ISSN:0147-8885    

DOI:10.1179/his.1986.9.1.6

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

摘要:

AbstractA review of recent literature indicates that histopathologists continue to be worried by problems of interobserver and intraobserver variations in the diagnoses of histological specimens. It is suggested that quantification would go a long way toward solving some of these problems. The basic ideas of stereology are discussed and illustrated by worked examples. Recent applications of stereology to histopathology are given. The problems of sampling and other difficulties are commented upon. A role for the histotechnologist is suggested.

ISSN:0147-8885    

DOI:10.1179/his.1986.9.1.9

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

摘要:

AbstractA simple and affordable immunocytochemical technique at the ultrastructural level using a hydrophilic resin is described in modified form and in detail. In the method, ultrathin sections of tissue embedded in L-R white are imrnunostained in three stages using the versatile avidin-biotinylated horseradish peroxidase sequence and 3,3′-diaminobenzidine. The staining reaction is intense and ultrastructural detail is well preserved.

ISSN:0147-8885    

DOI:10.1179/his.1986.9.1.13

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

摘要:

AbstractDual staining with fluorescein and phycoerythrin conjugated monoclonal antibodies allows the detection of two different lymphocyte populations or determines the expression of two different antigens on a single cell.

ISSN:0147-8885    

DOI:10.1179/his.1986.9.1.15

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

摘要:

AbstractAn economical method for substituting highly diluted radio immunoassay(RIA)antisera for conventional antisera in peroxidase antiperoxidase (PAP) immunohistochemical staining is des cribed. The technique requires material routinely used in the laboratory and permits the use of readily available RIA antisera in place of the more concentrated antibodies available specifically for immunohistochemical staining.

ISSN:0147-8885    

DOI:10.1179/his.1986.9.1.17

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

摘要:

AbstractSpecial tissue culture (histoculture) methodologv, when coibined with hematoxylin and eosin (H&E) stained tissue sections, ultrastructural studies, and time-lapse cinephotomicrography, can provide information in neoplastic tissue that cannot be obtained from H&E alone. This approach has unlimited applicability and is particularly useful inthe evaluation of mitotic cells and host response to the disease process. The multidiciplinary nature of this approach yields results superior to any single method.

ISSN:0147-8885    

DOI:10.1179/his.1986.9.1.21

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

摘要:

AbstractA modification of the sulfation-toluidine blue procedure for the detection of fungi in tissue is described. This method uses a sulfation reagent, which is safer to mix and use than the previous reagent, and a counterstain, which provides an improved contrast between the fungi and the background. This modification method is simple to use, and the results are comparable to the original stain.

ISSN:0147-8885    

DOI:10.1179/his.1986.9.1.23

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

ISSN:0147-8885    

DOI:10.1179/his.1986.9.1.25

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

摘要:

AbstractSome tissues like bone and muscle are adversely affected by conventional xylene clearing. We investigated the potential of methyl salicylate as a substitute clearing agent.

ISSN:0147-8885    

DOI:10.1179/his.1986.9.1.27

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor

ISSN:0147-8885    

DOI:10.1179/his.1986.9.1.29

出版商:Taylor&Francis    

年代:1986

数据来源: Taylor