ISSN:0147-8885    

DOI:10.1179/his.1996.19.4.293

出版商:Taylor&Francis    

年代:1996

数据来源: Taylor

摘要:

AbstractAcrylic resin mixtures are now widely used as embedding media for the preparation of tissue sections. Most of these mixtures are based on 2-hydroxyethyl methacrylate (glycol methacrylate, GMA). Resin embedding preserves tissue components far better than paraffin, celloidin or frozen sections. The present review describes the basic principles and trouble shooting, in particular: the chemical and physical properties of GMA, and components used for GMA mixtures; fixation of tissues for resin embedding; methods for dehydration; microtomy; stretching on water and mounting in relation to the final dimensions of GMA sections; staining of GMA 3embedded tissue sections; and the use of GMA resins in immunohistochemistry. In addition, standard, step by step procedures for embedding tissues in GMA is included. (The J Histotechnol19:297–311, 1996)

ISSN:0147-8885    

DOI:10.1179/his.1996.19.4.297

出版商:Taylor&Francis    

年代:1996

数据来源: Taylor

摘要:

AbstractOsteoblasts undergo changes both in shape (progressive flattening) and function (progressive decline in matrix synthesis) during their life span. Nuclear volume, apart from changing systematically during their cell cycle, is believed to be a general index of cell vigor. Accordingly, we measured the nuclear profile area of osteoblasts classified as pre (Group I), cuboidal (Group II), intermediate (Group III), and terminal (Group IV) in iliac cancellous bone in 6 premenopausal normals, mean age in years (SD) 40.2 (4.8), 5 postmenopausal normals, age 65.8 (2.4), 15 postmenopausal osteoporotics, age 65.3 (4.9), 5 patients with fluorideinduced osteomalacia, age 69.2 (5.0), and two patients with endemic fluorosis, age 70.5 (5.5). A total number of 4,085 osteoblast nuclei were examined and measured. In each group there was a systematic decline in nuclear profile area through stages I-IV, corresponding to an average decline in nuclear volume of greater than 90%. The major difference among groups was that in type II (cuboidal) osteoblasts, nuclear profile area was significantly greater in premenopausal normals than in the other four groups, which did not differ significantly. The effect seems to be related to age, rather than to disease or treatment. The functional significance of this age-related morphologic change remains to be determined, but it may be related to the decline in apposition rate with age. (The J Histotechnol19:313–315, 1996)

ISSN:0147-8885    

DOI:10.1179/his.1996.19.4.313

出版商:Taylor&Francis    

年代:1996

数据来源: Taylor

摘要:

AbstractBone marrow smears fixed in formalin-95% ethyl alcohol- glacial acetic acid (FAA), stained with a paper dye known as basic black 05L, exposed briefly to 1 N hydrochloric acid, and mounted with cyanoacrylate adhesive displayed a bright and distinctive violaceous cytoplasmic color in megakaryocytes. Except for mast cell granules that stained violet, all other hematopoietic cells in normal and abnormal marrow specimens stained yellow to yellow orange. Normal and abnormal megakaryocytes, such as unusually large megakaryocytes in essential thrombocythemia and small megakaryocytes in myelodysplastic disorders, displayed this acid resistant cytoplasmic metachromasia. The stain is inexpensive, easy to use, and may prove usefu1 for identification of atypical and small megakaryocytes in myeloproliferative and myelodysplastic disorders. (The J Histotechnol19:317–320, 1996)

ISSN:0147-8885    

DOI:10.1179/his.1996.19.4.317

出版商:Taylor&Francis    

年代:1996

数据来源: Taylor

摘要:

AbstractA variation of the Gomori trichrome stain has been developed to emphasize the morphology of gross specimens. The stain results in good contrast among various kinds of tissue. The tissue distortion associated with paraffin embedding and microtome sectioning is avoided. The stain has been used on full-thickness sections of human abdominal wall to produce differences in coloration of fat, muscle, and connective tissue for subsequent scanning and analysis by computer. (The J Histotechnol19:321–323, 1996)

ISSN:0147-8885    

DOI:10.1179/his.1996.19.4.321

出版商:Taylor&Francis    

年代:1996

数据来源: Taylor

摘要:

AbstractThe authors describe a modification of Movat pentachrome stain for the demonstration of elastin, sulfated macromolecules (proteoglycans, glycosaminoglycans and secreted mucins), collagens, myofibrils, and erythrocytes. The key modification is the use of a modified Verhoeff elastic tissue stain, which is more tolerant of minor variations in technique and does not require differentiation to a subjective endpoint. The resulting stained sections have a clear background and crisply defined cell and matrix components including both large and small elastic fibers. This stain is particularly useful for evaluating the matrix components of lung and vascular tissue in normal and diseased states. (The J Histotechnol19:325–327, 1996)

ISSN:0147-8885    

DOI:10.1179/his.1996.19.4.325

出版商:Taylor&Francis    

年代:1996

数据来源: Taylor

摘要:

AbstractA modified Gomori technique for the assessment of frozen skeletal muscle and all paraffin embedded sections is presented. The modified Gomori solution consists of 0.015% fast green FCF, 0.12% chromotrope 2R, 0.65% phosphotungstic acid and 1.0% acetic acid in 100 ml distilled water. Frozen skeletal muscle staining of nuclei is done with celestine blue followed by hematoxylin; paraffin sections are stained with a modified Verhoeff hematoxylin. This method allows demonstration of nemaline rods, cytoplasmic inclusion bodies and other abnormalities in frozen skeletal muscle and takes about 12 minutes to perform. In paraffin sections the staining results are similar to the Masson trichrome method with less manipulation, cleaner background and staining time is about 17 minutes. (The J Histotechnol19:329–333, 1996)

ISSN:0147-8885    

DOI:10.1179/his.1996.19.4.329

出版商:Taylor&Francis    

年代:1996

数据来源: Taylor

摘要:

AbstractImmunohistochemical markers were used to demonstrate subtle epithelial differentiation within the mononuclear inflammatory cell infiltrate of lymphoepithelioma-like carcinoma of the uterine cervix. (The J Histotechnol19:335–337, 1996)

ISSN:0147-8885    

DOI:10.1179/his.1996.19.4.335

出版商:Taylor&Francis    

年代:1996

数据来源: Taylor

摘要:

AbstractA consistent1procedure for the Warthin-Starry stain technique for melanin and spirochetes is described. Close attention to solution preparation, purity, and pH, freshness of chemicals used, and rigorous adherence to the protocol is essential. The procedures and recommendations described ensure consistent success when using this often difficult stain technique. (The J Histotechnol19:339–340, 1996)

ISSN:0147-8885    

DOI:10.1179/his.1996.19.4.339

出版商:Taylor&Francis    

年代:1996

数据来源: Taylor

摘要:

AbstractAn alternative to picric acid as a mordant in the staining of cytoplasm, keratin, muscle fibers, and intercellular fibers in the Masson trichrome stain is a citrate acid-sodium citrate buffer used as the microwave pretreatment of the tissue mounted on a slide. The Masson trichrome stain, a combination of Biebrich scarlet-acid fuchsin and aniline blue dye can be done within 1 hr and avoids the hazards associated with picric acid, a highly explosive compound in the dry state. (The J Histotechnol19:341–342, 1996)

ISSN:0147-8885    

DOI:10.1179/his.1996.19.4.341

出版商:Taylor&Francis    

年代:1996

数据来源: Taylor