摘要:

Abstract.The performance of two assays for the HBeAg/anti‐HBe system associated with hepatitis B virus infection, using monoclonal (EBK‐Sorin) and polyclonal (HBe‐Abbott) antibodies, has been compared. The results on a random population (1,000 samples) demonstrated for the monoclonal reagent a higher sensitivity, without any loss in specificity, and with the further advantage of the use of lower radioactivity levels. The proportion of positives and negatives obtained with the two kits was found to remain unchanged in the case of HBeAg, while a markedly larger percentage of positives (7% higher) was detected for anti‐HBe using the monoclonal ant

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1985.tb00159.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

Abstract.The function of four apheresis devices has been evaluated to obtain comparative data on performance and safety. Granulocyte yields were highest with the CS‐3000 with 1.7 times 1010cells, but this preparation also contained 4.5 times 1011platelets; plateletpheresis gave ranges of 3.4 times 1011to 4.5 times 1011cells. The amount of blood processed to obtain these yields varied greatly, ranging from 2.4 to 7 liters. Studies done on the donor return line showed no significant effects of the process. Comparative results on the efficacy of plasma exchange showed relatively little red cell contamination with any of these devices, but significant (6.9 times 1010) platelet removal in the Fenwal ba

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1985.tb00160.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

Abstract.The biochemical status of whole blood stored in containers fabricated of ethylene ethylacrylate (EEA) film was monitored at several times during 4 weeks of storage at 4°C. Fifteen biochemical indicators were studied to reflect on erythrocyte integrity, cellular metabolism, plasma protein stability, and microaggregate formation. Comparison to storage in polyvinyl chloride (PVC) containers was made by distributing aliquots from each unit of blood among the containers being compared. Whole blood in EEA developed significantly higher levels of plasma hemoglobin, erythrocyte osmotic fragility, andD‐glycerate‐2,3‐diphosphate (2,3‐DPG), and somewhat greater glucose utilization, lactate production, and pH. These biochemical differences were not of great magnitude and the data suggest that EEA containers are compatible with the storage of who

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1985.tb00161.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

Abstract.The red blood cells of blood stored in containers made of polyvinylchloride (PVC) film are osmotically more stable and lose on average about 1/3 less hemoglobin than when blood is stored in another plastic [poly‐(ethylene‐co‐ethyl acrylate); EEA]. The stability of uniform volumes of stored red blood cells varies directly with PVC surface area, whereas changes in EEA surface area have comparatively little or no effect. PVC contains high concentrations of the plasticizer, diethylhexylphthalate (DEHP), known to migrate into blood and to have a high potential for toxicity. To determine if DEHP could be the red cell stabilizing agent in PVC, whole blood was stored in containers made from EEA into which was incorporated varying amounts of DEHP. Incorporation of DEHP into EEA significantly reduced erythrocyte osmotic fragility (p = 0.01). The degree of reduced fragility correlated with the level of DEHP in the cell phase implicating DEHP in PVC containers as the stabilizing agent for red

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1985.tb00162.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

Abstract.Chloroquine‐treated platelets did not react with HLA antisera in a platelet suspension immunofluorescence test (PSIFT), while the reactions with platelet‐specific antibodies (PlAl, Baka) seemed even stronger after treatment. The method might be useful in discerning HLA and platelet‐specific antibodies without absorption procedures. The treatment causes death of a proportion of the cells, with nonspecific fluorescence making the reading difficult. This may be circumvented by using special phase‐contrast

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1985.tb00163.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

Abstract.Many examples of anti‐Rg (Rodgers) and anti‐Ch (Chido) have been studied by titration‐inhibition to assess their ability to detect partial inhibition (p.i.). Generally, anti‐Rg distinguishes the inhibition type Rg+ from Rg(+) (p.i.) and Rg–, whereas anti‐Ch distinguishes Ch– from Ch+ and Ch(+) (p.i.). Serological procedures for the detection of p.i. in random samples and the results of typing one family, apparently giving anomalous results,

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1985.tb00164.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

Abstract.Partial inhibition (p.i.) of anti‐Rg (Rodgers) and anti‐Ch (Chido) has been detected by antisera considered to be polyspecific. By using cells and C4 from individuals that are p.i. of Rg and Ch in absorption and elution experiments with selected antisera, it has been possible to separate several specificities. 2 Rg determinants, Rg1 and Rg2, and 3 Ch determinants, Ch1, Ch2, and Ch3, are defined by specific antisera. Partial inhibitors for Rg lack Rg2 and for Ch lack Ch2 or Ch3. The results are discussed in relation to other complexities of C4 polymorph

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1985.tb00165.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

Abstract.An inbred family A. G. with identical twin sisters having the Rhnullphenotype of the regulator type as confirmed by the maternal family data ofRhinheritance is reported. The family was traced back through church records for six generations. Several connections could be established with a Norwegian Sea Lapp community in which a family with the amorph Rhnulltype has been reported. The closeness of the Finnish and the Norwegian Lapps was given special attention since, despite both of the communities are small and share a more or less common gene pool, they nevertheless seem to possess the two ultra rareRhgenes,rin the Finnish and r in the Norwegian family, producing, when homozygous, the Rhnullphenotype.

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1985.tb00166.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

Abstract.Rh‐negative women, immunized to Rh by previous pregnancies, with only low concentrations of IgG anti‐Rh(D) in their plasma were assigned at random to test and control groups (7 subjects in each group). Both groups were challenged with an intravenous injection of 0.28 ml of Rh‐positive red cells; in addition, the test group received 500 μg anti‐Rh intramuscularly. 2 weeks after the injections, all subjects showed an increase in plasma anti‐Rh concentration; levels in test and control groups were similar. It is concluded that in Rh‐immunized subjects with low levels of IgG anti‐Rh a secondary response to Rh cannot be prevented by giving passively administered anti‐Rh wi

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1985.tb00167.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

Abstract.The cold agglutinin anti‐Li recognizes a sialic acid‐dependent antigen fully expressed only on newborn red cells. Although it resembles anti‐i in all aspects except for its nonreactivity with neuraminidase‐treated red cells, it is entirely different from anti‐i, since sialic acid is not involved in i antigenic det

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1985.tb00168.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY