摘要:

Abstract.A quadruple plastic bag system made of Teruflex® PVC (Terumo) was tested. Red cells separated from plasma and buffy coat were resuspended in SAGM solution. After storage for 42 days, the 24‐hour posttransfusion autologous survival was 73.3±6.6% (X̄ ± SD), range 61.8–80.7%, n = 10. The shape of the cells was changed so that normal or slightly abnormal morphology was found in 37±7% of the cells after 42 days. Spontaneous in vitro hemolysis was 0.63±0.32% (range 0.28–1.13). A sufficient glucose reserve still remained at the end of storage, but glucose consumption and lactate production were impaired during the last 2 weeks due to the increasing acidity. By extrapolation from other studies it is suggested that 35 days is a more suitable shelf life than 42 days in

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1985.tb00790.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

Abstract.There is continuous need for blood components with long shelf life. To this end the ‘circle’ triple pack has been modified to provide 35‐day red cell and 7‐day platelet concentrates, in PVC bags of identical formulation and 0.4 mm thick. The plasticizer is a mixture of DEHP and TOTM (CLX Mark II). The primary pack contains 63 ml of CP 277.5 mMglucose. The third pack contains 100 ml of citric acid 2 mM, trisodium citrate 20 mM, NaH2PO420 mM, NaCl 123 mM, glucose 40 mM, and adenine 1.26 mM. Plasma is adenine‐free. With these modifications both hard‐ and soft‐spun red cells gave satisfactory biochemical and autologous survival indices up to 35 days of storage, the haematocrit not exceeding 60%. Platelet concentrates were acceptable at 7 days

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1985.tb00791.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

Abstract.Coagulation factor VIII and IX concentrates produced in Scotland inhibited human lymphocyte transformation induced by lectins or the recall antigen, purified protein derivative of tuberculin (PPD). Although concanavalin A could bind factor VIII directly and PHA could bind factor IX directly, most of the inhibition was not due to direct clotting factor – lectin interaction nor to simple toxicity. Most of the inhibitory activity from both clotting factors could be removed by dialysis. A similar degree of inhibition was observed when the buffers used to prepare the concentrates were substituted for the concentrates themselves, and a comparable concentration of sodium citrate was also found to be inhibitory. Coagulation factor VIII partially purified by gel filtration was not found to have an appreciable effect on lymphocyte transformation in vitr

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1985.tb00792.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

Abstract.The method described here using a centrifuge and Coulter Cell Counter for the quantitative differential agglutination of human red cells uses commercial anti‐A and anti‐B antisera for the ABO system, and for the Rh system a commercial anti‐D serum, a low ionic strength solution and an anti‐human IgG antiserum. We compared this Coulter Counter method with the Technicon AutoAnalyzer method which utilizes bromelin and polyvinyl pyrrolidone, and anti‐A, anti‐B and anti‐CD antisera, and found this new method to be the simpler of the two. The nonagglutinable count with the Coulter Counter was 1.07% for A1red cells, 2.26% for A2red cells, 1.06% for B red cells, and 1.78% for Rh‐positive red cells, results similar to those seen with the Technicon AutoAnalyzer.Results with the Coulter Counter method were consistently accurate whether the ACD red cells were studied on the day of collection, after 10 days of 4 °C storage, or after 4 °C storage for up to 6 days followed by cryopreservation with 40% (w/v) glycerol at–80 °C, thawing and washing.In this study, red cell samples obtained from recipients who had received compatible but identifiable donor red cells were frozen with 40% glycerol and stored at–80 °C for 10 months, thawed and washed. Survival measurements on these washed previously frozen red cells were similar to the values in

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1985.tb00793.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

Abstract.An assay has been developed to distinguish active from passive Rho(D) immunization in a patient who had recently received hyperimmune anti‐Rho(D) immunoglobulin therapy. Isolated peripheral B lymphocytes from a pregnant woman at 32 weeks gestation were co‐cultured with Epstein‐Barr virus in a liquid growth medium. After 7 days, anti‐Rho(D) antibodies produced in vitro by the transformed lymphocytes were detected in culture supernatants, thereby proving active immunization and indicating the potential of hemolytic disease of the newborn in the current pregnancy. This assay was also performed with peripheral B lymphocytes from three groups of individuals: mothers known to be Rho(D) immunized and who recently delivered Rh‐positive infants, women with longstanding Rho(D) immunization, and women who were treated with anti‐Rho(D) globulin. In the first group, anti‐Rho(D) antibodies were again detected after in vitro viral stimulation. In the latter two groups, essentially no anti‐Rho(D) activi

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1985.tb00794.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

Abstract.An allo‐anti‐P1antibody is reported in an elderly Caucasian male with P1+ red cells. There was no suggestion that the antibody was causing haemolysis. The case suggests that a slight structural variation may exist in the patient's B antigen giving a serological false‐positive response when typed with reagent anti‐P1, or that the patient's anti‐P1is directed against a determinant absent in the patient's own antigen

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1985.tb00795.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

Abstract.During routine pretransfusion testing, the presence of IgG on patient red cells is suggested by a positive autocontrol and confirmed by a positive direct antiglobulin test (DAT) using monospecific anti‐IgG sera. Most IgG on patient red cells detected in this manner are of unknown etiology. We recently showed an association between elevated serum globulin levels and positive DAT with unreactive eluate in patients with acquired immunodeficiency syndrome (AIDS). In the present study, we wished to determine whether elevated serum globulin levels contribute to some of the positive DAT encountered in pretransfusion testing of patients without AIDS. 76 patients with positive DAT were compared with 90 controls without IgG detected on their red cells during pretransfusion testing. The rate of elevated serum globulin levels was 75% in positive DAT cases versus 29% in controls (p<0.001); the odds ratio was 7.6. Elevated blood urea nitrogen levels occurred in 42% of cases versus 19% of controls (p<0.025); the odds ratio was 3.1. Cases and controls were not significantly different with regard to age, sex, race, quinidine usage, or hyperalimentation. Elevated serum globulin and blood urea nitrogen levels are significantly associated with a positive DAT with unreactive eluate in pretransfusion patient

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1985.tb00796.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

Abstract.900 pregnancy sera were screened for monocyte antibodies. 23 sera (2.6%) were found to be reactive with monocytes in an allotypic pattern distinct from blood group ABO, HLA‐A,B,C, DR and DQ specificities. Because of strong reactions and high reproducibility, 4 sera with pure endothelial monocyte (EM) reactivity and 2 sera also positive with B lymphocytes of certain donors were selected for population and family studies. The frequency of positive reactions in a panel of 26 random donors obtaied with the pure EM sera was 13,8,8 and 13% with clearly distinct patterns. Analysis of the tentatively designated EM antigens 1.1, 1.2, 2.1, and 2.2 in 7 families revealed definite segregation with HLA. By means of two crossing‐over events within the HLA region the two hypothetical EM loci were localized centromeric of HLA‐A and one of both loci centromeric of

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1985.tb00797.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

Abstract.Antibodies to HTLV‐III have been investigated in 118 multitransfused β‐thalassemia patients. Thirteen patients (11.01%) were found to be positive; 3 of these 13 showed clinical and immunological signs of the lymphadenopathy syndrome. A retrospective study carried out on 65 sera has shown that at least 6 patients were negative 3 years before the present investigation. This is the first extensive study on HTLV‐III infection in multitransfused β‐thalassemics. It suggests that these patients are at risk for the acquired immune deficiency syndrome and related

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1985.tb00798.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1985.tb00799.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY