摘要:

Abstract.Normal red blood cells (RBC) from fresh EDTA and CPD blood and from stored CPD blood were examined for the presence of bound subcomponents of C3 and C4. By serologic agglutination tests, only C3d was detectable on the cells. Incubation in compatible fresh normal serum (FNS) at 37 °C appeared to increase the amount of 3Cd on the RBC. C3b was serologically detectable only on stored CPD cells and only after incubation in compatible FNS. No C4 components were detected on the cell surfaces in agglutination tests. Using an indirect labeling technique, small, but significant, amounts of C3d and C4d were found on all three types of untreated cells. C3b was present on stored CPD cells only. The indirect labeling technique showed a significant increase in C3d and C4d on all cells following incubation in compatible FNS, whereas bound C3b was significantly increased only with stored CPD cells. There was no increase in bound C4b following serum incubation. The average number of C3d molecules per cell on normal EDTA cells was 557 and average KOwas 3.6 × 1071/mo

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1979.tb02261.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Abstract.Anti‐HBs, anti‐HBc and anti‐HBe were tested in blood donors negative for HBsAg by radio‐ and enzyme‐immunoassay. In this population 6.4% of the donors were positive for at least one type of antibody to HBV: 4.0% for anti‐HBs, 2.3% for anti‐HBc and 4.3% for anti‐HBe. Two sera (0.2%) were exclusively positive for anti‐HBc, eight sera (2.2%) were weakly positive only for anti‐HBe. Further studies of the infectivity of such samples m

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1979.tb02262.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Abstract.7 patients with primary humoral immunodeficiency were given an S‐sulfonated IgG preparation, 100 mg/kg i.v. at intervals of 3–4 weeks, for treatment of, or prophylaxis against, infection. The clinical effects and metabolism of S‐sulfonated IgG were studied. No side reactions attributable to S‐sulfonated IgG occurred in any of the patients. The S‐sulfonated IgG was completely transformed into intact IgG within 24 h after administration, and had a mean half‐life of 21 days, comparable to that of intact IgG. Complete restoration of IgG Fc fragment activity occurred within 24 h following injection, as assessed by reversed passive cutaneous

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1979.tb02263.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Abstract.A case of posttransfusional immunologic thrombocytopenia is reported in a 75‐year‐old PlA1‐negative woman. This was the second episode of postoperative and post‐transfusion thrombocytopenia in the same patient who had had only one pregnancy. Both thrombocytopenic episodes were subclinical and discovered by systematical hematologic study. A potent anti‐PlA1antibody (titer 1/128) was demonstrated by the platelet‐indirect radioactive Coombs test which appeared more sensitive than other platelet immunological assays used. The very long duration of the thrombocytopenia is discussed as well as the therapeutic possibilities in

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1979.tb02264.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Abstract.The P1and Pkblood group glycolipid antigens have the common terminal disaccharide, Gal(α, 1–4)Gal, but previous studies indicated that anti‐P1from P2individuals does not cross‐react with Pkantigen. In this paper, the specificities of anti‐P1and anti‐Pkwere analyzed carefully by complement fixation and hemagglutination techniques and the following results were obtained: (1) Anti‐P1from P2serum was not absorbed with the Pkglycolipid (CTH), but this antigen absorbed all anti‐P1and anti‐Pk(anti‐P1Pk) antibodies from the sera of four p individuals. Most of the anti‐P1Pkantibodies were IgG, but the anti‐P1from the P2individual was IgM. (2) The Pkantigen on normal P2erythrocytes was not ‘cryptic’. It was reactive with p serum from which the anti‐P antibodies were removed by absorption with the P glycolipid (globoside). This was not appreciated previously because, in order to make anti‐Pkreagents, p sera (anti‐P1PPk) were absorbed with P1cells which contain CTH. (3) The anti‐P1Pkantibodies in p sera were separated by partial absorption with P1erythrocytes and elution from the absorbing cells, into two fractions that differ markedly in their affinity for α‐methyl‐D‐galactoside a

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1979.tb02265.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Abstract.The red cell Kell phenotypes were examined in 15 Japanese patients with chronic granulomatous disease. No McLeod type was found. A low incidence of the association of chronic granulomatous disease with the McLeod type in the Japanese is suggested.

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1979.tb02266.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Abstract.The distribution of theFygene was studied in 1,207 Israeli Jews and 509 Arabs. The Fy(a‐b‐) phenotype(FyFy)was observed in Moslem, Christian and Druze Arabs, and in Jewish immigrants from Yemen and Iraq, but not in Sephardi or Ashkenazi Jews. TheFygene frequencies in Arabs and Jews were compatible with historical evidence of interactions with native African and admixed regional populations. Compared with Rho(cDe) and Jsa, Fy(a‐b‐) is a more useful genetic marker for recognizing African admixture in Middle Eastern popu

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1979.tb02267.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Abstract.The polymorphism of the second component of human complement was studied by means of isoelectric focusing in polyacrylamide gels with subsequent complement‐dependent lysis of sensitized sheep erythrocytes in an agarose overlay containing C2‐deficient or normal human serum. In a material of 289 unrelated individuals the following gene frequencies were observed:C2l= 0.965 andC22= 0.035. The rare phenotype C2 2 (= C2 B) could be seen once in a child of a C2 1–2 heterozygous mother. The investigation of the C2/HLA relationship revealed a very close linkage: Among 62 informative meiotic divisions one recombination between HLA‐B and C2 was found (i.e. 1.61%); in addition,C22was significantly associated withHLA‐B15and‐Cw3.In a family with an HLA‐A/C crossover C2 segregated together with HLA‐C, in a family with an HLA‐B/D(DR) crossover C2 segregated together with HLA‐D(DR). This supports the assumption of a C2 structural locus outside HLA‐B, pr

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1979.tb02268.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Abstract.These data comprise 1,231,024 routine tests carried out over a 5‐year period on voluntary blood donors. The percentage of positive results on the machines varies from 1 to 3% of the total number of samples tested. Antibodies identified either by manual or automated techniques make up 15–20% of the positive screening reactions. Rhesus, Lewis and P systems prevail, whilst Duffy, Kidd and Lutheran systems are absent.This screening has three main advantages: the supply of plasma for our production of test sera, or therapeutic immunoglobulins; protection of the recipient; partial information on the donor's immunohaematological state, especially for the risk of giving him incompatible blood sometimes in the fut

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1979.tb02269.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1979.tb02270.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY