摘要:

Abstract.Immune serum globulin (ISG) prepared by Cohn cold alcohol fractionation of pooled human plasma was reduced with dithiothreitol (DTT) and alkylated with iodoace‐tamide and other alkylating agents. Our results show that there are a few labile inter heavy chain disulfide bonds in ISG which react rapidly under mild, non dissociating conditions. The extent of disulfide cleavage is controlled primarily by the ratio of DTT to ISG until about 4–5 disulfide bonds have been reduced. We report detailed studies on the variables of ISG concentration, DTT to ISG ratio, pH, and time, leading to a chemically modified ISG that has a controlled and limited number of reduced and alkylated disulfide bo

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1981.tb00725.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Abstract.The commercial immune serum globulin (ISG) product is suitable for use in passive immunization only by intramuscular administration; intravenous use results in vasomotor side reactions. We report the functional characterization of a chemically modified ISG (MISG) that is essentially free of nonspecific complement‐binding activity and contains no new antigenic determinants. The half‐life values for MISG given intravenously to rabbits, guinea pigs, and humans are shown not to be different from that of ISG. The MISG we describe is shown to fulfill the in vitro and in vivo criteria of stability, safety, function, and half‐life, making it suitable for human intravenous administr

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1981.tb00726.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Abstract.We examined the binding capacity of radioiodinated protein A ([125I] PA) for several human IgG immunoglobulins. Most of them are commercial preparations used in immunosubstitution therapy. Two experimental approaches were chosen. In the first, the binding of [125I] PA to solidified IgG preparations was studied. In the second approach, the inhibition of [125I] PA binding to a given immunoglobulin by various human immunoglobulin samples was established. Both the binding and inhibition criteria were used to estimate the order of the relative binding capacities of the Fc region in studied IgG preparations. The various commercial gamma‐globulin preparations were also compared using single radial immunodiffusion and cross‐immunoelectrophoretic techniq

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1981.tb00727.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Abstract.McLeod phenotype red cells of the Kell blood group system have acanthocytic morphology and reduced in vivo survival. The phenotype has an X‐linked mode of inheritance and is found in some males who have no abnormality of leukocyte function and in some who have X‐linked chronic granulomatous disease (CGD). We now describe an association between the McLeod phenotype and an abnormal elevation of serum creatine phosphokinase (CPK). The increase is of the MM isoenzyme type, derived from skeletal muscle or cardiac muscle, and muscle biopsy shows evidence of muscle cell changes. All of 11 males who have McLeod syndrome but do not have CGD have high levels of serum CPK. Males with McLeod syndrome and CGD may have normal or high levels of the enzyme. Individuals with other variant phenotypes in the Kell system have normal levels of serum CPK. Studies on a large kindred, which includes 5 people of McLeod phenotype, show high CPK levels only in the members of McLeod type. We conclude that the high level of CPK in the serum of these people is a reflection of a muscle cell anomaly and that in these individuals it is a pleiotropic effect of the X‐linked gene that produces the McLeod red cell phen

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1981.tb00728.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Abstract.Anti‐Meis an antibody which cross‐reacts with the M and He antigens. It has previously only been found in rabbit serum; the first human example is described.The Henshaw antigen is closely associated with the MNSs system and is found in about 3% of Negroes [1–3] but only rarely in Caucasoids [2]. The original anti‐He was found in a rabbit anti‐M serum byIkin and Mourant[1] in 1951. Other examples were deliberately produced by the immunisation of rabbits with the blood of Mr. Henshaw [3].The first example of anti‐He in a human serum was found in 1967 byMacDonaldet al. [4] in a Caucasoid mother. Although at the time it was not possible to ascertain the racial group of the father, it has since been established that he was a Negro. Other human anti‐He sera have since been found [2].In 1961Wiener and Rosenfield[5] reported an immune rabbit serum which possessed inseparable anti‐M and anti‐He specificities which they designated anti‐Me. The rabbit which produced the anti‐Mehad almost certainly not received Henshaw‐positive cells. The rabbit serum was investigated as a result of a discrepancy found during M‐typing of a Negro involved in a medicolegal case of disputed parentage.The first human example of anti‐Meherein described was found in the serum of Mr. Richards, a 41‐year‐old Caucasoid male with no known transfusion history and has been used at this centre as

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1981.tb00729.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Abstract.A 23‐year‐old male patient undergoing first induction chemotherapy for acute myeloid leukaemia received granulocyte transfusions on 10 consecutive days. An average of 2.1times1010granulocytes was given per square metre body surface area per day. Transfusion reactions and absence of post‐transfusion granulocyte increment after the fifth granulocyte transfusion suggested the presence of leukocyte antibodies. However, no antibodies were detectable at this time by the lymphocytotoxicity test, the indirect granulocyte immunofluorescence test, or the platelet suspension immunofluorescence test. In contrast to the clinical observation, the serological detection of leukocyte antibodies was only possible 1 day after the last granulocyte transf

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1981.tb00730.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Abstract.Genetic polymorphism of human plasminogen in the Japanese population was studied using agarose gel isoelectric focusing followed by immunofixation. A new basic variant, PLG B, was found as a heterozygous state PLG l‐B, which was genetically determined. The allele frequencies calculated from 258 individuals werePLG1=0.958,PLG2=0.020 andPLGB=0.02

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1981.tb00731.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY