摘要:

Abstract.The distribution of four Gm antigens was determined in rivanol‐soluble as well as in chromatographically obtained human IgG preparations. In rivanol‐soluble IgG, enrichment in IgG3 carrying Gm5 antigen was demonstrated and, in a preparation fractionated by DEAE‐Sephadex chromatography, molecules with a predominantly Gml‐expressed antigen were shown to be

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1979.tb02301.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Abstract.Immunization of a chimpanzee, a gibbon, a baboon and a rhesus monkey with human globulins gave different results. While chimpanzees did not produce any anti‐human antibodies, baboon and rhesus monkey responded by producing anti‐human IgG antibodies that did not show subclass or allotype specificity. In the gibbon antiserum, on the other hand, six different antibodies were demonstrated with a specificity against one or more of the four human IgG subclasses, including one allotype.Comparative investigations of non‐human primate species sera showed that one of the specificities detected by these antibodies, i.e., the allotype Glm(f), was restricted to man; the other five antibodies reacted with man and with two or more genera of apes. Lower primates did not share any of these antigenic determinants. The extent of immunological distance is parallel to the taxonomic classification. The gibbon shows that there are more similarities between man and chimpanzee or pygmy chimpanzee than between man and gorilla or oran

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1979.tb02302.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Abstract.A low‐molecular‐weight preparation of hydroxyethyl starch (LMW‐HES) may serve as a desirable substitute for the erythrocyte sedimenting agents presently employed to improve neutrophil yields in centrifugation and gravity leukapheresis. The cell‐separating and erythrocyte‐sedimenting properties of LMW‐HES, assessedin vitroby the recovery of various blood cells in plasma supernatant fluids, were similar to those of the higher‐molecular‐weight hydroxyethyl starch in current use and to dextran under a variety of conditions. These data predict success for LMW‐HES as a sedimenting agent in leukapheresis and recommend that it be evaluated i

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1979.tb02303.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Abstract.Red cells and serum from two Bhvariants (B+H‐cells) have been investigated for B and H blood group glycosyltransferases. The H enzyme could not be detected using either type 1 or type 2 chain acceptors. The B enzyme was present in normal amount when 2′‐fucosyl‐lactose was used as substrate, neither 6′‐fucosyllactose nor 6′‐fucosyllactosamine could act as acceptors for the B enzyme.Upon treatment of the Bhred cells by the B‐degrading enzyme fromTrichomonas foetusthe B antigen was destroyed while H determinants were uncovered (B‐H+cells). The cells thus treated could be further converted into A+H‐ red cells by the action of the A transferase from human blood group A serum. Previous treatment of the B‐H+ cells by the H‐degrading enzyme fromT. foetus, however, led to B‐H‐ erythrocytes and prevented their conversion into A red blood cells by the A enzyme. The results clearly demonstrate that, as found in normal B individuals, the B antigen from Bhcells is built up from the H precursor and provide additional evidence thatHis not a completely

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1979.tb02304.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Abstract.The IgG and IgM anti‐A and anti‐B activities from several immune and non‐immune O, A and B sera were tested against a panel of weak A (A3, Ax, and Aend) and weak B (B3and Bx) red cells. In all cases it is the IgM which agglutinated optimally Ax(or Bx) cells, while IgG and IgM anti‐A (or anti‐B) reacted similarly with A3and Aend (or B3) cells. The agglutinating activity of all these ABO antibodies was found straightly related to their association constant for the A (or the B)

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1979.tb02305.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Abstract.Lods of +3.89 at a recombination fraction (Θ) of 0.10 and recombinant: non‐recombinant counts of2:16 indicate linkage between theRdandRhblood group loci. The possibility thatRdis the same as Sc is discuss

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1979.tb02306.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Abstract.Intensive plasma exchanges were carried out in a 34‐year‐old pregnant woman to lower the level of circulating anti‐D. The patient proved interesting from a number of serological aspects. The possible protective effect of coexistent ABO incompatibility is discussed. The effect of plasma exchanges on cytotoxic HLA antibodies is also demonst

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1979.tb02307.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Abstract.This report describes a Japanese family having two Rhmod members, and two sibs with slightly depressed Rh antigens. It is proposed that the Rhmodand the depressed Rh antigen phenotypes are due to regulator genes in homozygous or heterozygous condition, respectively.The two Rhmodmembers showed mild reticulocytosis, stomatocytosis, slight anisocytosis, slight hyperchromia and slight punctate basophilia without anaemia. Scanning electron microscopy showed their red cells to be small and of bowl shape. Increased fragility of their erythrocytes was demonstrated by dynamic osmofragility testing using a coil planet centrifuge.

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1979.tb02308.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Abstract.The frequency of Epstein‐Barr virus (EBV) and hepatitis B virus (HBV) infection has been studied in 149 polytransfused thalassaemic patients and in healthy controls. Evidence for EBV infection was based on the detection of antibodies to viral capsid antigen (anti‐VCA) and for HBV infection on the detection of either hepatitis B surface antigen (HBsAg) or hepatitis B surface antibody (anti‐HBs). The frequency of anti‐VCA was not significantly higher in the patients (16.4%) compared to the controls (69.8%) whereas HBV infection was more frequently observed in the patients (91.3%) than in the controls (17.3%). There was also no evidence of repeated infection or recent infection with EBV in the polytransfused patients. These data suggest that transfusion of stored blood does not represent a significant factor of spread

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1979.tb02309.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Abstract.Mostin vitrostudies on microaggregate filters focus on the mechanical retention of microparticles. Data are based on bulk techniques such as differential dry weight and screen filtration pressure measurements or single‐particle counting using resistance measurement (Coulter principle). Errors in measurements may result from various steps in sample handling, interaction of the sample with the environment and inborn errors of different techniques. High‐speed laser cytometry has been applied and was found to diminish the number of possible errors. The results of these measurements are compared with conventional techniq

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1979.tb02310.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY