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1. |
Detection of Hepatitis B Antigen m Blood Donors by Radioimmunoassay and Three Reverse Passive Haemagglutination Techniques |
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Vox Sanguinis,
Volume 31,
Issue 2,
1976,
Page 81-86
S. Seidl,
G. B. Ziegler,
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摘要:
Abstract.In a comparative study 5,569 sera from voluntary donors were tested simultaneously for hepatitis B antigen by three reverse passive haemagglutination (RPHA) techniques (Auscell®, Hepanosticon®, Hepatest®) and the radioimmunoassay (RIA) (Ausria II). Among the three RPHA methods, no significant differences could be found as far as sensitivity is concerned. The percentage of positive sera detected by these RPHA methods varies from 0.43 to 0.56%. Eleven sera were detectable only by Ausria II. Except for two, all these sera had low counts per minute in the R
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1976.tb02192.x
出版商:Blackwell Publishing Ltd
年代:1976
数据来源: WILEY
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2. |
Influence of Counting Statistics on Borderline Detection of Hepatitis B Antigen by a Solid‐Phase Radioimmunoassay |
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Vox Sanguinis,
Volume 31,
Issue 2,
1976,
Page 87-95
A. L. Wagner,
J. M. Lemaire,
D. R. Richard,
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摘要:
Abstract.The detection of hepatitis B antigen by a solid‐phase radioimmunoassay technique is by now well established, particularly where transfusions are concerned. However, it would appear that the use of systematic procedures leading to positive detection, such as the ‘cutoff’ method (mean counts per minute of the negative controls multiplied by a coefficient of 2.1), are open to criticism if elementary precautions concerning the time span and the statistical accuracy of radioactivity counting are not taken. The method may even prove inaccurate in a certain number of borderline cases studied in relation to plasma and different sera, especially if subjected to a single screening process over a large number of samples analyzed by computer. These deficiencies can be avoided by correcting the positive coefficient in relation to the mean counts per minute of negative controls, the statistical accuracy (relative standard deviation) and the distribution of counting
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1976.tb02193.x
出版商:Blackwell Publishing Ltd
年代:1976
数据来源: WILEY
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3. |
Linkage of Gene for C2 Deficiency and the Major Histocompatibility Complex MHC in Man Family Study of a Further Case1 |
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Vox Sanguinis,
Volume 31,
Issue 2,
1976,
Page 96-102
Noorbibi K. Day,
P. Rubinstein,
D. Case,
J. A. Hansen,
R. A. Good,
M. E. Walker,
N. Tulchin,
B. Dupont,
C. Jersild,
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摘要:
Abstract.Close linkage between HL‐A and C2 deficiency was first reported by Fu and co‐workers in 1974. We present here a pedigree of a 31‐year‐old C2‐deficient individual with clinical manifestations of Hodgkins disease. The following markers were tested: C2 levels, factor B polymorphism, blood groups, and enzyme typing. In addition to close linkage between HL‐A and C2 deficiency, both parents were heterozygous for Bf (HL‐A linked, electrophoretic variation of B). The two HL‐A haplotypes closely linked to C2 deficiency are different: 2,W18 and W24,W18. They share, however, the SD2 antigen W18 and
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1976.tb02194.x
出版商:Blackwell Publishing Ltd
年代:1976
数据来源: WILEY
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4. |
An Unusual Rh Phenotype Indicating Heterogeneity of the CwAntigen |
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Vox Sanguinis,
Volume 31,
Issue 2,
1976,
Page 103-108
B. Habibi,
J. André,
M. T. Fouillade,
M. Lopez,
C. Salmon,
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摘要:
Abstract.A family is reported in which a new Cwantigen occurred in two generations. This was recognized by 17 anti‐Cwsera, but by none of the 21 anti C sera which were, however, shown to react strongly with common Cw+ cells. This unusual finding provides evidence that the Cwantigen is in fact heterogeneous. On the basis of data obtained from absorption‐elution and coagglutination studies a tentative explanation is attempted: common Cw+ phenotypes are assumed to be Cw(+1+2) and the present phenotype Cw(+1–2). Anti‐Cwsera should accordingly be anti‐Cw1, whereas anti‐C sera should only rea
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1976.tb02195.x
出版商:Blackwell Publishing Ltd
年代:1976
数据来源: WILEY
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5. |
Hemolytic Disease of the Newborn due to Anti‐N |
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Vox Sanguinis,
Volume 31,
Issue 2,
1976,
Page 109-116
M. TelischiDirector,
Oscar Behzad,
Peter D. Issitt,
Beverly G. Pavone,
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摘要:
Abstract.A Negro woman of phenotype M+, N‐, S‐, s‐, U+, who produced anti‐N, is described. The antibody in her serum caused hemolytic disease of the newborn in her M+, N+
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1976.tb02196.x
出版商:Blackwell Publishing Ltd
年代:1976
数据来源: WILEY
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6. |
Differentiation of Human Leukemic from Normal Lymphocytes by Limulns Serum Agglutination |
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Vox Sanguinis,
Volume 31,
Issue 2,
1976,
Page 117-123
E. Cohen,
J. Minowada,
M. Pliss,
L. Pliss,
L. E. Blumenson,
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摘要:
Abstract.Human peripheral chronic lymphocytic leukemic lymphocytes and a line (B411‐4) of cultured human B cells are agglutinated byLimulusserum to a significantly higher titer and score than peripheral normal human lymphocytes or a line (MOLT‐4‐F) of cultured human T
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1976.tb02197.x
出版商:Blackwell Publishing Ltd
年代:1976
数据来源: WILEY
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7. |
Observations of the Number of Available G (rhG, Rh 12) Antigen Sites on Red Cells1 |
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Vox Sanguinis,
Volume 31,
Issue 2,
1976,
Page 124-130
F. Skov,
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摘要:
Abstract.The number of available G antigen sites within the rhesus system were estimated using trace‐labelled antibody. The results given as available antigen sites_ per cell were as follows: R1R1: 9,900‐12,200; r'r': 8,200‐9,700; ryr: 8,600; RNaN: 6,600; r‘xr: 6,200; RB1r: 6,200; RZRZ: 5,400; Cdes: 4,800‐5,100; R2R2: 3,600‐5,800; R0R0: 4,500‐5,300; R2r 4,200; r“Gr: 1,700‐3,600; RB2r: 1,400‐1,900. The G antigen is detected in optimal amount on C‐positive samples. When D is present without C, the number of G sites is apparently dependent on the expression of all the D antigens within the recognized six cat
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1976.tb02198.x
出版商:Blackwell Publishing Ltd
年代:1976
数据来源: WILEY
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8. |
Anti‐Diaand the DiaBlood Group |
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Vox Sanguinis,
Volume 31,
Issue 2,
1976,
Page 131-135
W. Graninger,
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摘要:
Abstract.In an Austrian family the Diaantigen was discovered by causing hemolytic disease of the newborn. No mongoloid admixture has so far been detected. No linkage to other blood groups, serum groups or red cell enzymes could be found.
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1976.tb02199.x
出版商:Blackwell Publishing Ltd
年代:1976
数据来源: WILEY
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9. |
Incidence of Viral Hepatitis after Administration of Factor IX Concentrates |
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Vox Sanguinis,
Volume 31,
Issue 2,
1976,
Page 136-140
Sten Iwarson,
Hans Kjellman,
Ann‐Catrine Teger‐Nilsson,
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摘要:
Abstract.A high frequency of viral hepatitis has been reported after treatment with the human factor IX concentrate ‘Konyne’. Clinical trials with ‘Konyne’ and a similar factor IX concentrate, called ‘Preconativ’, was started in Sweden 1969. During the first 2 years, 26 patients were treated with either one or both preparations. Nine patients developed viral hepatitis within 6 months after treatment. ‘Preconativ’ alone was introduced on the Swedish market in 1971. During the period 1971–1974, another 26 hemophiliacs were treated but only two cases of hepatitis have occurred. Selection of donors and screening for hepatitis B surface antigen in donor blood used for the manufacturing of ‘Preconativ’, might be contributing factors to this lo
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1976.tb02200.x
出版商:Blackwell Publishing Ltd
年代:1976
数据来源: WILEY
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10. |
Alternatives for Plasma Fractionation1 |
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Vox Sanguinis,
Volume 31,
Issue 2,
1976,
Page 141-151
W. Schneider,
D. Wolter,
L. J. McCarty,
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摘要:
Abstract.At the present time there is an enormously increasing demand for albumin. The most common procedure for the isolation of this plasma component is the cold ethanol technique developed by Cohn. Because this process necessarily isolates other blood components for which there is less demand in relation to albumin, albumin production is expensive. Therefore, we have developed a two‐step fractionation for the isolation of albumin. It is basically a heat precipitation method with the albumin yield being about 90% of the original plasma albumin. In comparison to cold ethanol methods, it is considerably less expensive. Other blood components, e.g., clotting factors, immunoglobulins, may also be isolated. A non‐modified γ‐globulin for intravenous use is obtained by removing anti‐complementary activity with hydroxyethyl starch. Additional fractionation steps are required to isolate these other components, but unlike in established methods, these are not necessary for the isolation of solely
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1976.tb02201.x
出版商:Blackwell Publishing Ltd
年代:1976
数据来源: WILEY
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