ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1991.tb00940.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Abstract.The effect of storage of platelets in a new polyvinyl chloride (PVC) plastic material with a butyryl‐n‐trihexyl citrate (BTHC) plasticizer (PL 2209) was evaluated. The PL 1240 container, i.e. PVC plastic with a different plasticizer, tri‐(ethylhexyl)‐tri‐mellitate, was used as a reference. Measurements of pH, pO2, pCO2, glucose, lactate, adenosine triphosphate, total adenine nucleotide content, lactate dehydrogenase and platelet factor 4 (PF4) were made during 5 days of storage. Similar results were noted comparing PL 2209 and PL 1240. Differences in pO2and pCO2indicate greater gas permeability in PL 2209 than in PL 1240. Significantly higher PF4 levels were found in PL 2209, but the difference could not be attributed to the PL 2209 container itself. Paired autologous reinfusion studies (111Indium) of 6 normal donors gave mean recovery values after 5‐day storage of 41.1 ± 7.4% (PL 2209) and 45.5 ± 7.7% (PL 1240),t1/266 ± 13 and 75 ± 5 h, survival time (linear model) 6.3 ± 1.0 and 6.8 ± 0.7 and survival time (multiple‐hit model) 6.0 ± 0.7 and 6.5 ± 0.4 days, respectively. Only the difference in survival time (multiple‐hit) was significantly higher in PL 1240. The corrected count increments at 12–24 h following transfusion were 13,300 ± 10,800 (PL 2209) and 13,600 ± 11,600 (PL 1240) with no statistically significant difference found. These results indicate PL 2209 as an equivalent alternative to PL 1240 for the

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1991.tb00941.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Abstract.Follow‐up studies on 67 blood donors with indeterminant serological findings for human T‐lymphotropic virus (HTLV) type I by standard immunoassays showed no evidence of infection by polymerase chain reaction analysis for HTLV‐I or HTLV‐II nucleic acids or by antibody reactivity to a unique HTLV‐I recombinant envelope protein, MTA‐4. Among HTLV‐I‐ or ‐II‐infected individuals, a history of blood transfusion, past residence in established HTLV‐I endemic areas or some association with intravenous drug use were common. In contrast, 85% of indeterminant cases had none of these risk factors. These observations suggest that healthy individuals with indeterminant serology for HTLV‐I should not req

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1991.tb00942.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Abstract.A comparison between recombinant immunoblot assay hepatitis C virus (HCV) first generation (RIBA‐1) and second generation (RIBA‐2) was made on 732 blood donors reactive by anti‐HCV ELISA (Ortho Diagnostics System) by the Hepatitis Study Group of the French Society of Blood Transfusion. RIBA‐2 results were correlated with ELISA ratio and ALT levels. The number of both reactive and nonreactive samples was higher with RIBA‐2 than with RIBA‐1, 252 (34%) compared to 224 (31%) for reactive samples, and 404 (55%) compared to 307 (42%) for nonreactive samples. C 22–3 and C 33‐c reactivities were observed in 96 and 91% of the reactive samples, respectively. A total of 76 samples (11%) remained indeterminate by RIBA‐2, 84% of them reacting only on C 100–3 antigen. A clear relationship between RIBA‐2 results and both ELISA ratio and alanine aminotransferase (ALT) levels was demonstrated: 20% of samples with normal ALT level and 10% of samples with low ELISA ratio were reactive when 91% of samples with ALT>2N and 69% of samples with high ELISA ratio were reactive. The totality of the 57 samples with both ALT>2N and high ELISA ratio were reactive and 93% of samples with normal ALT level and low ELISA r

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1991.tb00943.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Abstract.The value of intravenous immunoglobulin (IVIG) in the treatment of 24 severely Rh‐sensitized pregnant women was studied. IVIG was infused at a daily dose of 0.4 g/kg maternal body weight for 4–5 consecutive days, and was administered again 15–21 days later until delivery, depending on the evolution of the hemolytic disease. Our population was divided into 3 groups according to the time of onset of therapy: group 1 (n = 8), before 20 weeks' gestation; group 2 (n = 7), 20–28 weeks, and group 3 (n = 9), after 28 weeks. Initial mean anti‐D level was significantly higher in group 1 (25.9 ± 12.9 IU/ml) than in the other 2 groups, whose mean values were, however, higher than 10 IU/ml. Amniotic‐fluid total bilirubin levels before the onset of therapy were pathologic, and in 55% of the cases they coincided with zone 3 of Liley's chart. Hydrops fetalis at the onset of treatment accounted for the only 3 fetal deaths in groups 1 and 2. None of the fetuses developed hydrops during treatment. Six of the 9 neonates in group 3 were depressed at birth (1‐min Apgar below 7). However, at 5 min only 1 newborn showed an Apgar below 7. Mean birth weight was over 2,500 g in all the cases. Neonatal hematological condition in group 2 (50% of the babies required only phototherapy) was better than in the other 2 groups (transfusional therapy). There was a significant fall in maternal anti‐D titers and intrauterine hemolysis after IVIG treatment. In order to further evaluate the response to IVIG therapy, the 13 cases in our population with a history of fetal/neonatal death in the previous pregnancy were analyzed separately. The results obtained in this particular group of patients demonstrated again the effectiveness of IVIG treatment.Conclusions: There were no adverse effects on the mother or fetus, and there was no need for neonatal intensive care. Results were more favorable if treatment was started before 28 weeks' gestation in non

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1991.tb00944.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Abstract.The clinical course and laboratory findings of a patient with autoimmune haemolytic anaemia due to anti‐phospholipid antibodies and secondary to a systemic lupus erythematosus‐like syndrome are described. IgG and IgM with anti‐phospholipid activity coexisted in both sera and acid eluates prepared from the patient's red cells, as demonstrated by ELISA using a panel of anionic and neutral phospholipids. The anti‐erythrocyte binding activity of eluted autoantibodies, determined by a radioactive antiglobulin technique, was totally inhibited by absorption with phospholipid micelles. The patient's serum also contained an IgM warm haemolysin detectable with enzyme‐treated cells only, as well as anti‐C, ‐E and ‐S

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1991.tb00945.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Abstract.The specific aim of this study was to characterize human anti‐Rh monoclonal antibodies cross‐reacting with self‐antigens. We studied supernatants from man‐mouse hybridomas and from lymphoblastoid cell lines. Man‐mouse hybridomas were established by fusion of peripheral blood lymphocytes from healthy individuals recently immunized against Rh alloantigens, with mouse myeloma (or man‐mouse heteromyeloma) cell lines. Lymphoblastoid cell lines were produced by Epstein‐Barr virus induction of lymphocytes from identical sources. Of the 55 monoclonal alloantibodies studied, 11 also reacted with intracellular self‐antigens as demonstrated by immunofluorescence assay on cryostat sections of human tissues. This cross‐reactivity was mainly a property of monoclonal alloantibodies belonging to the IgM isotype (among the 11 cross‐reacting mAbs 10 were IgM). The cross‐reactivities of these monoclonal antibodies were ascertained by absorption of alloreacting antibodies with red blood cells. Similar results were obtained on a panel of purified cellular antigens by ELISA. The results confirm that during an immune response against a foreign antigen (alloantigen), B cells that produce polyreactive antibodies are not excluded from the pool of responding cells. Therefore, polyreactive autoantibodies present in sera from healthy individuals may be the result of an immune response aga

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1991.tb00946.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Abstract.The expression of Lewis antigens is thought to be controlled by the Secretor and Lewis genes. While secretor status is known to regulate the expression of ABH antigens in many tissues, few studies have attempted to correlate Lewis antigen expression on erythrocytes and saliva with that of epithelial tissues. We examined the expression of Lewis a and b and related antigens in human epithelium of minor salivary glands and labial nonkeratinized oral mucosae from 16 individuals by immunohistology using monoclonal antibodies. The expression of these antigens, as detected by monoclonal antibodies (MAbs) used, was correlated with erythrocyte phenotype and saliva secretor status. In acinar cells of glands, Lebantigen was expressed only in secretors, and Leaonly in nonsecretors. However, in gland ducts and oral mucosae, Lebwas found in both secretors and nonsecretors, as well as in 2 cases of Lea‐b‐, secretors. Thus, antigen expression in acinar cells of minor salivary glands was correlated with the predicted genotypes, whereas inappropriate expression of Lebantigen was found in epithelial cells of gland ducts and oral mucosae. The present data indicate that the Lewis blood group phenotype is regulated differently in duct cells and stratified epithelium than in saliva and erythrocy

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1991.tb00947.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1991.tb00948.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1991.tb00949.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY