ISSN:0749-503X    

DOI:10.1002/yea.320110602

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

摘要:

AbstractThe regulatory step Start in the cell cycle of the budding yeastSaccharomyces cerevisiaeis inhibited by nalidixic acid (Nal). To study this inhibition, mutations were identified that alter the sensitivity of yeast cells to Nal. Nal‐sensitive mutations were sought because the inhibitory effects of Nal on wild‐type cells are only transient, and wild‐type cells naturally become refractory to Nal. Three complementation groups of Nal‐sensitive mutations were found. Mutations in the first complementation group were shown to reside in theARO7gene, encoding chorismate mutase; tyrosine and phenylalanine synthesis was inhibited by Nal in thesearo7mutants, whereas wild‐type chorismate mutase was unaffected. Thesearo7alleles demonstrate ‘recruitment’, by mutation, of an innately indifferent protein to an inhibitor‐sensitive form. The Nal‐sensitivearo7mutant cells were used to show that the resumption of Nal‐inhibited nuclear activity and cell proliferation takes place while cytoplasmic Nal persists at concentrations inhibitory for the mutant chorismate mutase. Mutations in the second complementation group,nss2(Nal‐supersensitive), increased intracellular Nal concentrations, and may simply alter the permeability of cells to Nal. The third complementation group was found to be theERG6gene, previously suggested to encode the ergosterol biosynthetic enzyme sterol methyltransferase. Mutation or deletion of theERG6gene had little effect on the inhibition of Start by Nal, but prevented recovery from this inhibition. Mutation ofERG3, encoding another ergosterol biosynthetic enzyme, also caused Nal sensitivity, suggesting that plasma membrane sterol composition, and plasma membrane function, mediates recovery from Nal‐media

ISSN:0749-503X    

DOI:10.1002/yea.320110603

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

摘要:

AbstractIn experiments with yeast cells it was shown that the synergistic effect of a simultaneous action of ionizing radiation and hyperthermia was a function of dose rate. It was demonstrated that, with increasing dose rate to obtain maximum synergistic effect, the temperature at which radiation is delivered should be elevated. Possible explanation of this effect is discussed.

ISSN:0749-503X    

DOI:10.1002/yea.320110604

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

摘要:

AbstractThe goal of the present work is the construction of software (EXPLORA) which automatically detects Open Reading Frames (ORF), intron‐containing or not, in the nuclear sequences of the yeastSaccharomyces cerevisiae. In order to detect introns within (or preceding) ORFs, one must define precise rules of how an intron is identified. These rules are described here. The software is able to detect correctly an intron‐associated ORF in at least 88% of cases. We tested all yeast nuclear entries of the EMBL database with the software, and found, in addition to the known intron‐associated ORFs, some others which may include an intron. Finally the software was applied to the DNA sequences of chromosomes III and XI, in which it detected eight new intron‐associat

ISSN:0749-503X    

DOI:10.1002/yea.320110605

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

摘要:

AbstractThe DNA sequence of a 15·4 kb region covering the left arm of chromosome XIV fromSaccharomyces cerevisiaewas determined. This region contains eight open reading frames (ORFs) which code for proteins of more than 100 amino acids. Three ORFs correspond to theRPD3, PAS8andKRE1loci, described previously. Three ORFs show limited homology with known proteins: NO330 with the recessive suppressor of secretory defectSAC1, NO325 with YCR094W identified during chromosome III sequencing; whereas NO315 presents a motif conserved in the dnaJ family. Two ORFs (NO320 and NO325) show no homology to known proteins within the databases screened, but NO320 corresponds to a serine‐threonine‐rich protein. The sequence has been entered in the EMBL data library under Accession Number Z4

ISSN:0749-503X    

DOI:10.1002/yea.320110606

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

摘要:

AbstractWe present the upstream sequences ofHSP82andHSC82, two closely related, but differentially regulated, heat‐shock genes ofSaccharomyces cerevisiae. Several dozen potential regulatory elements are identified within each upstream region; interestingly, only a few are conserved between the two genes. These include a consensus heat‐shock element, an upstream repressor element, and a consensus TATA element. A search for motifs known actively to position nucleosomesin vitrorevealed that such sequences are three‐ to seven‐fold enriched within each promoter; a comparable enrichment is seen near the 3′ end of each transcription unit. Located ∼ 1100 bp upstream ofHSC82is an open reading frame (ORF) of 255 amino acids; ∼ 800 bp upstream ofHSP82is an ORF of 132 amino acids. The latter ORF contains several conserved ankyrin motifs and appears to be expressed under normal growth conditions. Finally, we show by clamped homogeneous electric field gel electrophoresis that the two genetic loci map to different chromosomes:HSP82to chromosome XVI andHSC82to chromosome XIII. The sequences have been deposited in the GenBank database under Accession Numbers U203

ISSN:0749-503X    

DOI:10.1002/yea.320110607

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

摘要:

AbstractThe nucleotide sequence of yeast chromosome III encompassing the previously described open reading frames (ORFs) YCR80w, YCR77c and YCR78c (Oliveret al., 1992) has been updated. In the corrected sequence, these ORFs are replaced by two new ORFs, YCR80w (453 bp) and YCR77c (2391 bp). In addition, the orientation of Ycr79c is reversed to give ORF Ycr79w, which has an unaltered nt sequence. The predicted translation products do not exhibit significant homology to known proteins. ORF Ycr77p encodes an 88 kDa, cytosolic protein. A fraction of the protein is associated with small membranous structures in a salt‐sensitive fashion. Initial characterization revealed that the protein is not essential for yeast viability, growth on non‐fermentable carbon sources, mating and sporulation. The chromosome III DNA sequence that was used for the analysis has the Accession Number X59720 in the GenBank/EMBL datab

ISSN:0749-503X    

DOI:10.1002/yea.320110608

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

摘要:

AbstractWe report the sequence of a 9037 bp fragment from the right arm ofSaccharomyces cerevisiaechromosome VII. Analysis of the sequence revealed four complete open reading frames (ORFs), namely G7572, G7576, G7579 and G7584. The first three corresponded, respectively, to the previously cloned genes:HIP1, coding for a high‐affinity histidine‐specific permease,TDH1, one of the known genes coding for glyceraldehyde‐3‐phosphate dehydrogenase andODPX, which encodes a precursor of protein X, a component of the pyruvate dehydrogenase complex. The ORF G7584 showed 35·8% identity with a hypothetical protein ofCaenorhabditis eleganschromosome 3. The reported sequence has been deposited in the EMBL data library under Accession Numbe

ISSN:0749-503X    

DOI:10.1002/yea.320110609

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

ISSN:0749-503X    

DOI:10.1002/yea.320110610

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

ISSN:0749-503X    

DOI:10.1002/yea.320110601

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY