摘要:

Abstract.The preparation of a specific anti‐T cell serum, applicable in the indirect immuno‐fluorescence technique on cell suspensions, smears of peripheral blood and bone marrow and on tissue sections is described. Rabbits were immunized with thymocytes; after removal of antibodies against species‐specific antigens and antigens common to leucocytes by absorptions with red cells and granulocytes, specificity for thymocytes was obtained by repeated absorptions with CLL cells, used as B cell equivalents. Subsequently, the IgG fraction of the absorbed antiserum was isolated. This antibody preparation was tested with various types of blood cells as well as with cell suspensions depleted or enriched in T cells. For the study of tissue sections it had to be absorbed with liver powder. When studying lymphatic tissue it was found to stain only thymus‐dependent areas in the specimens

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1977.tb04471.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

Abstract.An antibody to an antigen on IgG was found in the serum of a healthy American Negro, whose phenotype is Gm (1, 13, 15, 17). (When tested for Gm [1, 2, 3, 5, 6, 13, 15, 16, 17, 21].) Subsequent tests of serum samples from US blacks and whites, from Japanese, Ainu, San (Bushmen), Negros, Asiatic Indians and Jews from Cochin India demonstrated that the antibody detects an antigen that is usually present in a haplotype when Gm (15) is absent from it. This antigen had been identified using an antibody produced in a baboon. Tests of isolated myeloma proteins and of Fc and Fab fragments of IgG confirmed that the antigen [Gm (26) or Gm (u)—originally Gm (Pa)]is carried by the Fc portion of the γ3‐c

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1977.tb04472.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

Abstract.The pattern of HLA segregation was analyzed in 10 sibships of haemophilia A patients, each of which included 1 or 2 haemophilic brothers with an antibody to factor VIII. The observed HLA distribution is suggestive of a linkage between the major histocompatibility complex and a gene responsible for immune response to isologous factor VIII.

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1977.tb04473.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

Abstract.Two sisters who developed nodular sclerosis Hodgkin's disease (H.D., N.S.) within 3 months of their 25th birthdays were HLA and MLC identical. All family members possessed the HLA‐B7 antigen. The propositi, a third HLA/MLC identical sister, and the mother, shared the maternal B7 and lacked Dw2. The father and a fourth sibling with the paternal B7 were heterozygous for Dw2. In six unrelated patients with H.D., N.S., the presence of B7 and Dw2 correlated precisely. In 69 consecutive patients with H.D., N.S., the frequencies of 23 HLA antigens did not differ significantly from the frequencies in 245 control subject

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1977.tb04474.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

Abstract.The HLA types of two families with hereditary spherocytosis (HS) suggest that theHSgene(s) may be linked with theHLAlocus. If substantiated by further family studies, this linkage would placeHSon chromosome 6.; Accepted: February 14, 1977

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1977.tb04475.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

Abstract.Human IgM and IgG antibodies against blood group antigens (A, B, D, C, c, E, e, Fya, K), autoantibodies and mouse IgM and IgG antibodies against sheep erythrocytes have been eluted from intact human and sheep red cells by glycine‐HC1 buffer, pH 3.0. The yield of human antibodies was higher with acid than with heat and ether elution, and the contamination of hemoglobin in the eluate was negligible. The acid elution method is very simple and rapid and, therefore, highly suitable for experimental as well as routine immuno‐hematological w

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1977.tb04476.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

Abstract.The so‐called 'cisAB' blood group is accounted for by proposing that 'normal' anti‐A and anti‐B reagents are cross‐reacting withpartially overlapping reaction ranges. Hence, they are labelled anti‐AX and anti‐BX, respectively. Some consequences of a complex‐simple model where'cisAB' is accordingly produced by a simple (mono‐factorial) antigen X (produced by a simple geneXat the ABO‐locus) are

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1977.tb04477.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

Abstract.In a case of disputed paternity genetic incompatibility was observed in the ABO blood group system between mother (O) and child (AB). From biostatistical evaluation of 21 genetic markers, including HLA phenotypes, a high value of probability for paternity, maternity and parentage was found between the child, the child's mother, the accused man and his mother. Substitution of the newborn was thereby excluded. The serostatistical evaluation of maternity and paternity could be supported by anthropological opinion. In serological investigations of the ABH system the A and B antigens of the 'A2B' cells were found to be abnormal in the child, the child's father, and the father's mother: the A was weaker than normal A1but stronger than normal A2; the B was found to be abnormal which in addition was documented through occurrence of irregular anti‐B antibodies in the child. In comparison to normal controls (A1, A2, B, A1B, A2B) diminished activity of α‐D‐N‐acetylgalactosamine and α‐D‐galactosyltransferase were observed in the three 'A2B' propositi. These facts confirmed the existence of a cis‐AB gene in the Lam. family which the child inherited from her father and the child's father from his mother. Assuming a population frequency of 1.1 times 10−5for the cis‐AB gene, the probability for paternity was calculated from all genetic markers to

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1977.tb04478.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

Abstract.LW‐negative (LW4) red blood cells and anti‐LW antibody were discovered in the blood of a 50‐year‐old white male with Hodgkin's disease and blood type O. Compatible red blood cells for transfusion could not be obtained because available LW‐negative cells were either type A, LW4, or type O, LW3. Following cytotoxic therapy for his basic disease, the anti‐LW disappeared and his red blood cells developed normal reactions with a

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1977.tb04479.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

Abstract.The specificity anti‐Gd of human cold autoagglutinins is characterized using untreated and enzyme‐treated human red blood cells. Gd determinants of human RBC are resistant to proteases, but are inactivated by neuraminidase (RDE). In contrast, I/i determinants are not inactivated by proteases or RDE, while Pr1–3determinants are inactivated by proteases and RDE, and Pradeterminants are resistant to RDE, but are inactivated by prot

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1977.tb04480.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY