ISSN:0749-503X    

DOI:10.1002/yea.320111502

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

摘要:

AbstractThemae1gene of the yeastSchizosaccharomyces pombewas identified on the basis of its ability to complement a mutant defective in the transport of malic acid. Analysis of the DNA sequence revealed an open reading frame of 1314 base pairs, encoding a polypeptide of 438 amino acids with a predicted molecular weight of 49 kDa. A hydropathy profile of the predicted amino acid sequence revealed a protein with ten membrane‐spanning or associated domains and hydrophilic N‐ and C‐ termini. The predicted secondary structure of the protein is similar to models proposed for other integral mambrane proteins from both prokaryotes and eukaryotes. TheS. pombe mae1gene encodes a single mRNA of 1·5 kb. Themae1gene is expressed constitutively and is not subject to catabolite repression as was previously reported for the malate permease systems ofCandida utilisandHansenula anomala. Themae1gene was mapped 2842 bp 5′ to theMFm1gene on chromosome I. Transport assays revealed that themae1gene encodes a permease involved in the uptake of L‐malate, succinate and malonic acid. The sequence of theS. pombe mae1gene is available in GenBank under Accession Num

ISSN:0749-503X    

DOI:10.1002/yea.320111503

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

摘要:

AbstractIn the presence of glycerol or ethanol, SCPP (a strain ofSaccharomyces cerevisiaethat expresses pectinolytic activity) is capable of utilizing galacturonic acid or pectins for growth purposes. We now establish a relationship between the pectinolytic power of various strains ofS. cerevisiaeand their ability to grow on a pectin/glycerol‐based medium. This property is further exploited for the detection of polygalacturonase‐producing strains ofS. cerevis

ISSN:0749-503X    

DOI:10.1002/yea.320111504

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

摘要:

AbstractCandida glabratastrains and a stable plasmid were developed that were suitable for analysis of copper‐inducible expression from promoters of the three metallothionein (MT) genes. The two homologous MTII genes,MTIIaandMTIIb, encode the same polypeptide but are differentially induced by copper salts.MTIIbis more highly inducible thanMTIIaand cells harboring a singleMTIIbexhibit a greater resistance to copper salts compared to cells harboring a singleMTIIa. The differential copper inducibility was mapped to sequences between − 503 and − 292 upstream of the MT coding sequences. Expression ofMTIis highly Cu‐regulated, but this MT gene confers much less resistance than MTI

ISSN:0749-503X    

DOI:10.1002/yea.320111505

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

摘要:

AbstractWe have cloned and sequenced theGDS1gene located on the right arm of chromosome XV ofSaccharomyces cerevisiae. The gene codes for a 522 amino acid serine‐rich protein with no obvious homology to proteins in the database.GDS1gene was isolated as the multicopy suppressor of the glycerol‐deficient phenotype caused by thenam9‐1mutation in the yeast nuclear gene encoding the mitochondrial ribosomal protein homologous to S4 proteins from various organisms. Disruption‐deletion of theGDS1open reading frame leads to a partial impairment of growth on medium containing glycerol as the carbon source, indicating mitochondrial function of the gene product. The sequence has been deposited in the GenBank data library under Accession Number

ISSN:0749-503X    

DOI:10.1002/yea.320111506

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

摘要:

AbstractThe complete sequence of a 11,132 bp segment located on the left arm of chromosome VII ofSaccharomyces cerevisiaehas been determined and analysed. Eight open reading frames (ORFs) of at least 100 amino acids were identified. Five show similarities to known amino acid sequences. Another ORF encoding the chromosome segregation protein CSE1 is not entirely located in our sequenced fragment and is incomplete at its C‐terminus. The two remaining ORFs do not display similarities to known sequences. The sequence has been deposited in the EMBL Data Library under Accession Number Z4914

ISSN:0749-503X    

DOI:10.1002/yea.320111507

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

摘要:

AbstractThe nucleotide sequence of lambda phage clone 4121, which contains the 18·8 kb fragment ofSaccharomyces cerevisiaechromosome VI left arm, was determined. This sequence had seven open reading frames (ORFs), four of which were identical to known genes (ACT1, YPT1, TUB2andRPO41). Another three ORFs (4121orfR003, 4121orfR004 and 4121orfRN001) were highly homologous toFET3multi‐copper oxidase, glucose transport protein, and hypothetical protein of YIL106w on chromosome IX, respectively. 4121orfRN01 is suggested to contain an intron. The sequence has been submitted to DDBJ/EMBL/GenBank data library under Accession Number D445

ISSN:0749-503X    

DOI:10.1002/yea.320111508

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

摘要:

AbstractWe have cloned and sequenced thehcsgene, which is thought to encode a 3‐hydroxy‐3‐methylglutaryl coenzyme A (HMG‐CoA) synthase consisting of 447 amino acids, from the fission yeastSchizosaccharomyces pombe. The predicted amino acid sequence of thehcsproduct ofS. pombehas homology with the HMG‐CoA synthase of rat (47·8%), chicken (49·2%), hamster (47·1%) and human cells (46·9%). One of thehcsgenes was replaced with a marker gene in the diploid cell. No viablehcs‐disrupted haploid was isolated after tetrad dissection, suggesting that thehcsgene is essential for growth. However thehcs‐defective mutant could be grown on a medium containing 5 mg/ml mevalonate. These results strongly support that thehcsgene encodes HMG‐CoA synthase andS. pombecontains a single copy of thehcsgene. The sequence of thehcsgene has been entered into the public data libraries under Acce

ISSN:0749-503X    

DOI:10.1002/yea.320111509

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

ISSN:0749-503X    

DOI:10.1002/yea.320111510

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

ISSN:0749-503X    

DOI:10.1002/yea.320111501

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY