|
1. |
Removal of Leukocytes from Blood by Fibre Filtration: A Comparison Study on the Performance of Two Commercially Available Filters |
|
Vox Sanguinis,
Volume 42,
Issue 6,
1982,
Page 281-288
H. W. Reesink,
H. Veldman,
H.J. Henrichs,
H.K. Prins,
J.A. Loos,
Preview
|
PDF (442KB)
|
|
摘要:
Abstract: Two different kinds of filters suitable for the almost complete removal of leukocytes from blood‐cell concentrates were tested. The maximal retention of filter I was 1.9‐3.4×109leukocytes per filter, whereas filter II could retain 3.6–7.8×109leukocytes per filter before the leukocyte concentration in the filtrate passed the level of 500 leukocytes/μl. The leukocytes, once absorbed by the fibre material, could be released by washing the filters I, whereas the leukocytes were retained by the material of the filters II. No detectable particles were released after the first 100 ml of filtrate during the washing procedure of either kind of filters. From more than 20% of the filters I, more than 500 pg/ml of endotoxin could be released during the prewashing, whereas none of the filters II was contaminated with endotoxin. The filter II released acetic acid which could be completely removed during the prewashing with 250 ml of saline solution. Operation according to the prescribed conditions of 25 filters of both kinds revealed that the residual leukocyte content in the filtrate was more than 0.25 ×109leukocytes in 8 out of 25 of the filtrates when filters I were used, whereas with all filters II, this content remained lower than 0.1×109leukocytes per filtrate. It was concluded that only filter II has sufficient capacity to guarantee the removal of 97% of all leukocytes and 90% of the thrombocytes present in 500 ml of fresh h
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1982.tb01100.x
出版商:Blackwell Publishing Ltd
年代:1982
数据来源: WILEY
|
2. |
Production of Direct Agglutinins by the Combination of Incomplete Antibodies to Protein A‐BearingStaphylococcus aureusCells |
|
Vox Sanguinis,
Volume 42,
Issue 6,
1982,
Page 289-294
I.J. Deary,
S. Moore,
D.S. Pepper,
Preview
|
PDF (355KB)
|
|
摘要:
Abstract: A novel method is described for the production of saline‐reacting antibodies from ‘incomplete’ blood grouping sera. IgG anti‐D is combined to protein A‐bearingStaphylococcus aureus(SA), forming a polyvalent anti‐D assembly. The SA‐anti‐D has a high titre in saline and is stable on 4°C storage and lyophilisation. The material was successful in Rh(D) typing cells sensitised in vitro with various antibodies. Saline‐reacting SA‐anti‐Kell and SA‐an
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1982.tb01101.x
出版商:Blackwell Publishing Ltd
年代:1982
数据来源: WILEY
|
3. |
Ulex Seed Extracts: Lymphocyte Growth Inhibition and the Anti‐H Hemagglutinins1 |
|
Vox Sanguinis,
Volume 42,
Issue 6,
1982,
Page 295-303
Bernard Pirofsky,
Kristy R. Ingebo,
Anne V. August,
Stephen H. Hefeneider,
Preview
|
PDF (589KB)
|
|
摘要:
Abstract: Extracts produced from the seeds ofUlex europaeusare commonly used for their ability to react with the H basic substance present on erythrocytes and secreted in body fluids. Such extracts were found to also contain a potent inhibitor of human and murine lymphocyte growth. Inhibition of growth does not result from cytotoxicity and is easily reversible. Ulex seed extract (USE) solutions were modified in various ways to produce reagents in which the anti‐H hemagglutinins were either retained or removed. The fractionated solutions were then analyzed for hemagglutination and lymphocyte growth‐inhibiting activity. Such studies clearly indicated that these two biological functions resulted from the action of different materials. The lymphocyte growth inhibitor is not a glycoprotein lectin. It does not mediate its effect through the H basic substance and is a heat‐stable, small molecule. The data suggest that plant seed extracts employed for their lectin content may contain an additional class of biologically active agents potentially useful i
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1982.tb01102.x
出版商:Blackwell Publishing Ltd
年代:1982
数据来源: WILEY
|
4. |
Immunological Monitoring of a Patient with Guillain‐Barré Syndrome Successfully Treated with Plasma Exchange |
|
Vox Sanguinis,
Volume 42,
Issue 6,
1982,
Page 304-307
G. Fiorini,
G. Bigi,
M. L. Paracchini,
C. Marinig,
A. Gibelli,
Preview
|
PDF (244KB)
|
|
摘要:
Abstract:A patient with Guillain‐Barré syndrome was successfully treated by plasma exchange (without any other therapy), and the relationship between changes in his peripheral blood lymphocyte subpopulations and the clinical improvement induced by plasma‐pheresis was studied. At the recovery, the most interesting findings were an increase in T suppressor lymphocytes, and in Fc receptor‐bearing lymphocytes (above normal values), together with a decrease in B lymphocytes. In this patient, plasma exchange not only removed toxic humoral factors, but also restored a normal balance among peripheral lymph
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1982.tb01103.x
出版商:Blackwell Publishing Ltd
年代:1982
数据来源: WILEY
|
5. |
The Serology of SdaEffects of Transfusion and Pregnancy |
|
Vox Sanguinis,
Volume 42,
Issue 6,
1982,
Page 308-312
Steven Spitalnik,
Mary T. Cox,
Janice Spennacchio,
Rita Guenther,
Neil Blumberg,
Preview
|
PDF (307KB)
|
|
摘要:
Abstract:Pre‐ and posttransfusion antibody titers were performed on 6 patients with anti‐Sdatransfused with incompatible blood. In 3 of these patients a significant rise in IgG antibody titer was found. The data suggest that in occasional patients the Sdaantigen does evoke a secondary immune response. We evaluated 245 pregnant women for the presence of Sdaand found that 30% were Sd(a‐). This incidence was significantly higher than that found in normal blood donors (4%), but was lower than that described in previous reports. We found that 22% of pregnant women in their first trimester were Sd(a‐) whereas at term 36% were Sd(a‐). These significantly different incidences of antigen positivity suggest decreased antigen expression with progressing pregnancy, as seen in the Lewis system. No difference was found in the incidence of anti‐Sdabetween pregnant women, either during their first trimester or at term, and no
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1982.tb01104.x
出版商:Blackwell Publishing Ltd
年代:1982
数据来源: WILEY
|
6. |
Two New Slow‐Moving Variants of Human Serum Albumin |
|
Vox Sanguinis,
Volume 42,
Issue 6,
1982,
Page 313-317
Hiroaki Nishimukai,
Yoshio Kera,
Yuji Sano,
Kiyoshi Sakata,
Hajime Kitamura,
Kosuke Tsukamoto,
Kichihei Yamasawa,
Preview
|
PDF (309KB)
|
|
摘要:
Abstract:Two new slow‐moving variants of human serum albumin were found in members of two unrelated Japanese families. These variants, different from each other and from the slow‐moving variants described previously, were designated albumin ‘Okinoerabu’ and albumin ‘Akasaka’. The mode of inheritance of each variant was consistent with an autosomal codominant trait. A very rare type, homozygote for a variant, albumin ‘Okinoerabu’, was also detected. The segregation data of albumin and Gc showed no discrepancy from the postulation of a linkage between
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1982.tb01105.x
出版商:Blackwell Publishing Ltd
年代:1982
数据来源: WILEY
|
7. |
Febrile Transfusion Reaction: What Blood Component Should Be Given Next? |
|
Vox Sanguinis,
Volume 42,
Issue 6,
1982,
Page 318-321
Jay E. Menitove,
Margaret C. McElligott,
Richard H. Aster,
Preview
|
PDF (258KB)
|
|
摘要:
Abstract:Reports of febrile, nonhemolytic transfusion reactions (FNHTR) occurring at hospitals served by a regional blood center supplying 99,658 units of blood during 1980 were analyzed to determine if leukocyte‐poor red blood cells prepared by the inverted centrifu‐gation technique (LP RBCs) were adequate to prevent subsequent reactions. FNHTR occurred following 0.5% of units transfused. The records of transfusions given to patients who had a FNHTR were reviewed in a subgroup of hospitals. Of 253 such patients, 161 received subsequent transfusions. 140 received red cells or LP RBCs without a reaction. The remaining 21 had a second reaction following transfusion of packed red cells. 12 of the 21 received further red cell transfusions. Only one experienced a third febrile reaction after receiving LP RBCs. We conclude that LP RBCs are adequate to prevent recurrence of FNHTR and question the need for costly saline‐washed, leukocyte‐poor red blood cells for this
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1982.tb01106.x
出版商:Blackwell Publishing Ltd
年代:1982
数据来源: WILEY
|
8. |
Summary of the Third Meeting of the ISBT Working Party on Automation and Data Processing New York, USA, October 27–30, 1981 |
|
Vox Sanguinis,
Volume 42,
Issue 6,
1982,
Page 322-324
Preview
|
PDF (177KB)
|
|
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1982.tb01107.x
出版商:Blackwell Publishing Ltd
年代:1982
数据来源: WILEY
|
9. |
The Immune System: A Tribute to Niels Kaj Jerne |
|
Vox Sanguinis,
Volume 42,
Issue 6,
1982,
Page 325-325
C. M. Steinberg,
I. Lefkovits,
Preview
|
PDF (66KB)
|
|
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1982.tb01108.x
出版商:Blackwell Publishing Ltd
年代:1982
数据来源: WILEY
|
|