1. |
Lack of Conformity in the Behaviour of Platelets during Normal Storage Conditions at 22°C |
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Vox Sanguinis,
Volume 40,
Issue 2,
1981,
Page 65-70
Lars Eriksson,
Claes F. Högman,
Christer Busch,
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摘要:
Abstract.Platelets prepared from citrate‐phosphate‐dextrose blood under normal routine conditions and concentrated to 1,000–1,600 · 109· 1‐1varied considerably in their capacity to acidify the medium. Serotonin uptake was well maintained for 5 days provided that adenosine 5′‐triphosphate was within 50% of normal and pH was above 6.0. A strong release of platelet factor 4 was occasionally seen in relation to preparation. In some preparations the platelet factor 4 was well maintained for 5 days, in others a sudden or gradual release was seen during storage. The investigation indicates that differences in the quality of routinely prepared platelets occur during preparation and storage which are insufficiently well explained a
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1981.tb00671.x
出版商:Blackwell Publishing Ltd
年代:1981
数据来源: WILEY
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2. |
Glycoprotein‐Sialyltransferase Activity of Normal Human, Thrombasthenic and Bernard‐Soulier Platelets |
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Vox Sanguinis,
Volume 40,
Issue 2,
1981,
Page 71-78
Brigitte Bauvois,
Jean‐Pierre Cartron,
Alan Nurden,
Jacques Caen,
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摘要:
Abstract.Some properties of the sialyltransferase activity of homogenates prepared from normal human platelets were investigated using asialo‐fetuin as substrate. The enzyme activity was optimal at pH 6.5 and was stimulated by divalent cations in the order Mg2+>Mn2+>Ca2+. Buffers of high ionic strength strongly reduced the activity. ATP and ADP were not inhibitors at 0.1 mMconcentration, but AMP, CTP and CMP reduced the activity by 15–30%. A native endogenous acceptor for the enzyme activity was located in the platelet homogenates. The range of fetuin‐sialyltransferase activity found in platelets isolated from 6 normal donors was 79 ± 39 pmol/h/mg protein (mean±SD). The platelets of patients with Glanzmann's thrombasthenia and the Bernard‐Soulier syndrome, which are characterized by different membrane glycoprotein deficiencies, were shown to have fetuinsialyltransferase activities within the normal range indicating that the membrane glycoprotein defects in the platelets of these patients are not associated with the absence of sialyltransferase
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1981.tb00672.x
出版商:Blackwell Publishing Ltd
年代:1981
数据来源: WILEY
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3. |
Anti‐Nomifensine Antibody Causing Immune Hemolytic Anemia and Renal Failure |
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Vox Sanguinis,
Volume 40,
Issue 2,
1981,
Page 79-84
B. Habibi,
J. P. Cartron,
M. Bretagne,
P. Rouger,
C. Salmon,
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摘要:
Abstract.An anti‐Nomifensine antibody was identified in a patient with immune hemolysis and renal failure who was under Nomifensine therapy. The anti‐drug specificity of the antibody was confirmed by inhibition assays using Sepharose gel‐Nomifensine conjugates. Preliminary screening tests for anti‐Nomifensine antibody in a population of 104 patients treated by the drug showed no further example of such immun
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1981.tb00673.x
出版商:Blackwell Publishing Ltd
年代:1981
数据来源: WILEY
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4. |
Red‐Cell Enzyme Activities and Properties of Mutant Pyruvate Kinase after 1‐Year Storage in a ‐80°C Freezer |
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Vox Sanguinis,
Volume 40,
Issue 2,
1981,
Page 85-90
Yoji Ishida,
Shiro Miwa,
Susumu Takegawa,
Noboru Fujinami,
Kunio Yamato,
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摘要:
Abstract.Red cells from 5 healthy adults and an unstable mutant pyruvate kinase (PK), PK Maebashi, were stored for 12 months in a ‐80°C freezer. With an exception of triosephosphate isomerase and phosphoglycerate kinase activities, normal red‐cell enzyme activities remained essentially unchanged. Mutant PK was characterized by the methods recommended by the International Committee for Standardization in Haematology (ICSH). Normal control and PK Maebashi remained essentially unchanged in their characteristics after storage in a ‐80°C freezer for 12 months. These results indicate that the red cells stored over a long term in a ‐80°C freezer can be used for the study of unstable
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1981.tb00674.x
出版商:Blackwell Publishing Ltd
年代:1981
数据来源: WILEY
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5. |
Follow‐Up Studies of Healthy Blood Donors. Anti‐HBs Antibody Carriers |
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Vox Sanguinis,
Volume 40,
Issue 2,
1981,
Page 91-94
J. Ślusarczyk,
J. Cianciara,
H. Świderska,
T. Łoch,
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摘要:
Abstract.10 healthy blood donors persistently seropositive for anti‐HBs and without a history of clinically overt viral hepatitis were observed for periods of time ranging from 46 to 57 months. Physical examinations and biochemical liver function tests were normal in all cases. Immunologic studies of their immune response to hepatitis B virus antigens are suggestive for a late period of convalescence from clinically inapparent hepatitis
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1981.tb00675.x
出版商:Blackwell Publishing Ltd
年代:1981
数据来源: WILEY
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6. |
Significance of Antibody to Hepatitis Be Antigen (Anti‐HBe) in HBsAg‐Negative Individuals |
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Vox Sanguinis,
Volume 40,
Issue 2,
1981,
Page 95-98
G. Hess,
W. Arnold,
G.M. Gahl,
E. Vogl,
K.H. Meyer zum Büschenfelde,
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摘要:
Abstract.A study was initiated to assess the significance of antibody to hepatitis B e antigen (anti‐HBe) in HBsAg‐negative individuals. Anti‐HBe was demonstrated in the majority of sera positive for anti‐HBs and anti‐HBc. All sera positive for anti‐HBs and negative for anti‐HBc and most sera positive for anti‐HBc but negative for anti‐HBs were anti‐HBe‐negative. This implies that the antibody response to HBeAg is of shorter duration than that to HBsAg and HBcAg. Anti‐HBe may help to discriminate between various states of hepatitis B virus infection found to be associated with anti‐HBc‐positive but HBsAg‐ and anti‐HBs‐negative sera. 2 individuals were anti‐HBe‐positive but HBsAg‐, anti‐HBs‐ and anti
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1981.tb00676.x
出版商:Blackwell Publishing Ltd
年代:1981
数据来源: WILEY
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7. |
Monoclonal Anti‐B as a New Blood‐Typing Reagent |
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Vox Sanguinis,
Volume 40,
Issue 2,
1981,
Page 99-104
S. H. Sacks,
E. S. Lennox,
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摘要:
Abstract.Stable cloned lines of anti‐B‐secreting cells have been derived by fusing spleen cells of a mouse immunized by group B antigen with a mouse myeloma line. The tissue culture supernatant of one of them (NBI/19.112.28) containing secreted monoclonal anti‐B antibody is an especially good blood‐grouping reagent, well suited for use with manual and automated methods. The unlimited availability of a potent reagent of uniform properties, cheaply produced by a single source, makes monoclonal anti‐B a serious competitor to human typ
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1981.tb00677.x
出版商:Blackwell Publishing Ltd
年代:1981
数据来源: WILEY
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8. |
Intravascular Hemolytic Transfusion Reaction due to Anti‐Vw+Miawith Fatal Outcome |
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Vox Sanguinis,
Volume 40,
Issue 2,
1981,
Page 105-108
Lyndall Molthan,
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摘要:
Abstract.Because of the increasing use of type, screen and hold protocols and minimal surgical blood order protocols in transfusion services, a number of patients are receiving un‐cross‐matched blood in elective situations. There are many low‐frequency red blood cell antigens which are lacking on reagent cells used for antibody screening procedures, and alloantibodies directed against these antigens are relatively common and occur in either natural or immune forms. A case of an intravascular hemolytic transfusion reaction resulting in death is reported. The patient had a naturally occurring anti‐Vw+Miaand received 1U of Vw+Mia‐positive donor red cells. It is the 1st documented case of a hemolytic transfusion reaction due to this incompatibility. The potential threat of transfusion reactions due to low‐frequency antigens must be recognized by the physicians who design type, screen and hold protocols and it has particular reference to the selection of possible recipients for whom the protocols
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1981.tb00678.x
出版商:Blackwell Publishing Ltd
年代:1981
数据来源: WILEY
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9. |
Studies on Ultrafiltration of Antihemophilic Factor |
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Vox Sanguinis,
Volume 40,
Issue 2,
1981,
Page 109-114
Gautam Mitra,
John L. Lundblad,
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摘要:
Abstract.Ultrafiltration as an alternative approach to reprecipitation was studied in the production of an intermediate‐purity antihemophilic factor concentrate suitable for clinical use. Almost complete recoveries of factor‐VIII‐related activities have been demonstrated across the processing step resulting in increased net yield of the product. Several important processing parameters are discussed. The product thus prepared is stable and has improved VIII:C/mg fibrinogen
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1981.tb00679.x
出版商:Blackwell Publishing Ltd
年代:1981
数据来源: WILEY
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10. |
Which are the Parameters to be Controlled in Platelet Concentrates in Order that They May be Offered to the Medical Profession as a Standardized Product with Specific Properties? |
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Vox Sanguinis,
Volume 40,
Issue 2,
1981,
Page 115-126
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ISSN:0042-9007
DOI:10.1111/j.1423-0410.1981.tb00680.x
出版商:Blackwell Publishing Ltd
年代:1981
数据来源: WILEY
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