摘要:

Abstract.Protein blotting is a technique of transferring and immobilizing the original protein patterns from gel matrices to a suitable solid phase. It can be employed in identifying the protein antigens in complex biological mixtures, since each protein is accessible to its corresponding antibody. This method, called immunoblotting, is also applicable to define the specificity of a given antibody. The perspective of immunoblotting has already found wide application in basic research. Some features of the immunoblotting and recent improvements of the original procedures will be discussed. To justify a wider application in clinical research I will summarize some applications with respect to immunohematology.

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1986.tb04863.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1986.tb04864.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Abstract.A selected group of women previously immunized in pregnancy by the Rh(D) antigen were boosted with 0.5 ml of Rh(D)‐positive frozen recovered red cells from an accredited donor panel. The response was prompt and sustained and increased the anti‐D content by almost 10‐fold. In spite of the reduced rate of clinical immunisation to the Rh(D) antigen, it was possible to recruit females with anti‐D, acquired in pregnancy, for secondary boosting and a programme is recommended which maximizes efficiency and

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1986.tb04865.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Abstract.We extended the time of keeping whole blood at 20–24 °C to 15 h (overnight) after phlebotomy for preparing platelet concentrates. We have evaluated the in vitro characteristics of platelets and blood cells prepared from whole blood drawn into CPD‐AD, an anticoagulant containing 0.4 mMadenine and 1.5 times more dextrose than CPD. We studied in vitro red cell and platelet function of blood cooled either within 4 h after collection or after a 15‐hour delay. In vitro platelet function measured as hypotonic shock reaction, aggregation response to ADP and collagen and14C‐serotonin uptake were not significantly different after preparation and after a 5‐day storage period. Units held at room temperature for 15 h after blood collection exhibited a level of 2,3‐DPG that was 45% of that exhibited by red cells held for 15 h at 1–6 °C. All other in vitro parameters of red cell concentrates measured during 35 days of storage were not significantly different. Based on these in vitro data blood drawn into CPD‐AD might be kept up to 15 h at room temperature prior to refrigeration in order to prepare plat

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1986.tb04866.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Abstract.Isopycnic density floatation centrifugation has been proven to be a suitable technique to enrich bone marrow aspirates for clonogenic cells on a small scale. We have tested a Haemonetics semicontinuous blood cell separator in order to process large volumes of bone marrow with minimal bone marrow manipulation. The efficacy of isopycnic density floatation was tested in a one and a two‐step procedure. Both procedures showed a recovery of about 20% of the nucleated cells and 1–2% of the erythrocytes. The enrichment of clonogenic cells in the one‐step procedure appeared superior to the two‐step enrichment, first separating buffy coat cells. The recovery of clonogenic cells was 70 and 50%, respectively. Repopulation capacity of the low‐density cell fraction containing the clonogenic cells was excellent after autologous reinfusion (6 cases) and allogeneic bone marrow transplantation (3 cases). Fast enrichment of large volumes of bone marrow aspirates with low‐density cells containing the clonogenic cells by isopycnic density floatation centrifugation can be done safely using a Haemonetics blood cel

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1986.tb04867.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Abstract.1,860 serum samples of blood donors from northern Nigeria were tested for the presence of the hepatitis B surface antigen. 8.9% of them were found to be positive. Significant differences in frequency were observed amongst various ethnic groups as well as ABO blood groups.

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1986.tb04868.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Abstract.We have described the use of a monoclonal125I‐labeled anti‐IgG (125I‐MA) to assay IgG antibody displayed on the surface of platelets from normal and immune thrombocytopenic patients and reported levels of IgG 10–100‐fold lower than previous studies. This report describes the immunologic characteristics of the125I‐MA and the assay for surface IgG. The125I‐MA has a high binding affinity for surface‐displayed IgG (2.22 times 109M‐1), reacts equally well with all four subclasses of IgG and not at all with IgM or IgA. In our assay, the binding ofI25I‐MA was found to be ≥99% specific for IgG (no nonspecific association ofI25I‐MA with platelets) and the binding ratio ofI25I‐MA to IgG displayed on the cell surface was 0.91 (close to unity). Finally, platelet lysates were found to contain large amounts of IgG protein (39,597 ± 27,418 molecules/platelet) as compared to surface‐displayed IgG (124 ± 86 molecules/platelet). This assay has excellent characteristics for quantitation of IgG on platelets and the discrepancy with other techniques may, in part, be due to intentional or inadvertent lysis of plat

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1986.tb04869.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Abstract.Hybridomas secreting a human monoclonal IgG1antibody against a variant of the rhGantigen were produced from B lymphocytes of an Rh‐negative donor with serum antibodies to D and C antigens. The antibody reacts by indirect hemagglutination with nearly all C‐ or D‐positive cells, confirming the strong association in the expression of G with D or C antigens. The lack of antibody reactivity to C‐negative Ducells suggests a particular epitope on the D complex associated with the G

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1986.tb04870.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Abstract.Utilizing a kinetic enzyme‐linked antiglobulin binding assay, eight examples of anti‐Leawere detected in individuals whose Lewis phenotype is Le(a‐b+). These antibodies were not detectable by routine hemagglutination techniques, but were indistinguishable from anti‐Leamade by Le(a‐b‐) individuals in terms of their immunologic fine specificity. However, unlike most anti‐Leaantibodies from Le(a‐b‐) individuals, none of the anti‐Leaantibodies had an IgG component. Anti‐Leaantibodies in Le(a‐b+) individuals may represent immune responses on the continuum between autoim

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1986.tb04871.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Abstract.Two anti‐Leasera which were able to detect Leaantigen on platelets were identified in a screening for anti‐platelet antibodies by means of a platelet complement fixation test. These two antisera hemolyzed erythrocytes without enzyme treatment. The anti‐Leaactivity could be completely absorbed by red cells, platelets and lymphocytes of Le(a+b‐) donors but not by cells from Le(a‐b+) or Le(a‐b‐) donors. The antibody activity against red cells was eliminated by treatment of the antisera with dithiothreitol, thereby suggesting that the activity resided in the IgM class of immunoglobulins. As the anti‐Leawas more reactive at 37°C than at room temperature against both red cells and platelets, we suggest that transfusion of platelets of Lea‐negative donors should be considered for patients with thi

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1986.tb04872.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY