摘要:

Abstract.Attempts to produce anti‐D in rhesus‐negative men using reconstituted frozen rhesus‐positive cells were unsuccessful. The use of cells coated with IgM anti‐D for immunisation stimulates anti‐D production, and a comparison between two groups, one having cells coated with IgM anti‐D, and the other having identical cells without coating suggests that there is an enhancement of the immune response when IgM‐coated c

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1977.tb00628.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

Abstract.This report describes a case of persistent polyagglutinability restricted to the red blood cells, and associated with hemolylic anemia in a 20‐year‐old male. Anemia was first noted at the age of 5 months and has persisted up to the present time with normal intervals. His red blood cells were weakly agglutinated by almost all adult sera. No autoagglutination was noted and the direct antihuman globulin test was negative. Polyagglutinability of his red blood cells was clearly distinguishable from T, Tn and Tk. No reaction was noted withDolichos biflorusand peanut extracts. The cells differed from normal erythrocytes in their positive reactions with various snail agglutinins and their weak reaction with a range of anti‐H reagents. There was normal aggregation by Polybrene. MN determinants were normally expressed. The symbol ‘VA’ is proposed for this type of polyaggl

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1977.tb00629.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

Abstract.With help of immunofluorescence, best with anti‐AHp fromHelix pomatia, a stippled structure could be demonstrated on the patient's red blood cells. Thus an “A‐like” receptor could be detected on the erythrocyte membrane of this group O patient. The reactive antigen was proved not to be a crypt antigen exposed by the action of neuraminidase. The same stippled fluorescence with anti‐AHp was observed on the red blood cells of a patient suffering from hemolytic anemia induced by influenza A, virus. In this case this virus was shown not to be responsible for polyagglutination. No virus or microorganism could be isolated from the patient's blood. Also by immunofluorescence the weak expression of the H antigen could be demonstrated with an extract ofEvonymus europaeus.Electron microscopy of erythrocytes was normal. The neuraminic acid content and the electrophoretic mobility were found to be decreased to a minor degree. No distinct cell populations could be

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1977.tb00630.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

Abstract.In a series of 726 random Caucasian adults tested with anti‐Coaand anti‐Coballele frequencies wereCoa<0.9470,Cob0.0530. The distribution of Colton phenotypes in 205 children of 57 selected families was in accordance with expectation. A linkage analysis ofCoand 26 other loci indicates thatCois not closely linked toAcP1, ABO,AK1, Ch, Do, GLO, Gm, GPT, HLA, Hp, K, Lu, MN, PGM1orRhand does not lie betweenPGDandPGM1on chromosom

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1977.tb00631.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

Abstract.A ‘new’ Lutheran‐related antibody, named anti‐Lu14, reacts with approximately 2.4% of random bloods. Red cells of the rare Lu:‐8 phenotype are Lu:14. The data indicate, with a high probability, that the Lu 14 antigen is a product of an allele ofLu8and thatLu14andLu8comprise a third pair of alleles at the Lutheran locus. Red cells of the original Sw (a+) propositus are Lu:14. By coincidence, he has inherited two low‐incidence genes. This observation may explain the discrepancy in different families concerning a possible relationship betweenSwaand Lutheran. Pedigree information now suggests thatSwais not a Lu

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1977.tb00632.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

Abstract.This paper gives details of a family in which there are numerous examples of the heterozygousKostate. The Study was initiated by the presence of anti‐Kpbin the serum of the propositus. Reactions with anti‐k, anti‐Ku, anti‐Jsband anti‐K13 confirm that theKogene intranswithKparesults in weaker expression of these Kell antigens. One example of anti‐k showed some evidence of dosage effect with kKpb/K° cells, but these results were not confirmed by quantita

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1977.tb00633.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

Abstract.A total of 481 serum samples was collected from asymptomatic carriers of H B,Ag as well as some patients with acute and chronic hepatitis, type B. The sera were obtained mainly from blood banks in the following countries: Poland, Hungary, Romania, Bulgaria, Yugoslavia, and the European part of the Soviet Union. Asymptomatic carriers of HBsAg from Poland and Hungary showed a preponderance ofadwsubtype overuywsubtype (Poland ‐80.7%adw, Hungary ‐72.2%adw).Carriers from the remaining countries showed the reverse situation: Bulgaria ‐71.5%ayw, Yugoslavia ‐79.0%ayw, Romania ‐82.8%ayw, Soviet Union ‐87.5%ayw.No individuals were found among these indigenous populations to have theadrorayrsubtypes but two carriers appeared to have the unusual

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1977.tb00634.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

Abstract.23 patients with hemophilia B have been investigated by means of several immunological methods. 16 patients (69.9%) had no detectable factor XI antigen. Five had a normal factor IX antigen and the electrophoretic mobility of this abnormal factor IX was similar to that of its normal counterpart. One of these five patients had hemophilia BW, since ox brain thromboplastin clotting time was severely prolonged. The remaining two patients had reduced or decreased factor IX antigen. Several patients showed a slight prolongation of ox brain thromboplastin time due to an associated slight factor VII deficiency. On the basis of these results, a tentative classification of hemophilia B into five variants is proposed, namely: hemophilia B, or with no factor IX antigen; hemophilia Bt, or with normal factor IX antigen; hemophilia BRA, or with reduced factor IX antigen; hemophilia BM, or with normal factor TX antigen and severely prolonged ox brain thromboplastin; hemophilia B, usually B‐, with associated mild factor VII defect. A complete evaluation of the hemophilia B patients is feasible only by means of a battery of tests, namely: factor TX activity assay, factor IX antigen determination, ox brain thromboplastin clotting time, factor VII activity assa

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1977.tb00635.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

Abstract.Various lots of human serum albumin, prepared by the Cohn alcohol procedure, were assayed for polymer distribution after lyophilization or acetone drying of the albumin paste. Sodium dodecyl sulfate polyacrylamide gel electrophoresis revealed that powders obtained by acetone drying of the pastes contained significantly higher amounts of monomeric albumin.

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1977.tb00636.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

Abstract.A method is described for albumin dimer determination. The method is based upon an electrophoretic separation of albumin dimer from the monomer in starch gel with a subsequent immunochemical diffusion and precipitation of the albumin components in agarose gel containing antibodies. The precipitation zones are measured and compared with those of albumin monomer standards. A factor for converting monomer to dimer has furthermore been established.

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1977.tb00637.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY