ISSN:0749-503X    

DOI:10.1002/yea.320080102

出版商:John Wiley&Sons, Ltd.    

年代:1992

数据来源: WILEY

摘要:

AbstractYeast floccultion results from surface expression of specific proteins (lectins). Two flocculation phenotypes were suggested by physiological and biochemical tests, whereas genetic data suggested a larger number of mechanisms of flocculation. After reviewing the biochemistry, physiology and genetics of flocculation, a new hypothesis combining the data available from these different sources, is proposed.Flocculation results when lectins present on flocculent cell bind sugar residues of neighbouring cell walls. These sugar receptors are intrinsic to the mannan comprizing cell walls ofSaccharomyces cerevisiae. Two lectin phenotypes were revealed by sugar inhibition studies. The gluco‐ and mannospecific NewFlo phenotype is not, as yet, found in genetically defined strains. Mannospecific flocculation (Flo 1 phenotype) is found in strains containing the genesFLO1, FLO5andFLO8. This phenotype is also found following mutation of theTUP1orCYC8loci, in previously non‐flocculent strains. It is therefore proposed that the structure gene for mannospecific flocculation is common or possibly unbiquitous in non‐flocculent strains and in consequence,FLO1, FLO5andFLO8are probably regulatory genes, exerting positive control over the structure gene.Flocculation expression requires lectin secretion to the cell surface. Many of the observed ‘suppressions’ of flocculation may be due to mutations of the secretory process, involved in transporting structural proteins to the cell wall.The possible involvement of killer L double‐stranded RNA with flocculation is suggested, given the lectin properties of viral coat proteins nad an association between L double‐stranded RNA and the Fl

ISSN:0749-503X    

DOI:10.1002/yea.320080103

出版商:John Wiley&Sons, Ltd.    

年代:1992

数据来源: WILEY

摘要:

AbstractSimple methods, based on the technique of flow cytometry, have been developed for the phenotypic characterization of yeast autolytic mutants and for the analysis of the formation and regeneration of the yeast protoplasts. The expression of lytic mutations determined uptake of the fluorescent dye propidium iodide, which could be carefully monitored by flow cytometry. Mixed populations of lysed and viable cells were precisely quantified and sorted, and the technique was also applied to demonstrate protection from lysis of mutant cells with cell wall defects, in the presence of osmotic stabilizers. Protoplast formation and regeneration was monitored by analysing relative cell size; this was facilitated by the preparation of homogeneous protoplast preparations. The technique of flow cytometry proved superior to other conventional methods for these types of study.

ISSN:0749-503X    

DOI:10.1002/yea.320080104

出版商:John Wiley&Sons, Ltd.    

年代:1992

数据来源: WILEY

摘要:

AbstractIn this report the effects of phosphoglycerate kinase (PGK) overproduction on the physiology and plasmid stability in baker's yeastSaccharomyciae cerevisiaecontaining thePGK1gene on an episomal plasmid are described. This examination reveals that there is a preferred intracellular level for this enzyme, amounting to 10–15% of the total soluble protein. Strains containing the plasmid and the host strain were grown in non‐selective batch cultures and continuous culture, under different growth conditons. Plasmid‐containing yeast strains stabilize the copy number of the episomal plasmid at a level at which the PGK concentration is about 12%. This stabilization is due to an equilibrium between normal plasmid loss and selective pressure because of advantages resulting from the increased amount of PGK under glucose‐limited conditions. During respiro‐fermentative growth, PGK‐overproducing cells showed an increased respiration rate and decreased fermentative activity, compared to the host strain.ThePGK1gene can be applied as a direct positive selection marker to obtain a high episomal plasmid stability during growth on glucose. The results are consistent with previously reported data on the physiology and gene stability of PGK‐overproducing yeast cells that contain multiple copies of thePGK1gene integrated in

ISSN:0749-503X    

DOI:10.1002/yea.320080105

出版商:John Wiley&Sons, Ltd.    

年代:1992

数据来源: WILEY

摘要:

AbstractA platelet‐derived endothelial cell growth factor cDNA has been cloned, sequenced and expressed using theSaccharomyces cerevesiae PRBIpromoter. Soluble recombinant platelet‐derived endothelial cell growth factor constituted 0·5–1·0% of total soluble protein. Yeast soluble protein extracts containing recombinant platelet‐derived endothelial cell growth factor stimulate the growth of calf palmonary artery endothelial cell

ISSN:0749-503X    

DOI:10.1002/yea.320080106

出版商:John Wiley&Sons, Ltd.    

年代:1992

数据来源: WILEY

摘要:

AbstractThe complete nucleotide sequence of the D10H fragment (10850 bp) was determined. The D10H fragment is located on the right arm of chromosome III near the centromere and contains theSUF2gene. Six open reading frames (ORFs) larger than 300 bp were found. One of them is theCIT2gene encoding the cytoplasmic citrate synthase. The others are new putative genes and show no significant similarly with any known gene. In addition two tRNA genes (Asn and Pro) and a solo delta element were identified. Two ORFs were disrupted; no peculier phenotype was observed.

ISSN:0749-503X    

DOI:10.1002/yea.320080107

出版商:John Wiley&Sons, Ltd.    

年代:1992

数据来源: WILEY

摘要:

AbstractWe have sequenced a gene on chromosome III ofSaccharomyces cerevisiaewhich codes for a putative serine/threonine protein kinase of 726 amino acids (calculated molecular weight 82 kDa). We have called this geneKIN82. The amino acid sequence ofKIN82is most similar to the cyclic nucleotide‐dependent protein kinase subfamily and the protein kinase C subfamily. Gene disruption ofKIN82did not produce any phenotype when tested under a variety of conditons. Reduced stringency hybridizations revealed the presence of another genomic sequence with high homology to the carboxy‐terminal catalytic domain ofKI

ISSN:0749-503X    

DOI:10.1002/yea.320080108

出版商:John Wiley&Sons, Ltd.    

年代:1992

数据来源: WILEY

ISSN:0749-503X    

DOI:10.1002/yea.320080101

出版商:John Wiley&Sons, Ltd.    

年代:1992

数据来源: WILEY