ISSN:0147-8885    

DOI:10.1179/his.1994.17.1.7

出版商:Taylor&Francis    

年代:1994

数据来源: Taylor

摘要:

AbstractAutometallography (AMG) is a process by which nanometer sized gold particles and crystal lattices of sulfide or selenide of silver, mercury, or zinc encyst themselves in silver. This technique allows light and electron microscopy tracing of the metals in tissue sections from organisms that have been exposed to salts of silver, gold, or mercury. Because it is the crystal lattices that are silver encapsulated, the technique is extremely precise, but also very sensitive, as only a few atoms of gold or molecules of mercury selenide are necessary to initiate the AMG process. We report detailed protocols for AMG methods for each metal and ways of differentiating between them.The central nervous system contains a unique group of zinc enriched (ZEN) neurons, which enclose a specific pool of chelatable zinc in aportionoftheir synaptic vesicles. This zinc pool can be transformed either to zinc selenide crystal lattices by in vivo treatment with sodium selenide or to zinc sulfide crystal lattices after transcardiac perfusion with sodium sulfide. After AMG development of the crystals, a most detailed pattern created by ZEN terminals can be observed. (The J Histotechnol17:15, 1994)

ISSN:0147-8885    

DOI:10.1179/his.1994.17.1.15

出版商:Taylor&Francis    

年代:1994

数据来源: Taylor

摘要:

AbstractAn automated method for 5-bromo-2′-deoxyuridine (BrdU) staining of mouse lung tissue with the Fisher Code-On series is described. BrdU labeling indices (LI) were statistically analyzed to determine the efficacy of automated vs manual staining to detect BrdU incorporation. In the automated method, which utilized capillary gap technology, tissue-tissue and tissue-non-tissue slide pairing were evaluated. The effects of varying trypsin concentrations and the maximum concentration for producing optimal automated BrdU staining were also determined. Comparisons were made between 10% neutral buffered formalin and modified B5 (mercuric chloride) fixed tissues. The automated and manual BrdU staining methods were comparable in overall quality. Analysis of variance procedures revealed no significant differences between the manual and automated procedures for LI in the treated and control animals. In the automated method, no significant difference in the intensity or pattern of staining was found in the variations of slide pairing. A 0.3 % trypsin concentration enhanced the intensity of nuclear staining compared to 0.006 % trypsin; however, the optimal trypsin concentration in which cellular integrity was maintained was determined to be 0.0375 % . Tissues preserved in modified B5 fixative had distorted cellularmorphology and decreased intensity of staining compared to tissues fixed in 10% neutral buffered formalin, after trypsin digestion. (The J Histotechnol17:23, 1994)

ISSN:0147-8885    

DOI:10.1179/his.1994.17.1.23

出版商:Taylor&Francis    

年代:1994

数据来源: Taylor

摘要:

AbstractTaurine may act as an antioxidant, preventing development of inflammatory response and morphologic alteration in lungs of hamsters exposed to nitrogen dioxide. It may bind to plasma membrane reactive sites or stabilize specific molecules in cells inhibiting destructive processes. Localization of taurine is necessary in confirming its role. Hamsters were injected intraperitoneally with H3labeled taurine (0.1μCi/ml sp act 20 Ci/mmol) and sacrificed 1 hr or 18 hr after injection. Lungs were removed. fixed, and embedded in Epon. Control lungs were preparedto compare in vivo and in vitro labeling. Lung slices from untreated hamsters were incubated for 1 hr in McCoy 5A medium and then in medium containing H3taurine for 1 hr. Slices were washed in cold taurine for 1 hr, fixed, and embedded. One-μm sections were prepared for autoradiographic analyses. In situ and in vitro labeled tissues showed no differences in taurine distribution. All in vitro cells showed reater amounts of label than observed in the in vivo cells. In both cases, highest label concentrations were found in the airway epithelia, type II pneumocytes, and macrophages; to a lesser degree in endothelial cells. The lowest concentrations were found in the type I pneumocy tes. These data indicate that lung cells incorporate exogenous taurine. (The J Histotechnol17:29, 1994)

ISSN:0147-8885    

DOI:10.1179/his.1994.17.1.29

出版商:Taylor&Francis    

年代:1994

数据来源: Taylor

摘要:

AbstractUsing proposed Environmental Protection Agency histologic guidelines for selection of maximum tolerated doses in rodent carcinogenesis bioassays, our pathologists encountered a general problem with assessing the onset of hyperkeratosis and crusting from hematoxylin and eosin (H&E) preparations. Although the H&E stain is commonly used for routine analysis of skin samples for histopathology evaluation, it is usually limited in its capability to distinguish key lesions that are important in dermal bioassays. In seeking to identify a stain to act as an adjunct to the standard H&E method, we screened a series of stains. The best differentiation of early hyperkeratosis and crusting was seen with a modified fourcolor histochemical procedure. This procedure stains keratin a bright yellow and crusting exudates a bright pink to provide an early indicator of skin lesions. This stain is a possible adjunct to the standard H&E in diagnosis of rodent dermal bioassays. (The J Histotechnol17:33, 1994)

ISSN:0147-8885    

DOI:10.1179/his.1994.17.1.33

出版商:Taylor&Francis    

年代:1994

数据来源: Taylor

摘要:

AbstractA rapid (2.25 hr) immunostain for estrogen receptor semiquantitation by image analysis is described. The immunostain utilized Abbott's ER-ICA Primary Antibody and the Code-On robotic irnrnunostainer. The image analysis was done with the Batchelor System Percent Activity Analysis software package utilizing the Optimas image analysis engine and Meyer Instruments equipment.The robotic immunostainer allowed precise control of time and temperature, facilitating reproducibility of the immunostain and semiquantitative measurement of estrogen receptor. The immunostain is an alkaline phosphatase method that is suitable for all specimens, notjust those requiring image analysis semiquantitation. (The J Histotechnol17:47, 1994)

ISSN:0147-8885    

DOI:10.1179/his.1994.17.1.47

出版商:Taylor&Francis    

年代:1994

数据来源: Taylor

摘要:

AbstractThe efficacy of low magnification examination of prostatic tissue was assessed. Tissue obtained bv transurethral resection of prostate from 306 cases was screened for carcinoma with a Zeiss DRC dissecting stereomicroscope at 8×magnification. All the submitted tissue from each patient was processed, with at least 1 section cut from each block. There was complete concordance between dissecting microscope diagnoses and those obtained with a standard light microscope at 25×magnification. Use of the dissecting microscope reduced examination time by approximately 80%.Between 2 and 6 cases of prostatic carcinoma would have been overlooked if various partial sampling protocols had been used in preference to complete sampling of prostatic curettings; therefore the importance of complete sampling of prostatic tissue is discussed. (The J Histotechnol17:51,1994)

ISSN:0147-8885    

DOI:10.1179/his.1994.17.1.51

出版商:Taylor&Francis    

年代:1994

数据来源: Taylor

摘要:

AbstractA rapid procedure for dehydrating and clearing siliceous sponges (Haliclona aquaeductus) and calcified corals (Swiftia exserta) with 2,2 dimethoxypropane and methyl salicylate has been developed. This modification of the Muller and Skinner method offers rapidity, preservation of tissue integrity, reproducibility, and improved staining quality. (The J Histotechnol1755, 1993)

ISSN:0147-8885    

DOI:10.1179/his.1994.17.1.55

出版商:Taylor&Francis    

年代:1994

数据来源: Taylor

摘要:

AbstractThe modified Gomori trichrome, pH 3.4, has been useful in the visualization of the intermyofibrillary network, which is not obvious by connective tissue techniques of the Masson type. However, it is often capricious, requiring fresh preparation before use. The method presented, which uses three stock buffered stain solutions of the required pH, clearly demonstrates both the tissue and cell structure and has been reliably used in the diagnoses of over 600 human muscle biopsies. The solution is easily prepared and remains stable for several months. (The J Histotechnol17:59, 1990)

ISSN:0147-8885    

DOI:10.1179/his.1994.17.1.59

出版商:Taylor&Francis    

年代:1994

数据来源: Taylor

摘要:

AbstractWe describe a classic case of progressive multifocal leu- koencephalopathy (PML) proven by histologic, ultrastruc- tural, and immunocytochemical analysis in a 58 yo female with a history of deteriorating neurologic status and a brain mass observed on magnetic resonance imaging. The presence of JC viral genomes was confirmed by a rapid 70 min automated in situ DNA hybridization assay utilizing either commercially available, nick translated, whole genomic DNA probes or site specific, synthetic 3′multibiotinylated 18 meroligonucleotide probes constructed to hybridize with the JC virus tandem repeat or agnoprotein gene regions. All probes localized JC virus genomes in the nuclei of enlarged, swollen oligodendrocytes and rare bizarre astrocytes, but the oligonucleotide probes showed stronger hybridization signals when the probe concentrations were equalized. The most potent in situ hybridization signals were obtained when a nonsense 15 mer composed of 5 repeating units of the sequence 5′-TAG-3′was added as a spacer at the 3′end of the oligonucleotide probe to extend the 3′multibiotin affinity label. Affinity labeled, synthetic oligonucleotides provide a rational and cost effective approach to molecular neuroviral diagnostics in PML. (The J Histotechnol17:63, 1994)

ISSN:0147-8885    

DOI:10.1179/his.1994.17.1.63

出版商:Taylor&Francis    

年代:1994

数据来源: Taylor