ISSN:0147-8885    

DOI:10.1179/his.1993.16.1.11

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

AbstractThe immunogold-silver technique was modified so that 1–2μm thick glycolmethacrylate sections could routinely be used for high resolution immunocytochemistry. A rabbit antihuman hemoglobin antibody was used to demonstrate the distribution of exogenous hemoglobin in mouse tissues. Inclusion of 0.1% glutaraldehyde in the primary formalin fixation improved tissue appearance. Glutaraldehyde treatment after colloidal gold antibody application increased the staining intensity, and gold chloride intensification of the silverenhanced colloidal gold further improved immunosensitivity. Thus, a more accurate analysis of hemoglobin distribution in tissue was obtained. The enhancements were also beneficial for localizing myelin basic protein in brain and for determining the proliferative status of tissue by localization of bromodeoxyuridine incorporation into nuclei.Manual capillary action staining efficiency was demonstrated during all steps in the immunogold-silver staining protocol, greatly simplifying the procedure, reducing technician time, and producing better staining consistency. The modified immunogold-silver staining technique is, thus, simple, sensitive, economical, and relatively nontoxic. (The J Histotechnol16:13, 1993)

ISSN:0147-8885    

DOI:10.1179/his.1993.16.1.13

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

AbstractThe characteristics of silver enhancement of immunogold staining at the electron microscopy level were evaluated. Five differently sized gold particles (1,5,15,30 and 40 nm) were tested for 10 different enhancement durations (1–17 min) with atrial natriuretic peptide in rat atria as a model. We found that the size of the particles grew in an accelerated fashion within certain time intervals. Beyond this duration, the variation in particle sizes and shapes increased out ofthe range ofany practical value. A semiquantitative correlation between particle size and the duration of silver treatment was derived. The silver enhancement did not affect the background of the immunostaining, and the silver enhanced 1 nm gold was not as sensitive as the 5 nm gold. An attempt was made to use these properties of silver enhancement in a double immunostaining procedure and we found that its application in this regard was feasible but limited. This study provides a quantitative basis for immunogold silver immunocytochemistry and can be used as a guideline for future applications. (The J Histotechnol16:19, 1993)

ISSN:0147-8885    

DOI:10.1179/his.1993.16.1.19

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

AbstractThe purpose of this study was to determine whether the detection of cytomegalovirus (CMV) in formalin fixed, paraffin embedded tissue is best accomplished by immunohistology or in situ hybridization. Most of the tissues analyzed had the characteristic cellular inclusions. Thus, the detection rate for the mmunohistochemical assay was markedly dependent on the type of proteinase and varied from 25% with trypsin digestion to 90% with proteinase K digestion. All 16 tissues were positive with the in situ hybridization test, and the number of positive cells in serial sections was slightly greater than the immunohistochemical assay with proteinase K digestion. Thus, the detection rates of CMV in fixed tissue sections for in situ hybridization and immunohistochemistry were equivalent if proteinase K digestion was used for the latter technique. (The J Histotechnol16:27, 1993)

ISSN:0147-8885    

DOI:10.1179/his.1993.16.1.27

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

AbstractApplication of a manual capillary action workstation to colorimetric in situ hybridization is described. This system, commercially available as the MicroProbe system, is designed to take advantage of capillary action for the application of reagents, with subsequent reduction in technologist effort, conservation of reagents and reproducibility of results within an assay. The system can accommodate 20 slides, and results are comparable to those obtained with automated technology. Although the robotic system provides even greater freedom for the technologist and the ability to perform 60 slides per assay, the MicroProbe system provides a relatively inexpensive alternative for the introduction of new technology. (The J Histotechnol16:33, 1993)

ISSN:0147-8885    

DOI:10.1179/his.1993.16.1.33

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

AbstractWe report an immunohistochemical method for the assay of estrogen and progesterone receptors (ER/PR) in zinc-sulfate formalin fixed, paraffin embedded tissues of breast carcinomas with Abbott's monoclonal antibodies to ER protein (H222) and PRprotein (JZB39). Small portions of tumor were placed in zinc sulfate formalin, which was then used either as a primary- or a postfixative. Sections were pretreated briefly with trypsin and pronase, and the combination of a supersensitive streptavidin detection system with dilution of the primary monoclonal antibodies yielded an immunoreaction with high nuclear signal and low background. In those instances when only small amounts of breast cancer are available, as is increasingly common, this technique may prove invaluable. (The J Histotechnol16:51, 1993)

ISSN:0147-8885    

DOI:10.1179/his.1993.16.1.51

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

AbstractThe increased use of mammography has resulted in progressively smaller primary breast tumors. This trend has increased the need to perform immunohistochemical estrogen receptor analysis with formalin fixed, paraffin embedded biopsies. Nine laboratories participated in an interlaboratory study to determine the degree of variability of immunostaining results and their interpretation. The results of this pilot study indicate the need for standardization of assay protocols and the need to promote the use of freshly made formalin for optimum results. (The J Histotechnol16:57, 1993)

ISSN:0147-8885    

DOI:10.1179/his.1993.16.1.57

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

AbstractHardness of the blocks of polymerized methylmethacrylate (MMA, a plastic embedding medium widely used in calcified tissue histology) was measured at the top, the bottom, and the sides of the blocks. In addition, the most superficial layers of resins were removed with a grinding machine to explore the hardness in the inner parts of the blocks. A kinetic study of hardness changes was also done in all these areas during a period of 60 days. We observed an altered polymerization at the top of the blocks that we attribute to incompletely excluded atmospheric oxygen. However, a defect in the polymerization process was also observed on the lateral sides and the bottom of the blocks, suggesting complex changes induced by surface contact: MMA continued to harden quickly after blocks were been removed from the molds, and polymerization was not completed even if the blocks looked hard and suitable for sectioning. Heightening the tissue specimen with polyester foam or sponge (to avoid contact with the bottom of the mold) and waiting one or two days after removal from the mold were easy ways to avoid embedding, sectioning and staining artifacts.(The J Histotechnol16:65, 1993)

ISSN:0147-8885    

DOI:10.1179/his.1993.16.1.65

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

AbstractIntracellular crystalline deposits were observed in visceral adipose tissue in nearly one-third of stillborn autopsies reviewed. These spicular brown crystals are a frequent finding in stillbirth autopsies. The artifact, sometimes prominent and extensive, should be recognized as formalin pigment and without pathologic consequence. (The J Histotechnol16:69,1993)

ISSN:0147-8885    

DOI:10.1179/his.1993.16.1.69

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

AbstractA theoretical and historical overview of paraffin wax processing is presented. Processing effects chemical and physical changes in each step of the procedure, therefore appropriate choice of reagents and processing times can minimize microscopic artifacts. Solutions to processing problems are offered. (The J Histotechnol16:71, 1992)

ISSN:0147-8885    

DOI:10.1179/his.1993.16.1.71

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor