摘要:

AbstractA new secreted yeast glycoprotein with an Mrof about 400 kDa (gp400) has been found. The glycoprotein is an O‐mannosylated oligomer, whose synthesis and export into culture medium are stimulated by heat shock. Intracellular transport of gp400 is carried out by membrane vesicles distinct form the known constitutive scretory vesicles. Immunological analysis revealed gp400 only inSaccharomycesspecie

ISSN:0749-503X    

DOI:10.1002/yea.320080302

出版商:John Wiley&Sons, Ltd.    

年代:1992

数据来源: WILEY

摘要:

AbstractThe architectural features of 73 introns found in 36 genes of the fission yeastSchizosaccharomyces pombehave been compiled and tabulated. The intron features ofSaccharomyces cerevisiaand other eukaryotes. The results thatS. pombedisplays quite different architectural features than the budding yeastS. cerevisiae. However, particularly in the 3′ region,S. pombeintrons also appear to differ from mammalian intron

ISSN:0749-503X    

DOI:10.1002/yea.320080303

出版商:John Wiley&Sons, Ltd.    

年代:1992

数据来源: WILEY

摘要:

AbstractThecif1mutation ofSaccharomyces cerevisia(Navonet al., Biochemistry18, 4487–4499, 1979) causes inability to grow on glucose and absence of catabolite inactivation. We have cloned theCIF1gene by complementation of funcion and licated it in a 2·75 kbSphI‐BstEII fragment situated at ca. 18 kb centomere distal ofLYS2and ca. 80 kb centromere proximal ofTYRIon chromosome II. Southern analysis demostrated thatCIF1is present in a single copy in the yeast genome. Northern analysis revealed that the corresponding mRNA of 1·8 kb is more abundant in cells grown on galactose than in those grown on glucose. A protein of ca. 54 kDa was predicted from the open reading frame in the sequenced fragment. In strains carrying thecif1mutation the intracellular concentration of ATP decreased immediately after addition of glucose while the intracellular concentration of cAMP did not increse. cAMP concentration increases in response to galactose or 2,4‐dinitrophenol. Disruption ofBCY1or overexpression ofCDC25in acif1/, background did not restore growth on glucose, suggesting that the absence of cAMP signal is not primary cause of lack of growth on glucose. Complementation tests showed thatcif1is not allelic tofdp1although the two genes seem to be functionally

ISSN:0749-503X    

DOI:10.1002/yea.320080304

出版商:John Wiley&Sons, Ltd.    

年代:1992

数据来源: WILEY

摘要:

AbstractThe expression of the glyoxylate cycle enzymes is required for growth of the yeastYarrowia lipolyticaon acetate or fatty acids as sole carbon source. Acetyl‐coenzyme A, which is produced by acetyl‐coenzyme A synthetase (ACS) from acetate, is needed for induction of this expression. Acetate‐non‐utilizing mutants of this yeast were investigated in order to identify mutants which express no or strongly reduced activity of this enzyme. Mutations in geneICL2exhibited the strongest effects on the activity. Inicl2mutants, lack of ACS activity resulted in a non‐induced glyoxylate cycle on acetate; however, induction on fatty acids was not affected. GeneICL2was identified as the sstructural gene encoding the monomer of ACS. It is shown that a high level of ACS activity is necessary for full expression of the glyoxylate cycle enzymes. Mutations in geneICL1, which encodes isocitrate lyase, resulted in overproduction of ACS without any growth on acetate. A new gene (GPR1= glyoxylate pathway rergulation) was detected in whichtrans‐dominant mutations inhibit expression of ACS and the glyoxylate cycle on acetate as ca

ISSN:0749-503X    

DOI:10.1002/yea.320080305

出版商:John Wiley&Sons, Ltd.    

年代:1992

数据来源: WILEY

摘要:

AbstractWe have determined the nucleotide sequence of a segment from chromosome III foSaccharomyces cerevisiaeextending over 7·9 kb between thePGK1andCRY1loci.The fragment contains seven open reading frames, YCR241, YCR242, YCR243, YCR244, YCR245, YCR246 and YCR247, of more than 70 codons. The study of the effects of a global disruption of YCR242, YCR243, YCR244, YCR245 and YCR247 shows that they are not essential for growth division

ISSN:0749-503X    

DOI:10.1002/yea.320080306

出版商:John Wiley&Sons, Ltd.    

年代:1992

数据来源: WILEY

摘要:

AbstractA 10,095 base DNA fragment from the right arm of chormosome III ofSaccharomyces cerevisiaehas been sequenced and analysed. It encompasses the silent mating‐type locusHMR. BothHR Ma1 andHM Ra2 genes, as well as their flanking regulatory regions, have been identified. Three new open reading frames longer than 80 amino acid residues were found in this fragment. One of them (YCR137) shows features compatible with a membranous localization and a tansporter function. The other two do not show a similarity with any known gene. A new gene coding for tRNAthra1 (ACU) has been identified. It is located in a region coding for several delta sequence

ISSN:0749-503X    

DOI:10.1002/yea.320080307

出版商:John Wiley&Sons, Ltd.    

年代:1992

数据来源: WILEY

ISSN:0749-503X    

DOI:10.1002/yea.320080308

出版商:John Wiley&Sons, Ltd.    

年代:1992

数据来源: WILEY

摘要:

AbstractThe nucleotide sequence of 6472 base pairs of an 8·2 kb segment ofSaccharomyces cerevisiaechromosome XI has been determined. The sequence contains a cluster of four long open reading frame (ORF) designatedYKL2, YKL3, YKL4andTGL1in the same orientation, flanked at the 5′‐end by a divergent incomplete ORF (YKL1). Transcription and Southern analyssis of the four complete ORFs showed that all are expressed and are present in single copy on the haploid genome. The average codon adaption index of the coding regions is approximately 0·2, suggesting that these genes are lowly expressed. The upstream regions of all four genes as well as theYKL1ORF contain putative promoter elements previously found to be characteristic of nuclear genes encoding mitochondrial proteins. Significant sequence similarities were found between theYKL3protein andEscherichia coliribosomal protein S2 as well as between theTGL1protein and triglyceride lipases from rat salivary gland and human gastric tissue. The 3′‐end of the 6472 bp nucleotide sequence overlaps with the upstream region of the previously identifiedCTK1gene, encoding the largest subunit of CTD kinase (Lee, J. M. and Greenleaf, A. L., 1991,Gene Expression2, 149–167), thereby increasing the number of genes on the 8·2 kb fragment to at least five. The transcripts of these genes represent approximately 83% of the DNA fragment, making it one of the most highly transcribed regions of the yeast chromosome anal

ISSN:0749-503X    

DOI:10.1002/yea.320080309

出版商:John Wiley&Sons, Ltd.    

年代:1992

数据来源: WILEY

ISSN:0749-503X    

DOI:10.1002/yea.320080310

出版商:John Wiley&Sons, Ltd.    

年代:1992

数据来源: WILEY

ISSN:0749-503X    

DOI:10.1002/yea.320080311

出版商:John Wiley&Sons, Ltd.    

年代:1992

数据来源: WILEY