摘要:

Abstract.Spleens from 14 healthy adult cats were isolated and perfused with cell‐free Ringer's solution. Histological studies on the splenic megakaryocytes, as well as a search for splenic megakaryocytes in samples of the splenic venous outflow, were performed. The samples of the splenic venous outflow contained a lower cellular concentration when perfusion was prolonged, but the number of megakaryocytes appearing in the venous samples increased. At least five different forms of megakaryocytes were observed from smears of the venous outflow: (i) unipolar, (ii) multipolar, (iii) foamy, (iv) mobile and (v) nuclear types. These types were also found in splenic venous outflow of dogs, kittens and bled cats. It is suggested that not only platelets but also the larger megakaryocytes can pass out of the splenic circulation. This may imply that megakaryocytes can be culled, matured in the splenic red pulp, and ultimately released into peripheral bloo

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1978.tb02909.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

Abstract.The characteristics of an immunohematologic reaction to isoniazid are described with emphasis on the mechanism of development of the positive antiglobulin test. In contrast to previous findings, the antibody is a firmly bound IgG complement‐binding antibody, reacting best at 37°C by antiglobulin techniques and only with isoniazid presensitized cells. A method for preparing isoniazid‐sensitized cells is descr

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1978.tb02910.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

Abstract.A large, 81 member, four‐generation black family with hereditary angioedema is reported with regards to its clinical course and the association with the histocompatibility system. No mortality was seen related directly to the disease. Assuming that this trait is autosomal dominant it appeared to have no linkage with the histocompatibility system as noted by an estimated recombination rate in males and females of 0.5 and a maximum lod score of 0.0. Further evidence of no linkage is given by the fact that lod scores below 3.0 were observed for values of 0 less than 0.

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1978.tb02911.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

Abstract.In this paper we describe a new sensitive solid phase radioimmunoassay for the detection of anti‐HBc. This test was shown to be more sensitive than the widely used immune adherence hemagglutination test (IAHA) and at least as sensitive as the radioimmunoassay using the blocking principle. The new test system appears to be very useful to screen larger groups of individuals (e.g. blood donors) for the presence of anti‐

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1978.tb02912.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

Abstract.Hepatitis B surface antigen (HBs Ag) and associated particles, e antigen (e Ag) and DNA polymerase are unevenly distributed during Cohn's cold ethanol fractionation of plasmas positive for these markers of the hepatitis B virus (HBV). Most of the e Ag, Dane particles and DNA polymerase are retained in fraction III whereas the bulk of HBs Ag is recovered in fraction IV where only 22 nm spheres and short filaments are still identified. These results suggest that differences in quantitative distribution of HB virions together with alteration of infectious particles during the fractionation process may in addition to heat inactivation account for the relative hepatitis risk of the various plasma derivatives.

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1978.tb02913.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

Abstract.In a patient of subgroup A2the serum contained an unusually potent anti‐A1, giving the following reactions with A1red cellsin vitro:agglutination of saline‐suspended cells up to a temperature of 32°C; a positive indirect antiglobulin test (complement only) at 37°C and lysis of enzyme‐treated cells at 37°C. A series of tests was carried out to estimate the ability of the antibody to destroy varying amounts of A1red cellsin vivo. When about 0.55 ml of red cells was injected, about 65% of the cells were destroyed within 30 min; 2 days later when 18.9 ml of cells were injected, only about 45% were destroyed within 30 min; 5 days after this when a whole unit of A1red cells was transfused, survival at 24 h was about 90%. This last figure may indicate that destruction of red cells by anti‐A1was negligible since at the time of the transfusion of the whole unit the patient was bleeding into her gastrointestinal tract. On the other hand, the titre of anti‐A1appeared to be declining spontaneously during the period in which tests were carried out so that, if the whole unit of A1blood had been transfused at the beginning of this period, survival might have been less good. Nevertheless, from the observed difference in survival between the 0.55 ml and 18.9 ml doses it seems safe to conclude that, even if the unit had been transfused at the time when the antibody concentration was maximal, the percentage of cells destroyed would hav

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1978.tb02914.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

Abstract.The qualitative and quantitative determinations of A, B, and H antigens were studied in blood samples of 509 normal subjects and 114 patients. 11 of 53 (20.75%), 3 of 27 (11.11 %), and 4 of 34 (11.76%) of adults with leukaemia, children with leukaemia and adults with lymphomas, respectively, show significantly lower antigenic scores than normal controls. Since all A, B and H antigens were found to be independently affected, the destruction of well‐developed ABH antigens by a certain substance produced during the course of the disease was suspecte

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1978.tb02915.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

Abstract.The erythrocytes of a normal man were agglutinated more weakly than normal cells by several anti‐P1PPksera, and exhibited a decreased capacity to absorb these antibodies. Analysis of his erythrocyte glycosphingolipids revealed that the globoside (P antigen) content was less than 25% of normal, and trihexosyl ceramide (the Pkantigen) was 30–40% of normal. The ganglioside content of his erythrocytes was approximately four times normal and sialosyl‐paragloboside was increased about sixfold. It appears that his erythrocytes are unable to synthesize normal quantities of trihexosyl ceramide, and that these serological and chemical features constitute a new phenotype in the P blood group s

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1978.tb02916.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

Abstract.Several examples of Ohcells have been investigated with various anti‐H reagents. After enzyme treatment of the cells with either papain or neuraminidase, very high titres were obtained, particularly with some preparations ofUlex europeus. Fractionation of anti‐HUlexshowed that the anti‐H component inhibitable byL‐fucose required papain treatment of the Ohcells for optimal activity whereas the component not inhibitable byL‐fucose agglutinated preferentially Ohcells treated with neuraminidase. The anti‐H lectinCytisus sessifoliuswhich is not inhibitable byL‐fucose gave no reaction with papain‐treated Ohcells but reacted well when the Ohcells were treated with neuraminidase. Normal O cells were almost equally well agglutinated by the lectins when treated by either of the two enzymes. That the various lectin components activated by enzymes had H specificity was shown by the fact that their activity was inhibited by purified H substance, partially inhibited byL‐fucose and 2‐fucosyllactose and not at all by purified Leasubstance and non‐secretor salivas.In spite of the high titres obtained with Ohcells by the enzyme technique, the amount of H present on the red cells, judged by inhibition tests, in comparison with normal group O

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1978.tb02917.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

Abstract.A serologically typical group A2B mother with no unusual anti‐A or anti‐B agglutinins delivered a normal group O infant with no demonstrable A or B antigens. By the age of 1 year the child had developed anti‐A and anti‐B agglutinins. He had H antigen and secreted H substance in his saliva. A second group Ochild has since been born. Although family studies were inconclusive, the most likely explanation is that the mother is acis‐AB. If this is the case, she differs from other reportedcis‐ABs in her apparently normal B antigen and the lack of anti‐B antibodies

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1978.tb02918.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY