摘要:

Abstract.A single‐step method is described for the isolation of a highly purified antithrombin III (AT III) concentrate at a recovery of over 30% using affinity chromatography on heparin‐Sepharose (HS). The polyethylene glycol precipitation step frequently employed in the preparation of AT III concentrates for clinical use has been eliminated and purification is accomplished entirely by optimizing the salt concentration in the HS washing buffer to enhance the desorption of impurities prior to elution of AT III. Pasteurization of the AT III concentrate in the presence of 0.5Msodium citrate to minimize the risk of hepatitis decreases the recovery by about 20% and induces changes in the patterns obtained by polyacrylamide gel electrophoresis and by crossed immunoelectrophoresis in heparinized agarose

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1984.tb03875.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Abstract.In vitro platelet function during 7 days of storage at room temperature was studied in a conventional polyvinylchloride plastic bag F 76 and in a new plastic bag F 702 which contained as plasticizer a phtalateester analogue. This new material has increased permeability to oxygen and carbon dioxide, and therefore a pH decrease does not occur during 7 days of platelet storage. The decrease of plasma glucose concentration and the increase of plasma lactate in the new bag is less than in the standard plastic currently in use. In vitro platelet function measured as hypotonic shock reaction, aggregation response to ADP and collagen and14C‐serotonine uptake was better than that found with the standard material. The data indicate that the use of the new platelet storage container F702 will permit satisfactory storage for at least 5 days at 22 °C. It is suggested that it will even improve the quality, as measured by in vitro tests, of platelets stored up to 72 h compared to the standard plast

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1984.tb03876.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Abstract.Using classical typing antisera, previous experiments have failed to demonstrate IgG3 in partially reduced and alkylated preparations of human IgG intended for intravenous application (IGIV). To establish that IgG3 is actually present in such preparations, we designed an enzyme‐linked immunosorbent assay (ELISA) using monoclonal antibodies as solid‐phase reagents and protein A‐purified IgG3 as antigen. Three different samples of reduced and alkylated antigen were used: (1) IgG3 isolated from a ready‐for‐infusion IGIV; (2) IgG3 which was purified from an intramuscular (Cohn fraction II) IgG solution before being subjected to a mild reduction and alkylation procedure, and (3) completely reduced and alkylated IgG3. The reduction and alkylation procedure did not affect the solubility of IgG3, indicating that IGIV prepared in this manner should contain normal quantities of IgG3. In the ELISA, solid‐phase monoclonals which were cross‐reactive with multiple IgG subclasses clearly reacted with reduced and alkylated IgG3. Furthermore, there was no substantial difference between the quantities of modified and native antigen required for 50% maximal ELISA signal. In contrast, solid‐phase monoclonals with IgG3‐restricted specificity did not recognize reduced and alkylated material. These results indicate that IGIV prepared by reduction and alkylation has a normal IgG3 content and confirm that some IgG3‐specific determinants are altered by the mod

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1984.tb03877.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Abstract.The immunoglobulin classes of platelet alloantibodies were studied using monospecific antisera in a radiolabeled technique. 14 of 28 consecutively studied multiply transfused oncology patients demonstrated platelet antibodies. These 14 patients were also refractory to random donor platelets. The immunoglobulin classes of these platelet antibodies were found to be: IgG alone 4, IgM alone 4, IgG and IgM 5, IgM plus IgA 1. The results in this study suggest that using monospecific antisera other than anti‐IgG alone, one can detect additional antibodies involved in the immune destruction of platelet

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1984.tb03878.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Abstract.A hybridoma (ES‐15) was obtained by fusing the NS‐1 cell line with spleen cells from a mouse immunised with soluble blood group A2substance. The cloned hybridoma culture supernatant was shown to contain an IgM class antibody which strongly agglutinates group A cells and weakly agglutinates group B cells. The serological specificity of this antibody is described as anti‐A,(B) in this report. The abilities of unconcentrated monoclonal anti‐A,(B), a commercial human polyclonal anti‐A,B (group O serum) and a commercial monoclonal anti‐A reagent to detect 15 examples of Axcells were compared by both slide and tube techniques. Using a slide technique monoclonal anti‐A,(B) agglutinated 14 examples of Axcells, human anti‐A,B 2 examples, while monoclonal anti‐A failed to detect any of the Axcells tested. Similar differences in the reactivity of the three antibodies were observed using a tube technique. Data are also presented which show that a 1:1 (v/v) mixture of monoclonal anti‐A,(B) with a monoclonal anti‐B reagent is an effective replacement for human anti‐A,B in

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1984.tb03879.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1984.tb03880.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1984.tb03881.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1984.tb03882.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1984.tb03883.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1984.tb03884.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY