ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1981.tb00732.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Abstract.Polystyrene beads coated with pasteurised polyethylene glycol‐precipitated HBsAg were used to detect anti‐HBs by a solid phase inhibition assay employing unreacted125I‐anti‐HBs which had previously been ‘processed’ by polyethylene glycol precipitation and used to test for HBsAg. A weak anti‐HBs (approximately 0.1 IU/ml) positive control was used to establish a target level of reactivity. 16,035 freshly donated units of blood, a 90‐sample coded anti‐HBs panel and a selection of hyperimmune globulins were investigated. All positive results were confirmed by a standard commercial assay. Antibody content of selected samples was quantitated in international units. A target level of anti‐HBs activity is suggested for donor plasma to be fractionated for the production of hepatiti

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1981.tb00662.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1981.tb00733.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Abstract.Approximately 90% of 67 multi‐transfused patients with bleeding disorders were positive either for anti‐HBs (85%) or HBsAg (4.5%). Using a polyethylene glycol trypsinization assay, we found HBsAg‐containing specific circulating immune complexes (HBsAg‐CICs) in 3 of the 57 HBsAg‐negative haemophiliacs possessing anti‐HBs. The occurrence of HBsAg‐CICs may be a regular event in the conversion phase of HBs infection. Circulating immune complexes as detected by the anticomplementary assay were found in 32 of the 67 (

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1981.tb00663.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Abstract.Red cells of the McLeod phenotype in the Kell blood group system have an acanthocytic morphology. The membrane protein composition analyzed on sodium dodecylwlfate‐polyacrylamide gel electrophoresis, the ATP level and the activities of a large number of intracellular enzymes appear to be normal. Membranes prepared from McLeod red cells incubated with γAT[32P] and MgCl2incorporated twice as much radioactivity into spectrin and also showed a slight elevation of phosphorylation in band 3 protein when compared to membranes from normal cells. Intact normal red cells incubated with carrier‐free [32P] incorporated radioactivity into several proteins. with most incorporation in spectrin and band 3 protein. In comparison, McLeod cells incorporated three times more radioactivity into spectrin and band 3 protein but increased phosphorylation also occurred in other, but not all, membrane proteins. Intact McLeod red cells also showed increased phosphorylation of membrane phospholipids, but they incorporated [32P] into intracellular nucleotide phosphates in a normal ma

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1981.tb00664.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1981.tb00734.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1981.tb00735.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Abstract.In an attempt to provide an explanation for the previously reported effect of pregnancy on the Lewis phenotype of erythrocytes, the level of Leb‐active glycolipid in serum was compared with the reactions of erythrocytes, using samples obtained from 73 nonpregnant women, 74 women at the time of delivery, and 2 women at weekly intervals during their pregnancy. In this Swedish population, the frequency of the Le(a‐b‐) blood group increased from 11% in nonpregnant women to 36% in women at the time of delivery. Among Le(a‐b+) women of all ABO groups, those who were A1most often became Le(b‐) during pregnancy. The change in phenotype occurred as early as the 24th week of gestation; the Lebantigen was again detectable within 6 weeks after delivery. The concentration of Lebglycolipid in serum, as measured by radioimmunoassay, decreased only slightly during pregnancy. The repartition of glycolipids, secondary to the increased ratio of lipoprotein to red cell mass that occurs during pregnancy, may account for the relative lack of Lewis glycolipid on ery

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1981.tb00665.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Abstract.The trypsin‐polybren‐citrate (TPC) technique is based on Lalezari's method and has been developed on the Groupamatic equipment to allow the screening of irregular alloantibodies which are not detectable on this machine by the present routine techniques. TPC screening has two main advantages: it gives more reliable results for Rh, Kell, Lewis and P antibodies than bromelin‐methyl‐cellulose, and it permits the screening of Duffy and Kidd antibodies. However, although the TPC technique contributes to an improved quality of the automated screening of blood donor samples, it should not be used as the only method when recipient samples are co

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1981.tb00666.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1981.tb00736.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY