摘要:

A substantial number of individuals typed as HLA‐B17 do not have Bgb. To determine whether or not this discrepancy reflects genetic or ethnic group differences or the influence of B17 antigen subtypes, a large number of unrelated and related B17 individuals from the Caucasian, Hispanic, Black and Chinese ethnic groups were tested in parallel for Bgband Bw57‐ or Bw58‐ B17 subtype. Higher Bgbexpression was found in association with Bw57, but differences in expression of Bgbwere also seen in different ethnic groups and in different related individuals carrying the same Bw57 or Bw58 haplotype. Genetic factors other than HLA thus influence the expression of HLA antigens on red cells. Standard and antiglobulin lymphocytotoxicity tests indicate that all Bgbtyping sera contain anti‐Bw57 antilymphocyte antibodies and most also contain anti‐Bw58 and cross‐reacting anti‐B12, B15 and/or Bw

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1982.tb01109.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

This study was initiated in order to assess any immunologic effects that source leukocyte concentrate collection might have on double‐bag plasmapheresis donors. Previous studies have shown that surveillance parameters, such as T versus non‐T lymphocyte subpopulations, showed no abnormal values in donors with as many as 500 visits over a 12‐year period. The present study demonstrates that the frequency and the total number of leukocyte donations do not effect the lymphocyte subpopulations and functions observed. No significant changes were noted for specific and nonspecific stimulation, natural killer (NK) cell activity, lymphocyte surface markers and a variety of functional parameters. For example, the apheresis donors manifested no differences in NK cell activity, human leukocyte interferon production, IgG synthesis by B cells and percent suppression of both IgG synthesis and mixed lymphocyte cultures, when compared to non‐apheresed

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1982.tb01110.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

Platelet concentrates were obtained from blood anticoagulated with CPDA‐1 and their viability after storage in CL‐3000 containers for 72 h at 22°C and for 24 and 48 h at 4°C was studied using autologous reinfusion of51Cr‐labeled platelets. Yield and t1/2values after such storage were similar to those previously reported for other containers and anticoagulants. Survival of platelets stored at 22°C for 3 days was essentially normal with the expected yields for that duration of storage. As has been described for other containers, adenine has no adverse or beneficial effect on platelet viability when assessed with these

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1982.tb01111.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

Human red blood cells (H‐RBC), sensitized by specific rabbit antihuman hemolysin, showed a significant increase in susceptibility to complement lysis after rigidification of the membrane lipid layer by incorporation of cholesterol (p<0.001). Conversely, fluidization of the membrane lipid layer by treatment with phosphatidylcholine for cholesterol depletion displayed increased tolerance to the complement lysis (p<0.01). Antisera, obtained from 6 rabbits immunized with untreated H‐RBC, were tested separately. The average serum concentration, exerting 50% hemolysis, was 1.1 ± 0.4% for cholesterolenriched H‐RBC and 16.3 ± 7.5% for cholesterol‐deple

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1982.tb01112.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

The in vivo survival of Co(a+b‐) red cells in a patient with anti‐Coawas studied using a small aliquot of51Cr‐labeled red cells. The antibody reacted at room temperature without incubation and after completion of the antihuman globulin test with both reactions enhanced by ficin. Circulation of transfused cells was shortened with a T50of 5 min. The patient's anti‐Coatiter rose following transfusion to 1:256. These data suggest that individuals with anti‐Coashould receive Co(a‐b+) red cells for

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1982.tb01113.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

A three generation family with 16 members shows that the Hey red blood cell antigen is not in the Rh, P or Kidd systems. The mother of the proband was found to have an IgG component in her anti‐Hey, probably the result of alloimmunization by her two Heypositive children. The wife of the proband who had 1 Hey‐positive child, and 4 recipients of a single unit of Hey‐positive blood did not have detectable anti‐Hey. The presence of the IgG component of the mother's anti‐Hey raises the possibility that both hemolytic disease of the newborn (HDN) and hemolytic transfusion reactions (HTR) could occur due to

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1982.tb01114.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

A study was made for the therapeutic removal of red cell antibodies using antigen positive red cells. With preliminary experiments in vitro, the criteria for the optimal absorption of anti‐M with O MM red cells were estimated to be the following: an incubation time of 10min, an incubation temperature of 0–4°C (in an ice‐water bath), and a red cell‐to‐plasma ratio of 1:2.5.On the basis of these in vitro experiments, the following procedure was designed. One unit (about 90 ml) of O MM packed red cells was added to a bag containing 250 ml of patient's plasma, the mixture was incubated in an ice‐water bath for 10 min. Following centrifugation, autologous plasma from which the antibodies were removed was reinfused. After absorption the titer of anti‐M fell from 512 to 4. No variation in the level of other plasma components was detected and no hemolysis was seen. After 2.5 *** of patient's plasma was treated using the above described method, the antibody titer usually decreased one log2unit.When this method was compared with exchange plasmapheresis, no significant abnormalities in the immunoglobulin and hemostatic factors could be detected, but a moderate decrease in platelet count was seen after plasmapheresis. Thus, it appears that our method provides a rational therapeutic modality for specifically remov

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1982.tb01115.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

We performed a follow‐up study on 70 patients with acute non‐A, non‐B (NANB) posttransfusion hepatitis and a retrospective study on 283 chronic hepatitis, 70 cirrhosis and 53 hepatocellular carcinoma patients of type NANB. In acute NANB posttransfusion hepatitis, as judged by the transaminase levels, the duration of the disease exceeded 6 months in 46/70 = 65.7% and 1 year in 32/70 = 45.7%. The histological diagnosis of the 32 cases persisting for more than 1 year was chronic active hepatitis in 5, chronic persistent hepatitis in 2 and unresolved hepatitis in 6. The frequency of previous transfusion in chronic hepatitis, cirrhosis and hepatocellular carcinoma of type NANB was 42.8, 37.1 and 15.1%, respectively, whereas the incidence of early posttransfusion hepatitis was 8.5, 8.6 and 7.5%, respectively. In chronic liver diseases with a history of jaundice and/or hepatitis, previous transfusions are more frequently associated with type NANB than with type B disease. The present study demonstrates that NANB posttransfusion hepatitis tends to develop to chronic liver disease when analyzed prospectively as well as retrospect

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1982.tb01116.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

Determination of the genetically controlled variants of the C3 system has been accomplished by electrophoresis on cellulose acetate membrane followed by immunofixation with a specific anti‐C3 antiserum. The results have been confirmed with the use of reference sera and compared with those obtained from conventional high voltage agarose gel electrophoresis. The interference of some electrophoretic variables on the technique has been investigated. The method is rapid, accurate, reliable and gives good separation of at least the three common C3 phenotype

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1982.tb01117.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1982.tb01118.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY