ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1981.tb00701.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Abstract.Four methods were investigated to determine their suitability as platelet compatibility procedures: leukoagglutination, lymphocytotoxicity, platelet suspension immunofluorescence and platelet enzyme‐linked immunosorbant assay. None of the tests were found to reliably predict the 24‐hour‐posttransfusion platelet increment in 8 refractory thrombocytopenic patients judged refractory to random donor platelet th

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1981.tb00702.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Abstract.Plateletpheresis units collected using the Haemonetics Model 30 or the IBM 2997 cell separator were stored for 24h at 22 ° C. Autologous recovery and life span measurements averaged 64 ± 13% (1 SD) and 8.9 ± 1.3 (1SD) days. The hemostatic effectiveness of these preparations were satisfactory; the corrected increment 4–6 h and 18–24 posttransfusion was 20,400 and 13,400/μ1/m2, respectively, and the bleeding time showed improvement in each patient. There was no significant difference in these parameters between collection

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1981.tb00703.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Abstract.The intracellular distribution of radioactivity was studied in normal and sickle erythrocytes labeled with sodium51Cr chromate. Both types of cells had a higher fraction of51Cr bound to hemoglobin when labeled in the presence of ACD at a pH of 7.09 than when labeled in the presence of CPD at a pH of 5.96. Citrate at a pH of 5.96 or less entered the red blood cells and decreased the51Cr binding hemoglobin. Binding of51Cr to hemoglobin within the red cells was also reduced when the ATP and DPG levels in the red blood cells were elevated. Studies with hemoglobin solution showed that51Cr binding to hemoglobin was influenced by 2,3‐DPG, ATP and citrat

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1981.tb00704.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Abstract.Three anti‐Rh sera useful for the EAhumtest were found (one comparable to Ri serum). It was shown that the usefulness of anti‐Rh sera for the EAhumtest depends on the amount of anti‐Rh antibodies absorbed onto red cells demonstrated in the manual antiglobdin test. It does not depend on the quality of antibodies and the ability of complement bi

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1981.tb00705.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Abstract.This report describes the recovery of highly purified immune‐serum globulin (ISG) from the 42% ethanol precipitate fraction (42% P) generated during the first step of albumin purification. The method consists of three purification steps: (1) reprecipitation of the 42% P at 20% ethanol, pH 7.2; (2) solubilization of ISG at 17% ethanol, pH 5.2, and (3) isoelectric precipitation of ISG at 25% ethanol, pH 7.2. ISG prepared by this method was homogeneous when subjected to immunoelectrophoresis and polyacrylamide gel electrophoresis, and gave a yield equivalent to 4.7 g/l plasma, corresponding to a final recovery of over 48

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1981.tb00706.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Abstract.Microaggregate‐poor whole blood is prepared by collecting blood in ordinary double or triple plastic bag systems, separating cell‐poor plasma and buffy coat from the red cells but without disconnecting the plasma bag and the red cell bag from each other. The double unit is stored at refrigerator temperature. After 10 days' storage, the tube connecting the two parts of the double unit can be sealed upon which further storage is done separately. In this way all whole blood units in stock will have both a low content of microaggregates and a normal or nearly normal affinity for oxy

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1981.tb00707.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1981.tb00708.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1981.tb00709.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1981.tb00710.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY