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1. |
Cryopreservation of Human Platelets Using 6% Dimethyl Sulfoxide and Storage at ‐80° |
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Vox Sanguinis,
Volume 49,
Issue 4,
1985,
Page 245-258
A.J. Melaragno,
R. Carciero,
H. Feingold,
L. Talarico,
L. Weintraub,
C. R. Valeri,
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摘要:
AbstractPlatelet studies were done in healthy male volunteers and in thrombocytopenic patients. Some of the platelets used in the study were isolated by mechanical apheresis using either the Haemonetics blood processor 30, the IBM blood processor 2997 or the Fenwal CS‐3000 blood processor before freezing. Other platelets were isolated from individual units of whole blood and pooled before freezing. The platelets were frozen with a 6% cryoprotectant (DMSO) in a polyvinylchloride (PVC) plastic bag or a polyolefin plastic bag at ‐80°C in a mechanical freezer and stored for as long as 3 years. Some of the frozen platelets were transported in dry ice in polystyrene foam containers to determine whether they would be adversely affected by such treatment. Platelet recovery after freezing, thawing and washing was about 75%. In the healthy male volunteers, in vivo recovery of autologous platelets 1–2 h after transfusion was about 33%, and the life span was about 8 days. In the thrombocytopenic patients, in vivo recovery values were 50% of those from fresh platelets. The transfusion of previously frozen washed platelets reduced clinical bleeding in the thrombocytopenic patients with bleeding. There was no evidence of quality deterioration in platelets after storage at ‐80°C for at least 2 years, as determined from in vitro recovery and in vivo survival values, nor was there any adverse effect as a result of shipment of the frozen platelets in dry ice in polystyrene foam containers from one facility t
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1985.tb01119.x
出版商:Blackwell Publishing Ltd
年代:1985
数据来源: WILEY
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2. |
Macroaggregate Formation in Optimal Additive Red Cells |
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Vox Sanguinis,
Volume 49,
Issue 4,
1985,
Page 259-266
M. Robertson,
F. E. Boulton,
R. Doughty,
J. R. MacLennan,
A. Collins,
D. B. L. McClelland,
C. V. Prowse,
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摘要:
AbstractThe formation of macroaggregates weighing up to 9 g was observed in optimal additive red cells. Such aggregates, with a mean wet weight of 3 g, formed progressively during storage and were present in up to 85% of units. They were composed of leucocyte and platelet debris, together with some fibrin. Macroaggregate formation was halved by less stringent centrifugation during preparation and was reduced by use of an optimal additive system in which the additive solution contained citrate. Extra mixing during transfer of the additive solution only delayed aggregate formation. Partial leucocyte depletion or addition of 200,000 KIU of the enzyme inhibitor aprotinin did not prevent macroaggregate formation.
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1985.tb01120.x
出版商:Blackwell Publishing Ltd
年代:1985
数据来源: WILEY
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3. |
In vitro and in vivo Activities of Different Immunoglobulin G Preparations from the Rabbit against Enterobacteriaceae |
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Vox Sanguinis,
Volume 49,
Issue 4,
1985,
Page 267-276
M. Trautmann,
Y. Müller‐Leutloff,
T. Hofstaetter,
F.R. Seiler,
H. Hahn,
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摘要:
AbstractSpecific rabbit IgG, prepared against three enterobacterial strains, was modified according to procedures used for the production of human IgG preparations suitable for intravenous infusion. The resulting products were examined for their in vitro opsonic and in vivo protective activity against the respective bacterial strains. A sulfonated IgG preparation (S‐sIgG) and the enzymatically derived fragments F(ab')2and Fab/Fc were opsonic in vitro and provided in vivo protection against lethal enterobacterial infection in mice. Protection by Fab fragments varied in the different in vivo model
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1985.tb01121.x
出版商:Blackwell Publishing Ltd
年代:1985
数据来源: WILEY
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4. |
A New Red Blood Cell Cold Autoantibody (Anti‐Me) |
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Vox Sanguinis,
Volume 49,
Issue 4,
1985,
Page 277-284
A. Salama,
H. Pralle,
C. Mueller‐Eckhardt,
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摘要:
AbstractA new red blood cell (RBC) cold autoantibody (anti‐Me) is presented which was detected in a patient with autoimmune hemolytic anemia of ‘cold type’ associated with Waldenström's macroglobulinemia. The autoantibody was of the IgM (Kappa) class, had a moderate titer, and reacted with cord as well as with adult RBC. Its hemagglutinating activity against RBC was markedly increased in the presence of preheated human milk, but not in the presence of simple sugars or preheated casein. The data suggest that the antibody is directed against a new (glycolipid) antigen common to human RBC termed Me antigen (‘milk en
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1985.tb01122.x
出版商:Blackwell Publishing Ltd
年代:1985
数据来源: WILEY
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5. |
Deficiency of Autologous Mixed Lymphocyte Reaction in Patients with Idiopathic Autoimmune Hemolytic Anemia |
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Vox Sanguinis,
Volume 49,
Issue 4,
1985,
Page 285-291
Roberto Conte,
Pier Luigi Tazzari,
Carlo Finelli,
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摘要:
AbstractAlterations in the autologous mixed lymphocyte reaction (autologous MLR) have been reported in many autoimmune diseases and in diseases with a derangement of T regulatory function. We have studied autologous MLR in 10 patients with idiopathic autoimmune hemolytic anemia (IAHA). All patients had decreased autologous MLR which averaged 4,106+1,332 cpm (SEM) compared to 12,153+4,166 cpm for simultaneously studied controls. A marked decrease in phytohemagglutinin response and an imbalance of T‐cell subsets were also observed. These findings suggest a possible abnormality of T immuno‐regulatory function in IAHA patie
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1985.tb01123.x
出版商:Blackwell Publishing Ltd
年代:1985
数据来源: WILEY
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6. |
Antiglycolipid Antibodies in Normal and Pathologic Human Sera and Synovial Fluids |
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Vox Sanguinis,
Volume 49,
Issue 4,
1985,
Page 292-300
Shunji Kaise,
Tatsuji Yasuda,
Reiji Kasukawa,
Tomoe Nishimaki,
Shinobu Watarai,
Toru Tsumita,
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摘要:
AbstractAntiglycolipid antibodies were measured in normal and pathologic sera and synovial fluids by means of a modified microplate method of complement‐mediated immune lysis of fluorescent dye‐trapped liposomes. All sera of normal subjects had antibodies against globopentaosylceramide (IV3GalNAcGbOse4Cer), ganglioside GMI, gangliotriaosylceramide, gangliotetraosylceramide, and galactosylneolactotetraosylceramide antigens. Most sera of normal subjects had antibodies against lactotriaosylceramide, N‐glycolylneuraminosyl‐neolactotetraosylceramide (NeuGcnLcOse4Cer), GM3ganglioside with N‐glycolylneuraminic acid (NeuGcGM3) and GDIaantigens. Differences of titers against IV3GalNAcGbOse4Cer, neolactotetraosylceramide, NeuGcGM3and NeuGcnLcOse4Cer antigens were observed between sera of normal subjects and pathologic sera from cases of leukemias, lymphomas, several autoimmune diseases and liver
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1985.tb01124.x
出版商:Blackwell Publishing Ltd
年代:1985
数据来源: WILEY
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7. |
Autoimmune Hemolytic Anemia Due to Anti B Autoantibody |
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Vox Sanguinis,
Volume 49,
Issue 4,
1985,
Page 301-303
Vichai Atichartakarn,
Pimol Chiewsilp,
Pimolparn Ratanasirivanich,
Sunee Stabunswadgan,
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摘要:
AbstractAn anti B autoantibody was found during a routine cross‐matching of the blood of two patients who presented with fever and hemolytic anemia. It appeared transiently and was shown to be IgM‐ and cold‐reacting. The patients had an excellent response to a short course of corticosteroid trea
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1985.tb01125.x
出版商:Blackwell Publishing Ltd
年代:1985
数据来源: WILEY
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8. |
The Radioactive Sodium Chromate Method for Erythrocyte Survival |
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Vox Sanguinis,
Volume 49,
Issue 4,
1985,
Page 304-307
F.G. Ebaugh,
J. F. Ross,
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ISSN:0042-9007
DOI:10.1111/j.1423-0410.1985.tb01126.x
出版商:Blackwell Publishing Ltd
年代:1985
数据来源: WILEY
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9. |
Erratum |
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Vox Sanguinis,
Volume 49,
Issue 4,
1985,
Page 308-308
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ISSN:0042-9007
DOI:10.1111/j.1423-0410.1985.tb01128.x
出版商:Blackwell Publishing Ltd
年代:1985
数据来源: WILEY
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