摘要:

Abstract.A quantitative analysis was performed of the method of plasma fractionation used in our institute, a modified Cohn VI system, in order to develop and evaluate methods for the increment of antibody yield and quality of the immunoglobulin concentrate. For this purpose the distribution of the immunoglobulins IgG, IgM, IgA, the subclasses of IgG and several specific antibodies in the different fractions was analysed. In addition, the distribution of some plasma proteins which may be of importance for the quality of the immunoglobulin concentrate, such as α2M, α1‐antitrypsin, α2‐lipoprotein and fibrinogen, was determined. The average yield of IgG of immunoglobulin concentrates prepared from 31 plasma batches was 58% (SD 9%). Loss of IgG predominantly occurred in fraction III with an average of 25% (9 batches). Most of the measured antibody activities were similar to that of IgG. The anti‐D antibody showed a lower yield, the mean value of 13 batches was 41 % (SD 11 %), which could be attributed to a higher loss in fractions I and III.The steps in the ethanol fractionation in which considerable losses occurred, were analysed in order to improve the yield of immunoglobulins, especially of anti‐D. Suggestions for modifications leading to improvement of the yield

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1974.tb02409.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

Abstract.It was observed that during the preparation of normal anti‐D immunoglobulin concentrates by a modified Cohn VI method a considerable loss of anti‐D activity occurred in fraction III. A method was developed to prepare anti‐D immunoglobulin concentrates from fraction III by ethanol fractionation. Three batches were treated on a production scale. An average of 18% of the amount of anti‐D activity present in the original plasma, was recovered, which increased the total yield of anti‐D activity from 44 to 62%.The specific activity of anti‐D in the concentrate thus prepared was 2–3 times higher than that in the normal anti‐D immunoglobulin concentrate. The anti‐D immunoglobulin concentrate prepared from fraction III was diluted with normal immunoglobulin concentrate to reach an anti‐D concentration of 110–120 μg/ml.Using several analytical procedures no essential differences could be demonstrated between this final product and the normal immunoglobulin concentrate. After 8 months of storage at 4°C no significant decrease in anti‐D activ

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1974.tb02410.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

Abstract.Human factor II was isolated from plasma after absorption and elution of the prothrombin complex with barium citrate and ammonium sulphate, respectively. The eluate was brought onto a QAE Sephadex A‐50 column. The fractions containing coagulation factors were pooled, concentrated and chromatographed on hydroxyapatite, after which the fractions containing factor II activity were used for the immunization of rabbits. The antiserum produced is monospecific for factor II, as could be ascertained by immunoprecipitation and by inhibition of the coagulation activit

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1974.tb02411.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

Abstract.A method is described for obtaining hepatitis‐associated antigen (HAA) from the acid citrate dextrose plasma of routine blood donations found to be HAA‐positive. The separation consisted of the following steps: ammonium sulphate precipitation, absorption on and elution from DEAE‐Sephadex, and gel filtration on a Sepharose‐6B column. HAA was demonstrated by cross‐over immuno‐electrophoresis and lines of identity shown by the Ouchterlony double diffusion technique. Freedom from contaminating protein was shown by immuno‐electrophoresis. This material, when injected into rabbits, produced an anti‐HAA reagent suitable for use cross‐over immuno‐electrophoresis

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1974.tb02412.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

Abstract.Two patients with Rh mosaicism were studied, one of whom had a myeloproliferative syndrome. The red cell mixture was found to be composed of 30%DCe/dceand 70%dce/dcepopulations. The separated cell components did not differ in any other marker system and theirin vivosurvival as well as their glycolytic enzyme content were identical. Rh quantitative and kinetic assays did not provide any evidence for monosomy in D(‐) population. The data are discussed with reference to published cases of Rh mosaicism.Rh mosaics have always been a source of puzzling and stimulating problems. Raceand Sanger[17] found it curious that, up to 1964, all known propositi were found to live between 56° and 60 °N latitude. The purpose of this paper is to report on two new cases investigated in a lower latit

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1974.tb02413.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

Abstract.16 weak B60erythrocyte samples belonging to six families were studied using three series of quantitative measurements. The results show that the so‐called B60, group is heterogeneous and does not correspond to a genetic entit

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1974.tb02414.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

Abstract.Different concepts of cross‐reactivity are discussed in terms of a new descriptive system, model or conceptual framework which accounts for certain alleged quantitative and more or less continuous phenomena such as ‘reaction strength’ and ‘range of reactivity’ in discontinuous and qualitat

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1974.tb02415.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

Abstract.The authors suggest that the blood group antigens currently known as Sm and Buabe renamed Sc 1 and Sc 2 respectively, that the blood group system which they comprise be called Scianna and the locus controlling their production Sc. Linkage analysis by the lod score method rules out close linkage between Sc and ABO, Do, Fy, Jk, MN and P.

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1974.tb02416.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

Abstract.An additional case of a recombination in the HL‐A system involving HL‐A27 is reported and attention is drawn to the number of these in the total reported ca

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1974.tb02417.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

Abstract.Anti‐T agglutinin was studied in normal healthy subjects and in cirrhotic patients before and after oral administration of antibiotics depressing intestinal microbial flora. After antibiotic treatment anti‐T levels were significantly reduced. These observations show that bacterial antigens of intestinal origin are likely to be responsible for anti‐T production and that there must be common determinants between them and T antigen of red blood cells. It is not sure whether the previously reported increase of anti‐T levels in liver cirrhosis is in favour of the hypothesis that hypergammaglobulinaemia in this condition is due to failure of diseased liver to trap gut‐derived

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1974.tb02418.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY