摘要:

Abstract.In 30 granulocyte donors undergoing one or more infusions of 6% hydroxyethyl starch (HES) solution plasma HES concentrations were measured by single radial immunodiffusion. Serial HES applications led to a cumulative increase in plasma HES levels. Up to 3 months after the last HES infusion, plasma concentrations in the range of 3 mg/ml were demonstrable. A possible influence of HES deposits upon reticuloendothelial system function is discussed.

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1980.tb01855.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Abstract.An H‐deficient blood is described which, unlike other H‐deficient, Bombay and para‐Bombay bloods, has normalHtransferase levels. In previous examples of Bombay and para‐Bombay phenotypes the absence ofHtransferase has explained the lack of H substrate and therefore the weak A and B antigens which are characteristic of these phenotypes. This explanation obviously does not hold in the case described here and it is proposed that the biochemical block must be further back, either in the enzymes synthesising the oligosaccharide chains or in those making the GDP‐fucose substrate for theHand theLegene‐specified fucosyltransferases. Inhibition of the synthesis of Lebsubstance, as well as H substance, although the saliva of the propositus contains theLegene‐specified transferase, supports thi

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1980.tb01856.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Abstract.Red blood cells (RBC) were collected with citrate‐phosphate‐dextrose (CPD) in a blood‐pack optimal additive system. After concentration to 90% hematocrit they were diluted with saline‐adenine‐glucose medium (SAG‐RBC), and stored for 35 days. In this work the RBC were stored in the presence of leukocytes. The SAG medium allows RBC conservation during 35 days at +4°C. The adenosine triphosphate (ATP) level of RBC is compatible with their survival. During the first 2 weeks, hemolysis of SAG‐RBC was not greater than in CPD blood. Nevertheless, hemolysis reached 1.49% on day 35, and there was a marked increase in RBC osmotic fragility. Scanning electron‐microscopic studies of 35‐day RBC showed that the majority of them became echinocytes. After incubation in fresh frozen plasma, the RBC recovered satisfactory osmotic resistance and normal disc shape. The post‐transfusion viability was normal with>70% recovery after 48 h. Thein vivorestoration of 2,3‐diphosphoglycerate (2,3‐DPG) was rapid in the transfused SAG‐RBC, 50% of the initial 2,3‐DPG level being restored in 1 h. Thein vivostudies proved that the functional quality of these RBC was compatible with their use in transfusion. The most important problem concerns the supernatant hemoglobin level of the SAG‐RBC to b

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1980.tb01857.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Abstract.An agglutinin was identified in the serum of a non‐transfused primiparous patient that reacted with all commercial red blood cells regardless of their antigenic makeup. The autologous control was negative. The agglutinin was directed against human red blood cells that had been incubated in media containing glucose and washed prior to testing. Red blood cells incubated in 2% glucose solution became agglutinable after 6 days at 4°C or after 2h at 37°C. These red blood cells gradually became non‐reactive when washed and incubated in saline at 37 or 4°C. At 22°C the titer of the agglutinin was 128 (score 64) in LISS and 64 (score 56) in saline. The agglutinin was denatured by 2‐mercaptoethanol, but remained active after 30 min of heating at 56°C. Among various mono‐ and disaccharides tested, glucose was the only sugar that selectively inhibited the reaction. The presence of such an agglutinin may cause confusion in a transfusion service laboratory by mimicking an antibody directed against a high incid

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1980.tb01858.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Abstract.We here report 2 patients who have acquired two consecutive episodes each of acute hepatitis, within 6 months after blood transfusion. The clinical and biochemical course was typical of recurrent acute viral hepatitis, and the patients recovered completely after each episode. The first infection was probably caused by non‐A, non‐R hepatitis virus, whereas the second episode was caused by hepatitis B‐virus. Thus, in addition to the absence of cross‐immunity between hepatotrophic viruses, one may simultaneously acquire more than one virus without apparent inter

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1980.tb01859.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Abstract.A reverse passive hemagglutination (RPHA‐G) technique for the automated screening of hepatitis B surface antigen (HBsAg) on the Groupamatic 360 (Kontron International, Zürich, Switzerland) has been developed. It is more sensitive than manual RPHA (RPHA‐M) and nearly as sensitive as radioimmunoassay (RIA); it detects 6 ng/ml of HBsAg, compared to 3 ng/ml by RIA and 25 ng/ml by RPHA‐M. Although it is slightly inferior to the other two methods in respect of specificity, the incidence of false‐positive reactions was only 0.34%. The test can also be performed manually without the Gro

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1980.tb01860.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Abstract.The serum of an elderly man, group A1Le(a+b−), contained an IgM antibody that agglutinated his own cells and the cells of random group A1donors. Over a period of 5 months, the titre of these auto‐anti‐A1agglutinins was 4 at

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1980.tb01861.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Abstract.Two Bx (a+) families are described, andBxais shown to segregate independently from several genetic markers. Anti‐Bxahas so far only been detected as an antibody without an identifiable stimulu

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1980.tb01862.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1980.tb01863.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1980.tb01864.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY