ELAT and peroxidase‐ELAT were, respectively, 0.180, 0.350 and 0.550. This very low baseline of GO‐ELAT was due to the absence of hemolysis (the pH of the GO substrate is 6.5). This technique is ten times more sensitive than the indirect antiglobulin test and detects up to 1 ng/ml of anti‐D alloantibodies. Additional advantages of the technique are (1) there is no intrinsic GO enzyme in RBC, and (2) it is not necessary to

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1984.tb00098.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

AbstractThis paper describes two practical methods for the preservation of pretransfusion patient red blood cells for antigen typing and autoabsorption during a course of transfusion therapy. Blood samples from patients who had serum warm autoantibodies and a positive direct antiglobulin test were collected, the serum frozen, and the red cell aliquots separately preserved by PVP‐methanol or formaldehyde fixation. After storage and recovery, the IgG antibodies were dissociated and the cells used for absorption of the warm autoantibodies. The preserved red cells removed the warm autoantibodies as effectively as fresh red blood cells from the same patient. Preservation of autologous red cells prior to the onset of transfusion therapy provides an extension of the autoabsorption procedure and a simple alternative to differential absorptio

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1984.tb00099.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

AbstractAfter more than 20 years the extended pedigree of the only known kindred carrying the low frequency antigen Ria(Ridley) was reinvestigated. It is established that Riais governed by a gene which is part of or very closely linked to theMNSslocus. Further serological and frequency studies are reported.

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1984.tb00100.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

AbstractOptimum conditions were established for the assay of cold lymphocytotoxic antibodies (cLCTA) in serum from patients with systemic lupus erythematosus (SLE). The optimum temperature of the incubation step with complement was 15°C. For target cells incubated with dextran a significantly higher percentage of damaged cells (p<0.005), a faster rate of cell damage (p<0.001) and a requirement for less complement was observed than for target cells not incubated with dextran. Under optimum conditions in the cytotoxicity assay, 62% of SLE sera damaged more than 60% of the target cells, whereas 73% of the sera from control donors damaged less than 20% of the target cells. In multiple serum samples obtained during follow‐up of 30 patients with SLE, the serum cytotoxic activity or titer of cLCTA fluctuated and did not relate to clinical symptoms of disease activity. In sera obtained during active disease the cytotoxic activity was related to the anti‐dsDNA antibody level and with levels of circulating immune complexes measured by the Raji cell assay and the indirect granulocyte phagocytosis test. We found no restricted reactivity of cLCTA against phenotypically distinct T‐lymphocyte subpopulations with different immunoregulatory functions. The reactivity pattern of cLCTA towards the various T‐lymphocyte subpopulations was not related to disease activity. We conclude that cLCTA in patients with SLE represent an epiphenomenon, being the result of a hyperreactive B‐cell system rather than the cause of t

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1984.tb00101.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

AbstractDantu, a previously undescribed low‐incidence red cell antigen, is inherited as a Mendelian dominant character. The Dantu antigen is associated with very weak s antigen, protease resistant N antigen and either very weak or no U antigen. Two of the propositi had previously been shown to have an unusual hybrid MNSs sialoglycoprotein, and it is probably this which carries these unusual N, s and U antigens as well as the Dantu antigen. A study of the family of one propositus suggests, by conventional genetics, thatDantuis not controlled by theMNSslocus; a possible explanation is given. Several examples of anti‐Dantu are known, one was found to cause a positive direct antiglobulin reaction on neonatal red ce

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1984.tb00102.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1984.tb00103.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1984.tb00104.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1984.tb00105.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1984.tb00106.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY