摘要:

Abstract.Buffy coat granulocyte concentrates were prepared manually from single fresh whole blood donations after centrifugation. The erythrocyte, granulocyte and platelet numbers in these concentrates were compared to cell numbers in concentrates obtained by mechanical leukapheresis using intermittent flow centrifugation. Buffy coats contained approximately half the number of granulocytes and three times the number of erythrocytes per unit volume than cell separator concentrates. There was no difference in platelet numbers. Thus to give a therapeutic dose of granulocytes equivalent to that obtained by cell separation, 2–3 times greater volume of buffy coat concentrates would be required.In vitro granulocyte function, as measured by migration, phagocytosis, and Candida killing was also compared in buffy coat and cell separator concentrates. The only difference found was reduced Candida killing in the buffy coat granulocytes.The possible clinical use of buffy coat concentrates, as a source of granulocytes, is discusse

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1981.tb01003.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Abstract.Packed erythrocytes which are white cell and microaggregate poor and virtually platelet free, with a long shelf life (35 days), can be prepared from a routinely used plastic pack system incorporating a cotton wool filter module. The filtered packed cells have improved physical and biochemical properties after prolonged storage, and with acceptable autologous erythrocytic survivals the system lends itself to routine clinical practice. It avoids the need for microaggregate filters at administration, at the same time reducing the immunogenicity of the transfused blood. Blood component harvesting is not compromised.

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1981.tb01004.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Abstract.Units of CPDA‐1 whole blood were subdivided and each treated with additions of dihydroxyacetone (DHA) to give final concentrations from 0 to 80mM. The ‘optimum’ concentration of DHA to maintain 2,3‐diphosphoglycerate (2,3‐DPG) with minimal loss of ATP during 42 days of storage appeared to be 30mMof DHA. With this formulation, red cell 2, 3‐DPG concentrations rose to 130–140% of normal by 14 days and then decreased in a near‐linear manner to 50–60% normal by 42 days, while maintaining adequate ATP levels. In addition, packed red cells were prepared from CPD fresh blood and treated with adenine, glucose, and various concentrations (0‐80mMof DHA. The cells also responded most favorably to 30mMDHA, although the response was not as positive as whole blood. This concentration of DHA produced nearly 100% maintenance of 2,3‐DPG at 14 days with subsequent fall to 30%

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1981.tb01005.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Abstract.A dynamic hemolysis curve was examined on stored ACD blood by a new instrument (mean cell volume or MCV continuous analyzer) during treatment with either saponin or with hypotonic medium. Several changes of the dynamic hemolysis curves were recorded during storage. With saponin hemolysis, these changes appeared earlier and were more marked than those of hypotonic hemolysis.

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1981.tb01006.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Abstract.We have developed a mixed passive haemagglutination test for the detection of platelet antibodies. This test is essentially a combination of a modified test of mixed agglutination and reversed passive haemagglutination. Using this test, platelet allo‐antibodies (HLA antibodies and non‐HLA antibodies) were found to be detected with very high sensitivity in the sera of pregnant women and transfused patients. The platelet crossmatch test was performed for patients who had to receive massive platelet transfusion. There was a good correlation between the result of the crossmatch and clinical platelet recov

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1981.tb01007.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Abstract.We report a new case of immune haemolytic anaemia induced by dipyrone. The haemolytic crisis was followed by acute renal failure. The direct antiglobulin test on the red cells was positive. The responsible antibody was of the IgG class; it was complement binding and produced haemolysis in vitro. The clinical and serological findings suggest that the red cells became sensitized as the result of the adsorption to the cell membrane of drug‐antibody immune complexe

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1981.tb01008.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Abstract.A case of hemolytic disease of the newborn due to anti‐Lebis described. The mother possessed high titer IgG anti‐Lebreacting at 37°C and complement binding in nature. The infant had mild hemolytic disease with a positive direct Coombs' test using anti‐IgG and anticomplement. Anti‐Lebcould be eluted from the infant's r

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1981.tb01009.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Abstract.A Jr(a‐) Japanese female developed anti‐Jraduring her first pregnancy. Both father and infant red cells were Jr(a+), and anti‐Jrawas eluted from the infant's red cells. The antibody was determined to be IgGl. Hemolysis could not be definitively established from the clinical data. The pitfall of using the presence of jaundice as the sole evidence for hemolysis is emphasized. We conclude that the present case, and other previously reported cases, do not unequivocally establish that anti‐Jracauses significant hemolytic diesease of the newborn (HDN). Amniocentesis probably should not be performed during the pregnancy of mothers sensitized to Jraantigen. JraHDN is probably a mild disease, like

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1981.tb01010.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Abstract.Haemolytic disease of the newborn due to rhesus anti‐ē alone is a rare occurrence. This condition is described in the second child of an R2R2mother who had not previously been transfused. The antibody was of IgG subclasses IgG1+IgG3 and was detectable on the baby's cells for 4 months after birth. The anaemia was mild and persisted for the same peri

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1981.tb01011.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

Abstract.A new low incidence red cell antigen, Van Vugt (Vga) has been found in a blood donor. A family study showed that the antigen is inherited as a Mendelian autosomal dominant character. Vgasegregates independently from the MNSs blood group system. Only one example of the antigen was found on testing 17,209 blood donors. Anti‐Vgais not uncommon in sera containing multiple antibodies to low frequency antigen

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1981.tb01012.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY