ISSN:0749-503X    

DOI:10.1002/yea.320090802

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

摘要:

AbstractSaccharomyces cerevisiaeHSc was grown with ethanol at concentrations up to 10% (v/v). The immediate effects of additions of externally added ethanol on CO2production and O2consumption of washed organisms were studied by stopped‐flow membrane inlet quadrupole mass spectrometry. Fermentative activities of organisms grown with ethanol (0–5% v/v) showed similar sensitivities to inhibition by ethanol, whereas those grown with 10% (v/v) ethanol had become protected and were markedly less sensitive.The fluidity of subcellular membrane fractions was measured by determination of the temperature dependence of the rotational order parameter of the spin label 5‐doxyl stearic acid (free radical) by electron spin resonance. Mitochondria prepared from yeasts grown with 0, 7 and 9% (v/v) ethanol showed similar overall fluidity, although differences in temperature‐dependent behaviour indicate altered lipid composition or lateral phase separations. On the other hand the microsomal fraction from organisms grown with 9% ethanol showed a remarkable increase in fluidity. These data suggest that the protective effects of growth with ethanol near the limit of tolerance on fermentative activities may arise from altered plasma membrane fluidity pro

ISSN:0749-503X    

DOI:10.1002/yea.320090803

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

摘要:

AbstractInduced production of arginase (CAR1) enzyme activity and steady‐stateCAR1mRNA inSaccharomyces cerevisiaerequires wild‐typeARG80/ARGRIandARG81/ARGRIIgene products. We demonstrate here that these gene products, along with that of theMCM1gene, are required for the inducer‐dependentUASI‐A, UASI‐BandUASI‐Celements to function but they are not required for operation of inducer‐independentCAR1 UASC1orUASC2M. Through the use of single and multiple point mutations, theCAR1 UASI‐BandUASI‐Celements were demonstrated to be at least 23 bp in length. Moreover, simultaneous mutation of both ends of an elements gave stronger phenotypes than mutations at either end. The center of the element was more sensitive to mutation t

ISSN:0749-503X    

DOI:10.1002/yea.320090804

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

摘要:

AbstractThe abundant DNA‐binding proteins ABF1 and CPF1 are members of a family of global regulators with diverse chromosomal functions in the yeastSaccharomyces cerevisiae. Recent evidence suggests that these protein factors may be involved in establishing and maintaining well‐defined chromatin structures in promoter regions and other genetic elements. We have investigated the involvement of ABF1 and CPF1 in chromatin organization at theQCR8gene, encoding subunit VIII of the mitochondrial ubiquinol‐cytochromecoxidoreductase. The promoter region of theQCR8gene contains overlapping binding sites for ABF1 and CPF1. Nucleosome positioning studies indicate that theQCR8gene is associated with a phased array of nucleosomes under both catabolite‐repressed and derepressed growth conditions. Analysis of binding site mutants reveals that both ABF1 and CPF1 are involved in maintaining a nuclease‐hypersensitive region in theQCR8promoter. The chromatin structure atQCR8during steady‐state growth is, however, mainly dependent on binding of ABF1 to the promoter region. Implications of these findings for the role played by ABF1 and CPF1 in the regulation of mitochondrial biogenesis and other processes important for cell growth and division will b

ISSN:0749-503X    

DOI:10.1002/yea.320090805

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

摘要:

AbstractAn upshift of the growth temperature from 26 to 40°C in the presence of calcium leads to the aggregation ofKluyveromyces marxianuscells and to the formation of flocs. Analysis of cell wall proteins, either by sodium dodecyl sulphate–polyacrylamide gel electrophoresis of extractable mannoproteins or by immunolocalization, revealed an accumulation of a protein with Mr 37 kDa (p37), upon flocculation. Immunological studies confirmed the homology of this protein with the glycolytic enzyme glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH). When mRNA isolated from cells growing at 40°C was translatedin vitro, a 35 kDa newly labelled protein was synthesized and immunoprecipitation assays showed that this protein is recognized by p37‐antiserum, suggesting that the 35 kDa polypeptide might be an unglycosylated precursor form of p37. The results indicated that the presence of this cell wall mannoprotein closely related to GAPDH is dependent on the growth temperature, suggesting its role a

ISSN:0749-503X    

DOI:10.1002/yea.320090806

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

摘要:

AbstractThe metabolic effects of the administration of fructose to a yeast expressing the cDNA for rat liver ketohexokinase have been investigated by31P‐nuclear magnetic resonance spectroscopy. Cessation of growth suffered by the yeast on exposure to 5 and 25 mM‐fructose was accompanied by a large accumulation of fructose 1‐phosphate at the expense of cytoplasmic orthophosphate and nucleoside triphosphate. Shifts in resonances were consistent with a drop in cytoplasmic pH. Arresting growth with 1 mM‐fructose, however, did not result in these changes, although a large accumulation of fructose 1‐phosphate occurred which may have been supported by the mobilization of polyp

ISSN:0749-503X    

DOI:10.1002/yea.320090807

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

摘要:

AbstractTheSEC18gene product is required for protein transport at different stages in theSaccharomyces cerevisiaesecretory pathway. The homologousSEC18gene fromCandida albicanshas been cloned by complementation of asec18‐1 S. cerevisiaethermosensitive mutant using aC. albicansgenomic library in YRp7. Sequence analysis of the gene revealed a 2382‐bp open reading frame which coded for a protein of 88 926 kDa. By anin vitrotranscription–translation coupled reaction of theC. albicans SEC18gene, a protein of approximately 85 kDa was obtained. Hydrophobicity analysis of the protein did not show any predicted signal sequence nor transmembrane anchor domain. These results and the fact that glycosylation was absent in the protein indicated thatC. albicansSec18p did not enter in the secretory pathway. The alignment of the amino acid sequence revealed that theSEC18gene fromC. albicanswas homologous to theSEC18fromS. cerevisiae(50% amino acid identity) and to the gene that coded the N‐ethylmaleimide‐sensitive factor (NSF) protein (43% amino acid identity). Moreover, theC. albicansSec18p also showed the putative ATP binding site present inS. cerevisiaeSec18p a

ISSN:0749-503X    

DOI:10.1002/yea.320090808

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

摘要:

AbstractThe analysis of theSaccharomyces cerevisiaechromosome III DNA sequence by computer (‘in silico’) permits the definition of its linguistic characteristics. These characteristics include the designation of non‐randomly occurring oligonucleotides, their distribution along the chromosome, and the distribution of some particular homopolymers. All these elements may contribute to the understanding of the organization of information on the chrom

ISSN:0749-503X    

DOI:10.1002/yea.320090809

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

摘要:

AbstractWe isolated a mutant defective in C‐terminal farnesyl cysteine:carboxyl methyltransferase activity from a screen for mutations causinga‐specific sterility. A genomic fragment was cloned from a yeast multi‐copy library that restored mating. Both the cloned gene and the sterile mutation were allelic to theSTE14gene. Aste14‐complementing 2·17 kbBamHI fragment subclone was sequenced and found to encode a 239 amino acid protein with a molecular weight of 27,887 Daltons. The hydrophobicity profile of the methyltransferase reveals the presence of at least five potential transmembrane domains. In comparisons of the C‐terminal methyltransferase amino acid sequence with those in the PIR and Swiss protein databases, no significantly similar sequences were found nor were conserved regions from other methyltransferas

ISSN:0749-503X    

DOI:10.1002/yea.320090810

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

摘要:

AbstractThe nucleotide sequence of a fragment of 4337 base pairs ofSaccharomyces cerevisiaechromosome II has been determined. The sequence contains three open reading frames, one of them being incomplete. Deletion analysis showed that YBR12.31 is essential for yeast growth, while deletion mutants of YBR12.32 and YBR12.33 are viable. YBR12.33 is identical toSMY2, isolated as a suppressor of amyo2mutant (Lillie, S.H. and Brown, S.S., unpublished, EMBL M90654).

ISSN:0749-503X    

DOI:10.1002/yea.320090811

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY