ISSN:0147-8885    

DOI:10.1179/his.1993.16.4.311

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

AbstractNonisotopic in situ hybridization (ISH) for polyadenylated messenger RNA (mRNA) sequences was performed on a variety of tissues fixed in 3 commonly used fixatives, 100% ethanol, 10% neutral buffered formalin (NBF), and Bouin solution. The method utilized a synthetic 20 base biotin-labeled poly T oligonucleotide probe in combination with capillary action technology and was performed in under 2 hr. Poly A sequences were detected in tissues fixed with all 3 fixatives; however, optimal protocols varied between aldehyde fixed and alcohol fixed tissues. For alcohol fixed tissues, poly A detection was achieved with a protocol that excluded the use of prehybridization protease and acid denaturation. These parameters were necessary in aldehyde tissues for optimal detection of poly A sequences. Poly A sequences were seen in tissues fixed in ethanol or NBF from 2–216 hr. Strong signal was identified in tissues fixed inBouin for less than 8 hr after which signal was decreased or not present, regardless of the method used. ISH with the poly T oligonucleotide may be a rapid way to determine the suitability of a routinely processed block for in situ mRNA hybridization.(The J Histotechnol16:315, 1993)

ISSN:0147-8885    

DOI:10.1179/his.1993.16.4.315

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

AbstractThe effects of aldehyde fixation (with and without osmium treatment) on the localization of prolactin immunoreactivity was studied with immunofluorescence, immunoperoxidase, and immunogold methods. Plastic and frozen sections (adjacent semithin and ultrathin) were compared. Pituitary glands from postlactating female rat were fixed either by paraformaldehyde-glutaraldehyde (PG) or paraformaldehyde-lysineperiodate (PLP) fixative and then treated with or without osmium tetroxide.At the light microscope level, prolactin immunoreactivity was heavily located in the aldehyde fixed, plastic embedded tissues. Immunoreactive product was slightly heavier in the PG fixed, plastic embedded sections than in the PLP fixed, plastic embedded ones. In PG-osmium fixed, plastic embedded tissues, prolactin immunoreactivity was reduced, and nuclei of adenohypophysial cells and basement membranes of the blood vessels were also lightly immunolabeled. In PLPosmium fixed, plastic embedded tissues, prolactin immunoreactivity was slightly reduced, and the immunostaining pattern remained similar to that in PLP fixed, plastic embedded or PLP fixed,frozen sectioned tissues.At the electron microscope level, immunoreactive product was found on the surface of the secretory granules in the PG or PLP fixed, plastic embedded tissues. However, in the PLP fixed frozen sectioned tissues, prolactin immunoreactivity was located on the surface of the secretory granules and the Golgi complex. (The J Histotechnol16:323, 1993)

ISSN:0147-8885    

DOI:10.1179/his.1993.16.4.323

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

AbstractAlzheimer neurofibrillary changes and brainβ-amyloid can be stained selectively by silver impregnation on tissue sections and (in the case of neurofibrillary proteins) on sodium dodecyl sulfate-polyacrylamide gels. The stain can be applied to frozen sections of tissue from 10–150μm thick, to similarly thick polyethylene glycol sections, or to 5–15μm thick paraffin sections. The technique can also be applied to routinely fixed autopsy material. The technique takes advantage of the physical development of nucleation sites, thereby permitting tight control of the entire procedure. It is less expensive than immunocytochemical techniques, and facilitates processing of large numbers of sections through entire hemispheres of the human brain. (The J Histotechnol16:335, 1993)

ISSN:0147-8885    

DOI:10.1179/his.1993.16.4.335

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

AbstractThe histological effects of freezing and thawing unfixed tissue before experimental diffusion studies across the mucosal barrier were investigated in the face of claims that such treatment was of little significance. Following fixation, tissue sections were stained by periodic acid-Schiff (PAS), alcian blue (pH 1.0&2.5)-PAS, Masson and Mallory trichromes, acid-picro-Mallory phosphotungstic acid-hematoxylin, Martius-scarlet-blue, luxol-fast-blue, and Sudan black B. Trichrome type staining revealed a previously unrecorded artifact, particularly evident in quenched tissues. Three distinct epithelial cell types could be identified on the basis of differential dye uptake. This finding was not evident in PAS, alcian blue, or Sudan black B stained sections. We postulate that distortion of the cellular matrix occurs as a result of the freeze, thaw, diffusion, and fix sequence and that these cells illustrate the well known phenomenon of tissue density/dye molecular size differential staining by acid dyes. (The J Histotechnol16:343, 1993)

ISSN:0147-8885    

DOI:10.1179/his.1993.16.4.343

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

AbstractThree connective tissue methods are presented: modifications of Masson trichrome, Movat pentachrome and a fibrin method. A modified Verhoeff hematoxylin preheated and applied in the 60°C paraffin oven was used for all methods. The Movat pentachrome modification additionally included staining with alcian blue before application of Verhoeff hematoxylin, and the fibrin method was stained with lissamine fast yellow before application of the working red stain. All sections were stained with a working dilution of Biebrich scarlet and acid fuchsin, rinsed, and differentiated with phosphotungstic acid in a 60°C paraffin oven. Demonstration of collagen in the modifications of Masson and fibrin was done with either light green or aniline blue; saffron was used in the Movat pentachrome.All 3 techniques were improved in quality and precision with the aid of heat. Although fibrin was demonstrated in all techniques, minute quantities were better seen in the fibrin stain because the red cells were stained in a different color. These modified stains demonstrated several entities in a single slide preparation in about 20 min. (The J Histotechnol16:349, 1993)

ISSN:0147-8885    

DOI:10.1179/his.1993.16.4.349

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

AbstractThe Holmes method for soft tissue staining of nerve fibers was modified to produce excellent detail of the canalicular network in decalcified bone tissue. With simple changes in timing and the meticulous use of fresh reagents, we were able to stain bone lacunae and canaliculi in a variety of animal and human tissues. This technique produced more uniform and accurate results than the thionin/picric acid stains used previously for visualization of osteocytes. The morphologic results can be used for diagnostic purposes as well as stereological studies where the osteocyte network is of importance. (The J Histotechnol16:355, 1993)

ISSN:0147-8885    

DOI:10.1179/his.1993.16.4.355

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

AbstractA double-bladed scalpel for cutting tissue sections of uniform thickness is described. A scalpel handle was modified to permit the insertion of adjacent parallel blades separated by a 3 mm gap. This instrument enhances the ability to cut tissue sections of a constant thickness before the samples are submitted for embedding. (The J Histotechnol16:363, 1993)

ISSN:0147-8885    

DOI:10.1179/his.1993.16.4.363

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

AbstractSeveral tissue sections of a cervical lymph node diagnosed as lymphoma presented on light microscopy with an unusual nuclear artifact of lymphoid cells after fixation in 10% neutral buffered formalin and staining with hematoxylin and eosin. Nuclei appeared shrunken with homogeneous dispersion of chromatin and spiked at their periphery. Inadequate irnrnersion of tissue sections in one paraffin bath, leaving blocks uncovered, was found to have caused the artifact. When routine care was exercised in replacing, or at least rotating, paraffin baths each time the tissue processor was changed to fresh fixative, dehydrants, and clearing agents, the problem was resolved. (The J Histotechnol16:365, 1993)

ISSN:0147-8885    

DOI:10.1179/his.1993.16.4.365

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

AbstractTwo xylene substitutes (a terpene and an isoparaffinic hydrocarbon) were compared to xylene as the deparaffinization agent in an automated immunostainer. Human kidney tissue was stained for cytokeratin with amino-ethyl carbazole as the chromogen. There was no discernable difference in either staining intensity, background staining, or effectiveness of paraffin removal among the 3 clearing agents. There also were no differences among these parameters when the slides were reviewed after 8 mo storage in the dark, with the exception of the staining intensity, whichdiminishedequally withal1 clearing agents. The occupational, environmental, and regulatory aspects of these 3 clearing agents are discussed. (The J Histotechnol16:367, 1993)

ISSN:0147-8885    

DOI:10.1179/his.1993.16.4.367

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor