ISSN:0147-8885    

DOI:10.1179/his.1977.1.2.38

出版商:Taylor&Francis    

年代:1977

数据来源: Taylor

ISSN:0147-8885    

DOI:10.1179/his.1977.1.2.39

出版商:Taylor&Francis    

年代:1977

数据来源: Taylor

ISSN:0147-8885    

DOI:10.1179/his.1977.1.2.49

出版商:Taylor&Francis    

年代:1977

数据来源: Taylor

摘要:

AbstractDue to the capriciousness of available staining methods for the demonstration of spirochetal organisms in tissue and because of the need for a large volume staining technic, the Dieterle spirochete stain has been modified and used successfully in this laboratory for several years. This method recently gained new importance by successfully demonstrating the etiologic agent of“Legionnaires' disease”in paraffin embedded tissue after attempts made with routine stains for bacteria and fungi were not satisfactory.

ISSN:0147-8885    

DOI:10.1179/his.1977.1.2.51

出版商:Taylor&Francis    

年代:1977

数据来源: Taylor

摘要:

AbstractHepatocyte cytoplasmic inclusions in six biopsy and eight autopsy liver specimens from patients with chronic alcoholism showing unequivocal alcoholic hyalin (Mallory bodies) at the light microscopic level were defined by electron microscopy. Additionally, these structures were found to be generally identifiable in the same and other paraffin embedded liver tissue reprocessed for electron microscopy. The inclusions were ultrastructurally grouped as five nonmembrane limited and two complete or partially membrane bound admixtures of fibrillar, granular and amorphous components of variable distribution and electron density. Membrane free types included characteristic (A) interdigitating filaments, (B) closely packed filamentous and amorphous material, (C) whorl-like fibrils in parallel array, (D) loosely arranged discrete or intertwined long wavy fibrils or membranous fragments, (E) large serpiginous masses of highly electron dense amorphous material. Membrane limited forms showed (F) scattered vesicular and fibrillar or (G) uniformly particulate and fibrillar content, completely or partially surrounded by a single or double membrane respectively.The demonstrated ultrastructural diversity of hepatocyte cytoplasmic inclusions and their stability even after postmortem autolysis of paraffin processing, or both, is of significance in diagnosis as well as in potential analysis of isolated liver cell fractions.

ISSN:0147-8885    

DOI:10.1179/his.1977.1.2.56

出版商:Taylor&Francis    

年代:1977

数据来源: Taylor

摘要:

AbstractMost of the silver impregnation methods used on renal biopsies are very sensitive, time consuming and need constant attention for successful results. The use of the familiar, simple and rapid Wilder's reticulum stain for demonstrating the membrane changes in renal biopsies is described.

ISSN:0147-8885    

DOI:10.1179/his.1977.1.2.63

出版商:Taylor&Francis    

年代:1977

数据来源: Taylor

摘要:

AbstractTraditional methods for the staining of nerve fibers and endings in paraffin sections are tricky and lengthy.1 2Silver nitrate technics often produce precipitates on the sections and. there is a tendency for sections to be lost due to floating free from the slides. Existing copper protargol procedures tend to stain some connective tissues (especially after 10% formalin fixation),3require the use of counterstains, and are of long duration. However, the use of urea4in the staining solution, a slight lowering of pH and the raising of the temperature makes the use of copper protargol practical on a day to day basis by overcoming these difficulties.

ISSN:0147-8885    

DOI:10.1179/his.1977.1.2.65

出版商:Taylor&Francis    

年代:1977

数据来源: Taylor

摘要:

AbstractEmbedding paraffins commonly used for eye microtomy are discussed. A relatively new paraffin product may be used also for eye microtomy and light microscopy preparations of excellent quality.

ISSN:0147-8885    

DOI:10.1179/his.1977.1.2.67

出版商:Taylor&Francis    

年代:1977

数据来源: Taylor

ISSN:0147-8885    

DOI:10.1179/his.1977.1.2.68

出版商:Taylor&Francis    

年代:1977

数据来源: Taylor