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1. |
Demonstration of HL‐A Antigens on Human Kidney Cells by Means of the Micro‐Immune Adherence Assay |
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Vox Sanguinis,
Volume 24,
Issue 4,
1973,
Page 289-300
J. P. H. B. Sybesma,
B. A. Planque,
Tjitske Soelen,
Else Borst‐Eilers,
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摘要:
Abstract.The presence of HL‐A antigens on human kidney cells was investigated by means of the micro‐immune adherence assay (MIAA). The MIAA was performed on kidney cells obtained from fresh kidneys and from kidneys stored at ‐96°C, on kidney cells maintained in culture, and on kidney cells stored in liquid nitrogen after programmed freezing.Performed on fresh kidney cells, the MIAA has a reproducibility of 96–97%. Performed on cells from traditionally frozen kidneys, on cells maintained in culture, and on cells kept in liquid nitrogen after programmed freezing, the assay shows poor reproducibility and non‐specific adherence occurs. This might partly explain the differences observed by other investigators in typing and cross‐matching with lymphocytes on the one hand, and kidney cells on the other; for in these procedures kidney cells, usually from a tissue culture, are typed by means of the MIAA. Modern long‐term kidney preserving techniques make it possible to perform the MIAA on fresh kidney cells for typing and cross‐matching in kidney
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1973.tb02646.x
出版商:Blackwell Publishing Ltd
年代:1973
数据来源: WILEY
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2. |
Low Protein Rh Immune Globulin (Rh IgG) |
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Vox Sanguinis,
Volume 24,
Issue 4,
1973,
Page 301-316
J. M. Bowman,
B. Chown,
J. Pollock,
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摘要:
Abstract.After 16–32 months in solution at 4°C, ultracentrifuge and immunoelectrophoresis assays of five lots of low protein Rh IgG protein concentrations 3.9, 5.8, 4.1, 3.4 and 3.1%, revealed each lot to consist of a single unaggregated and unfragmented protein with ans20wconstant of 6.5–7.3. The lots were 94.0–98.5% pure IgG. Autoanalyzer studies demonstrated no loss of anti‐D for 16–37 months except in one lot stable at 20 months which showed a drop from 363 to 298 μg/ml at 26 months.125I antiglobulin measurements revealed a 23‐ to 40‐percent early loss of anti‐D with stability thereafter. Low protein Rh IgG cleared Rh‐positive cells from 11 Rh‐negative volunteers and protected 2,093 Rh‐negative women at risk of Rh immunization at least as effectively as standard Rh IgG. Low protein Rh IgG is a pure, stable, active, protective and economical agent fo
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1973.tb02647.x
出版商:Blackwell Publishing Ltd
年代:1973
数据来源: WILEY
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3. |
Electrophoretic Precipitation and Radial Diffusion Methods for Assay of Anti‐D Immunoglobulin Preparations |
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Vox Sanguinis,
Volume 24,
Issue 4,
1973,
Page 317-322
N. C. Hughes‐Jones,
Brigitte Gardner,
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摘要:
Abstract.The measurement of anti‐D concentration has been carried out by two immunoprecipitation techniques utilizing sonicated D‐antigen‐containing stroma suspended in agarose. One method utilizes single radial immunodiffusion; in the other method, anti‐D is moved into the agarose by electrophoresis. The results obtained form both immunoprecipitation techniques were compared to those obtained by a standard125I‐antiglobulin method. The coefficient of variation of the difference between the results obtained by immunoprecipitation and those obtained from the125I‐antiglobulin method were ±14% for the electrophoretic method and ±16% for the radial dif
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1973.tb02648.x
出版商:Blackwell Publishing Ltd
年代:1973
数据来源: WILEY
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4. |
Characterization of Rh Antibodies Formed after Incompatible Pregnancies and after Repeated Booster Injections |
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Vox Sanguinis,
Volume 24,
Issue 4,
1973,
Page 323-330
A. Morell,
F. Skvaril,
J. L. Rufener,
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摘要:
Abstract.Indirect hemagglutination studies were performed on 10 incomplete anti‐Rh sera from mothers after incompatible pregnancies, on 19 incomplete anti‐Rh plasma samples from preimmunized and repeatedly boosted individuals and on 3 anti‐Rh plasma pools. Group O Rh‐positive red blood cells were agglutinated by antisera to immunoglobulins. Anti‐Rh antibodies after incompatible pregnancies were found to belong to the immunoglobulin class IgG and to the subclasses IgG1, IgG3 and IgG4 only. In most anti‐Rh samples from preimmunized and repeatedly boosted subjects, IgG and IgA were present. In six anti‐Rh samples of this group, the subclass IgG2 could be
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1973.tb02649.x
出版商:Blackwell Publishing Ltd
年代:1973
数据来源: WILEY
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5. |
Autoimmune Haemolytic Anaemia due to Red Cell Antibodies of Different Specificities in a Patient with Chronic Hepatitis |
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Vox Sanguinis,
Volume 24,
Issue 4,
1973,
Page 331-336
Andrea M. Lightwood,
G. L. Scott,
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摘要:
Abstract.A patient with chronic hepatitis with associated autoimmune phenomena developed red cell antibodies with specificities in the I blood group system and against stored red cells. These antibodies were all IgM in type and causedin vivohaemolysis which responded to treatment with corticosteroids.
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1973.tb02650.x
出版商:Blackwell Publishing Ltd
年代:1973
数据来源: WILEY
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6. |
A New Anti‐Erythrocyte Antibody in the Duffy System: Anti‐Fy4 |
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Vox Sanguinis,
Volume 24,
Issue 4,
1973,
Page 337-342
O. Behzad,
C. L. Lee,
J. Gavin,
W. L. Marsh,
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摘要:
Abstract.A new Duffy antibody, designated anti‐Fy4, was found to react with all samples of type Fy(a‐b‐), some of type Fy(a‐b+) and Fy(a+b‐), and none of type
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1973.tb02651.x
出版商:Blackwell Publishing Ltd
年代:1973
数据来源: WILEY
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7. |
Further Studies of Xh, a Serum Protein Antigen in Man1 |
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Vox Sanguinis,
Volume 24,
Issue 4,
1973,
Page 343-353
Georgia M. Dunston,
H. Gershowitz,
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摘要:
Abstract.Xh has been identified as a serum protein antigen whose expression is both age and sex dependent, and is shown here to be identical with the antigen previously reported as Pa 1. On immunodiffusion, approximately 75% of randomly selected sera from females of all ages form a precipitin line with specific rabbit antiserum. Frequency of the antigen in females is correlated with age; 94% over 19 years of age are positive while only 55% under 19 are positive. Approximately 47% of male sera are positive; no significant changes in frequency associated with age of males were found. The mean level of the antigen in the sera of females over 19 years of age is approximately twice that in male sera. The heritability of Xh expression on the basis of a study of male twins was 0.66.A study of 88 families, with 181 offspring selected so that female offspring were at least 20 years old, was inconclusive in two ways: (1) two female offspring exceptional to the hypothesis of sex linkage were found and (2) no negative by negative matings were observed. Thus, although the Xh data are suggestive of a genetic polymorphic system, the influence of physiological factors on Xh expression and the findings, reported here, of similarly high frequencies in adult females of all racial groups, make it more probable that this antigen is an isotypic marker.Reactivity with anti‐Xh was found among sera of some of the great apes and Old and New World monkeys but not among prosimians and lesser mammals. The isotypic specificity is presumed to have arisen during the speciation process leading to the present day anthropoid apes and ma
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1973.tb02652.x
出版商:Blackwell Publishing Ltd
年代:1973
数据来源: WILEY
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8. |
Serological Studies on the Pr1/Pr2Antigens using Dog Erythrocytes. |
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Vox Sanguinis,
Volume 24,
Issue 4,
1973,
Page 354-361
D. Roelcke,
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摘要:
Abstract.A heterogeneity of the Pr1antigen is demonstrated using dog erythrocytes: Pr1h/Pr1d. Pr1his human erythrocyte‐specific while Pr1dis also found on dog erythrocytes. Though also the Pr2antigen is found on dog red cells, it is independent of Pr1d. Pr2is demonstrated on dog erythrocytes also after papainization while Pr1dis only found on untreated dog erythrocytes. The parallel of Pr1hantigen deficiency and Pr2antigen increase on dog red cells in comparison with human red cells to human erythrocyte glycoproteins after periodate oxidation in comparison with untreated human red cell glycoproteins is stressed out and discusse
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1973.tb02653.x
出版商:Blackwell Publishing Ltd
年代:1973
数据来源: WILEY
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9. |
The Stability of the I, HI, and H Blood Group Antigens During Storage at 4°C1 |
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Vox Sanguinis,
Volume 24,
Issue 4,
1973,
Page 362-365
R. M. Lambert,
J. P. Downing,
S. K. Zelenski,
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摘要:
Abstract.In agglutination experiments using antisera of human origin, the I, HI and H erythrocyte antigens were shown to be quite stable on red cells that had been collected as clotted blood and in ACD‐B solution and maintained at 4°C for periods as long as 28 days. These blood group antigens were also well preserved on red cells that had been frozen at ‐150°C in the vapor phase of liquid nitrogen and, after recovery from the frozen state, were maintained at 4°C in a dextrose‐electrolyte solution fo
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1973.tb02654.x
出版商:Blackwell Publishing Ltd
年代:1973
数据来源: WILEY
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10. |
Plurality of Determinants of the Australia Antigen |
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Vox Sanguinis,
Volume 24,
Issue 4,
1973,
Page 366-371
B. Babudieri,
G. D. Rottini,
R. Favento,
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摘要:
Abstract.By means of the absorption method and complement fixation test it has been possible to identify six different determinants of the Au antigen and to establish that there is no determinant common to all Au‐positive ser
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1973.tb02655.x
出版商:Blackwell Publishing Ltd
年代:1973
数据来源: WILEY
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