摘要:

Abstract.Platelet‐associated IgG (PAIgG) and IgM (PAIgM) levels were quantitated in patients with thrombocytopenias of various etiologies. 32 of 34 patients with immune thrombocytopenia had elevated PAIgG, 8 of the 34 patients had elevated PAIgM levels. Only 1 patient suffering from immune thrombocytopenia had elevated PAIgM with normal PAIgG levels. Elevated PAIgM was most often associated with elevated PAIgG except for the following: 2 patients with lymphoproliferative disorders, 1 patient with heparin‐induced thrombocytopenia, 1 patient with pernicious anemia, and 2 patients with Waldenström's macroglobulinemia. PAIgM determinations may prove to be a useful adjunct for evaluating patients with thrombocytopenia, particularly those in whom the PAIgG is not elev

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1983.tb01893.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Abstract.Hybridomas secreting monoclonal antibodies to hepatitis B surface antigen (HBsAg) were established by fusion of the spleen cells from mice immunized with purified HBsAg with the mouse myeloma cell line P3‐NSI/l‐Ag4‐l. The monoclonal antibodies to the group‐specific antigen (a) produced by one of them were used for the automated screening of HBsAg on the Groupamatic 360 (Kontron International). Its sensitivity is almost equal, but slightly inferior, to the system employing polyclonal horse antibodies to HBsAg; it barely detects 6 ng/ml of HBsAg. It is also as highly specific as the system with polyclonal antibodies; the incidence of false‐positive reactions is 0.2%. These results indicate that the monoclonal antibodies will become a practical source for the HBsAg screening on the Gr

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1983.tb01894.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Abstract.Screening tests for hepatitis B virus (HBV) markers were performed in 266 hematologic patients in order to evaluate the role of transfusion therapy in HBV infection and to identify other possible causes of the high rate of HBV markers positivity in onco‐hematologic units. As control groups we tested 99 nonhematologic polytransfused patients, 66 nonhematologic, nontransfused inpatients with various diseases and 72 subjects randomly selected from the general population. Higher HBV markers prevalence was found in hematologic patients, nonhematologic polytransfused patients and nonhematologic, non‐transfused inpatients than in the general population. HBV markers prevalence correlated with the length of hospitalization in all inpatients studied. Our data suggest that hospital admission is a major factor in HBV transmission in hematologic patients and in other inpatients studied. Blood transfusions represent a risk factor only when utilized as chronic treatm

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1983.tb01895.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Abstract.30 asymptomatic chronic carriers of hepatitis B surface antigen (HBsAg), 6 asymptomatic blood donors transiently infected with hepatitis B virus, and 38 patients with acute hepatitis B were tested for HBsAg, anti‐HBs, HBeAg, anti‐HBeand anti‐HBc.Comparison of these results revealed significant variation in the frequency of HBeAg which was present in 1 (3.3%) carrier, 2 (33.3%) of the transiently positive donors, and in 24 (63.2%) of the patients with acute hepatitis. Anti‐HBewas found in 28 (93.3%) of the carriers, 4 (66.6%) of the transiently positive donors, and in 8 (21%) of the patients. Variation was also seen in the strength of anti‐HBc, with only the chronic carriers having titres which were consistently high (above 1,000). Retesting the two groups of donors after a period of approximately 2 years showed no change in the serologic status of the chronic carriers, while amongst the transient HBsAg positives the 2 HBeAg reactives had seroconverted, 1 of the anti‐HBepositives had become non‐reactive, and 2 of the 6 had developed anti‐HBs. 6 of the patients with acute hepatitis B were serologically reexamined during convalescence and showed results similar to those seen in the transiently HBsAg‐positive donors, with clearance of HBsAg in all, seroconversion from HBeAg to anti‐HBein 4, and the product

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1983.tb01896.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Abstract.Detailed serological investigation of five examples of anti‐Jkarevealed considerable heterogeneity in their characteristics. All the sera were investigated by the antiglobulin technique, with and without complement and by a range of sensitization and testing methods. Three were readily detected in the presence of complement, while two were detectable with anti‐IgG, providing a spin test was used. Complement was bound by all and resulted in enhanced reactions, particularly when slide/tile tests were used. The study emphasizes that anti‐complement (C3) is an essential component of polyspecific antiglobulin reagents used in cross‐matching, enabling the ready detection of these clinically important ant

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1983.tb01897.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Abstract.The clinical histories of 30 consecutive Rh0D‐negative oncology patients transfused with Rh0D‐incompatible platelet concentrates were analyzed. No evidence of sensitization to the Rh0D antigen was found whatsoever, in spite of the large numbers of Rh0D‐incompatible platelet transfusions given to these patients. The results of this work seem to indicate that patients with malignant diseases, who are undergoing various forms of antineoplastic therapy, are immunomyelosuppressed, and have severe thrombocytopenia, can safely receive platelets from Rh0D‐positive donors without developing evidence of immunization to Rh0D a

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1983.tb01898.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Abstract.S‐sulfonation represents a reversible chemical modification of disulfide bonds by which under the special conditions chosen only about 2.2 cystine units per IgG molecule are cleaved. Physicochemical and functional evidence for reconstititution is presented. Molecules reconstituted in vitro or in vivo regain, within a few hours, a reactivity (antigen binding, immunoprecipitation, Clq‐mediated cross‐linking of immune complexes) comparable to equimolar control prepara

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1983.tb01899.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Abstract.The generation and release of mediators of inflammation and anaphylaxis via activation of complement or of Fc receptor‐bearing cells is held responsible for adverse reactions observed upon intravenous administration of standard immunoglobulins. They are caused by immunoglobulin G (IgG) effector functions predominantly located in the Fc region of the molecule and ‘activated’ by aggregate formation. Their functional activity depends on the correct conformation of the Cγ2 domains of Fc and are therefore impaired or even abrogated by S‐sulfonation of the hinge disulfide bonds, as demonstrated in this communication: S‐sulfonated IgG (S‐IgG) has no anticomplementary activity and does not interact with Fc‐receptors anymore. After antigen binding, i.e. immune complex (IC) formation, sulfonated IgG is about half as potent as standard IgG in complement activation or phagocyte stimulation (human monocytes and granulocytes). The two activities synergize, however, so that in the presence of complement S‐IgG IC are as effective phagocyte activators as standard IC. Moreover, S‐sulfonation being the only chemical modification of imunoglobulins that is reversible, it can be demonstrated that all IgG effector functions important for antigen removal are regained by reconstitution of t

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1983.tb01900.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1983.tb01901.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1983.tb01902.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY