摘要:

AbstractThe determination of the enantiomeric impurity, i.e., the percentage of (+) N−0437 (= N−0924) in several batches of (−−) N‐0437 (= N‐0923) by chiral HPLC is described. Enantiomeric impurities were calculated based on the peak areas of the two baseline separated enantiomers in the chromatogram. The enantiomeric impurities found in different batches ranged from 0.02% to 0.11%. Calibration curves of the two isomers of N‐0437 (Fig. 1,) were made twice to study the reproducibility and linearity of the method. The absorbance ratio, N‐0923/N‐0924, was found to be 1.02 with a relative standard deviation (RSD) of 9% over the whole concentration range used for the c

ISSN:0899-0042    

DOI:10.1002/chir.530040113

出版商:Alan R. Liss, Inc.    

年代:1992

数据来源: WILEY

摘要:

AbstractRacemic 4',6‐dichloroflavan (BW683C), a highly effective inhibitor of rhinovirus serotype 1B in vitro, was resolved by high‐performance liquid chromatography on a chiral stationary phase. The enantiomers were separately collected and circular dichroism curves were obtained, in order to determine the absolute configuration of the two enantiomers. The activity of the isomers was studied on human rhinovirus serotype 1B multiplication in HeLa cell cultures, by means of the plaque reduction assay. Both enantiomers were potent inhibitors of virus replication; by comparing the IC50values, the S form was 3.5 times more effective than the R f

ISSN:0899-0042    

DOI:10.1002/chir.530040114

出版商:Alan R. Liss, Inc.    

年代:1992

数据来源: WILEY

ISSN:0899-0042    

DOI:10.1002/chir.530040115

出版商:Alan R. Liss, Inc.    

年代:1992

数据来源: WILEY

ISSN:0899-0042    

DOI:10.1002/chir.530040116

出版商:Alan R. Liss, Inc.    

年代:1992

数据来源: WILEY

ISSN:0899-0042    

DOI:10.1002/chir.530040102

出版商:Alan R. Liss, Inc.    

年代:1992

数据来源: WILEY