摘要:

Abstract.Kinetic studies have revealed the direct solid phase radioimmunoassay for hepatitis B antigen (Ausria, Abbott Laboratories) to be markedly sensitive to incubation temperature. The present 18‐hour room temperature incubation can be reduced to 2 h at 50 °C without loss in test sensitivity. The rapid test is also considerably more specific than that carried out under standard conditions.If licensed, this modification would permit HB Ag screening by RIA to be carried out on labile blood produc

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1974.tb02689.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

Abstract.The size distribution of particles of purified hepatitis B antigen (HB Ag) was altered by the action of complement. Prior to exposure to complement 94% of round particles of HB Ag were in a size distribution of 10–18 nm with a modal diameter of 15 nm. The modal diameter of particles treated with complement increased to 19 nm and particles larger than 30 nm were prominent. This effect on the morphology of HB Ag may be due to an enzymatic action of complemen

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1974.tb02690.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

Abstract.An I‐active substance was isolated from the aqueous layer obtained after extraction of human erythrocyte membranes withn‐butanol. This substance was found to be identical with the antigens with A, B, and H blood group specificity which we had isolated previously. Thus, all A, B, H and I blood group activity in aqueous extracts of erythrocyte membranes is dependent upon the same kind of antigenic material which may carry different blood group specificities. The material is sialic acid free and comprises about 90% carbohydr

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1974.tb02691.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

Abstract.The authors describe an automatic method of determining the percentage of erythrocyte agglutination with regard to reference sera. By use a of continuous flow system, it has been possible to adapt this method in population genetics to a considerable number of subjects. The correlation of the automatic with the classical manual method is good. Its adaptation to other techniques of quantitative immunology appears feasible.

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1974.tb02692.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

Abstract.A healthy Caucasian woman who possessed blood groups A2H(O) on her red cells as well as in her saliva and serum had no anti‐blood group B ‘complete’ or ‘incomplete’ agglutinins nor hemolysins as determined either on whole serum or its concentrated β‐γ‐globulin fraction. No anti‐B antibody was stimulated by immunization with highly active human blood group B glycoprotein.No blood group B antigenic determinants were demonstrable on the proposita's red cells or in her saliva by any of numerous serological and enzymatic methods at various red cell concentrations between 2 and 37 °C, nor by the highly sensitive immunoglobulin‐latex test. Immunization with the proposita's red cells did not elicit anti‐blood group B antibodies in chickens or rabbits. Blood groups A and O but not B occurred in members of this woman's family which was studied

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1974.tb02693.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

Abstract.A family is described in which three members are of the recessive Lu(a–b–) type. These individuals also lack the high‐frequency Lutheran‐related antigens Lu4, Lu5, Lu6, Lu7, Lu8, Lu11, Lu12 and Lu13. There is no evidence that lack of Lutheran antigens compromises red‐cell membrane

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1974.tb02694.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

Abstract.The determination of the alanin aminotransferase activity in serum is useful as part of the health control of blood donors and is a valuable supplement to the tests for hepatitis B antigen and antibody.

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1974.tb02695.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1974.tb02696.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

Abstract.Qualitative changes were found to occur in the reaction of HBAg with HBAb in the course of viral hepatitis. These variables were found to depend on what was defined as the ‘quantity’ and ‘reactivity’

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1974.tb02697.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1974.tb02698.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY