摘要:

Abstract.We have shown that the first component of complement C1 is present in an active form on the surface of washed human peripheral lymphocytes but not on platelets or erythrocytes. This active C1 (C1‐) was detected by its ability to transfer to sensitized cells carrying C4, i.e., EAC4, forming EAC1‐,4. Active C1 was also able to consume C4. Treatment of these lymphocytes with 0.02 m EDTA removed C1. EDTA‐treated lymphocytes were able to bind exogenous purified human C1. Comparative studies with sentized erythrocytes (EA) and EDTA treated lypmhocytes showed that although fewer molecules of exogenous C1 could bind to the EDTA‐treated lymphocytes than to EA, the consumption of C4 by C1‐bound to lymphocytes was significantly higher than that observed with EAC1‐. When lymphocytes obtained from 2 patients with chronic lymphocytic leukemia and hypocomplementemia were tested, the release of C1, the C4 consumption and the binding of C1‐to EDTA‐treated cells were hig

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1978.tb02922.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

Abstract.When granulocytes are collected by either discontinuous‐flow centrifugation or filtration leukapheresis, lysozyme is released. More lysozyme is released during the filtration procedure than during the centrifugation procedure. A small amount of red cell lysis occurs during the centrifugation but not the filtration procedure. A rise in lactate dehydrogenase levels consonant with the amount of hemolysis is observed. These findings suggest that granulocytes collected by either filtration or discontinuous‐flow centrifugation undergo degranulation but not lysis sufficient to cause cytoplasmic enzyme release during the donat

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1978.tb02923.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

Abstract.The first case of interdonor incompatibility associated with granulocyte transfusion is presented. The patient received Kell positive granulocyte transfusions containing about 30 ml of red cells 36 and 132 h prior to receiving a granulocyte transfusion containing anti‐Kell 1/128. The chills, fever, vomiting and hypotension resulting from the red cell incompatibility, cleared with appropriate fluid theiapy. Antibody detection methods must be applied to each unit of granulocytes. The patients blood should be tested with reagent grade antibody to detect small numbers of antigen‐containing cells if an antibody‐containing granulocyte transfusion is to be given. Routine major and minor cross‐matching is insufficient. Removal of the antibody containing plasma and resuspension of the granulocytes in plasma free of irregular antibodies may be the most effective way to prevent such incompat

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1978.tb02924.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

Abstract.A sandwich enzymoimmunoassay (EIA) procedure was developed for the detection of HBsAg using Fab' of anti‐HBsAg conjugated with β‐D‐galactosidase fromEscherichia coliwith anti‐HBsAg‐coated silicone rubber discs as a solid phase. EIA could detect 1 ng/ml of HBsAg. It was as sensitive as radioimmunoassay (RIA, AusRia II) and about 30‐fold more sensitive than reversed passive hemagglutination assay RPHA, ReverseCell). EIA and RIA could detect more HBsAg‐positive

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1978.tb02925.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

Abstract.Hepatitis B surface antigen (HBsAg), was purified from plasma by affinity chromatography on matrix‐bound sulphated carbohydrates such as heparin or dextran sulphate. Further purification by precipitation with polyethylene glycol (PEG) 6000 resulted in a highly purified HBgAg preparation. The overall recovery amounted to about 70% of the total antigen content of the starting plasma. Electron microscopic data revealed mainly 22 nm spherical particles accompanied by few or no filaments. The process is simple, rapid and lends itself readily to large‐scale applicati

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1978.tb02926.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

Abstract.Lymphoid tissues from 24 human fetuses were assayed for hemolytic plaque‐forming cells (PFC) against a variety of erythrocyte targets. PFC against maternal and other erythrocyte antigens were commonly detected in human fetal liver, lymph nodes, spleen, or thymus as early as 16 weeks gestation and were usually more abundant in liver than in spleen after 16 weeks gestation. These data corroborate studies from other laboratories which indicate that human fetuses develop some forms of immunocompetence very early during gestatio

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1978.tb02927.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

Abstract.Extracts of seeds from 79 plant species were tested for agglutinating and lytic activity with the red cells of ten vertebrate species (man, monkey, rabbit, rat, goat, sheep, guinea pig, horse, fowl, dog). Of these extracts, 24 were hemagglutinating and 15 were hemolytic. The results suggest that some lectins can serve as useful diagnostic reagents in distinguishing the blood of one animal species from that of another.

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1978.tb02928.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

Abstract.The AutoAnalyser Technicon II system was adapted for screening red cells for the presence of warm‐type autoantibodies. A significant decrease in optical density was recorded when the red cells of 38 patients with autoimmune haemolytic anaemia were tested using the bromelin‐methylcellulose method. This was never seen with the red cells of 226 patients with other diseases or of healthy blood donors. The method is very simple and more sensitive than the manual direct antiglobulin t

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1978.tb02929.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

Abstract.Investigation of the inheritance of a ‘new’ low‐incidence red cell antigen (Fra) indicates that it is an autosomal dominant marker and not part of the ABO, MNSs, P, Rh, Kell, Lutheran, Duffy, Kidd, Dombrock or Scianna blood group systems. No genetic linkage was established between 3Fr, the locus governing production of Fra, and any of 21 other ma

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1978.tb02930.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

Abstract.Mild as well as strong periodate oxidation of isolated erythrocyte N and M glycoproteins and glycopeptides gave extensive to complete destruction of N‐specificities as measured withVicia gramineaextracts and of N‐ as well as M‐ activities determined with all but 1 of 8 animal anti‐N and 13 anti‐M sera. Results with human antisera differed somewhat, while the specificity of mildly oxidized N‐glycoprotein was completely destroyed as determined with all 8 human anti‐N sera used, that of strongly oxidized N‐active substance was completely inactivated towards one of the human antisera; the remainder showed 63–94% destruction, and 2 sera indicated no effect of oxidation. Similarly, while 10 of 14 human anti‐M indicated complete inactivation of M‐specific glycoproteins and glycopeptides after mild or strong oxidation, 2 showed partial inactivation and 2 human anti‐M sera showed no inactivating effect of oxidation. The most relevant findings of quantitative carbohydrate analysis of periodate oxidized N‐ and M‐specific substance were extensive transformation of N‐acetylneuraminic acid (NAN) to its C8and C1analogues on mild oxidation and pronounced destruction of NAN and its analogues on strong oxidation; however, some intact NAN always remained. In all instances galactose (Gal) was destroyed to a much larger extent in N‐derived than in M‐derive

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1978.tb02931.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY