摘要:

AbstractMutant alleles of the RBI locus on human chromosome 13q 14 have previously been linked to the development of retinoblastoma, a tumor of embryonal neural retina. In the bilateral form of the disease, inheritance of a germinal mutation at this locus predisposes each retinal cell to a subsequent second somatic event which results in the formation of the retinoblastoma tumor. Spontaneous unilateral tumor occurrence appears to result from two sequential somatic events. Survivors of the bilateral form of this eye tumor are at substantially increased risk for subsequent development of second primary cancers, particularly osteosarcoma, a bone cancer. Conversely, survivors of unilateral retinoblastoma exhibit the same likelihood of development of osteosarcoma as the general population.In order to determine whether osteosarcoma and retinoblastoma share a common pathogenetic mechanism, we determined restriction fragment length alleles at loci on chromosome 13 in DNA from constitutional tissues of bilaterally affected retinoblastoma patients as well as from their osteosarcoma tumors. These analyses indicated that the loss of constitutional heterozygosity in the tumors occurred specifically for chromosome 13 and appeared to involve the same chromosomal region as that previously identified in retinoblastoma tumors. Similarly, specific loss of constitutional chromosome 13 heterozygosity was also apparent in sporadic osteosarcomas. These results suggest that both diseases are due to the mitotic unmasking of pleitropic recessive mutant alleles at the RBI locus, and that the clinical occurrence of mixed cancer families such as these may be due to the differential expression of a single recessive mutation.

ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1987.tb02233.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1987.tb02234.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

AbstractThe thymic microenvironment involves complex cell interactions among different types of epithelial cells, macrophages, tissue histiocytes and immature and maturing T cells. We describe the isolation of a subset of thymic epithelial cells by selective primary culture followed by SV 40 ori‐and psV‐2 neo co‐transfection. The cloned cells were characterized as subcapsular cortex epithelial cells having hemopoietic growth factor activity, CSF‐1 and LFA3 associated binding sites for T cells. Thus cotransfection with the selectable genes and the gene which gives growth advantage to the transformant enabled us to investigate the rare type of cells which has been difficult to isolate by ordinary tissue culture technique because of mixed cellularity and fibloblast ove

ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1987.tb02235.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1987.tb02236.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

AbstractWe have cloned cDNAs for IgG, induction factor and T cell replacing factor. The recombinant factors were shown to have multiple activities on B cells, which could explain most, if not all, of B cell growth and differentiation factor activities so far claimed. Since the recombinant factors were also shown to affect bone marrow‐derived cells other than B cells, the names of interleukins 4 and 5 were proposed for IgG1induction factor and T cell replacing factor, respectivel

ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1987.tb02237.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

AbstractTwo kinds of human monoclonal antibodies, CLN‐IgG and SLN‐IgG, were obtained from human x human hybridomas, termed CLNH11 and SLNF10, respectively. These antibodies recognized different molecules which were expressed simultaneously on the cell surface of tumor cells. When both antibodies were added into culture medium in the presence of complement, a remarkable synergistic cytotoxic‐effect against glioblastoma cells was obs

ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1987.tb02238.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

AbstractEstablishment of monoclonal antibodies against human germ cell tumors which detected cell surface antigens, 2A2 and 2G10 (subclass; IgM) are described. Distributions of these antigens in human tumors were studied immunohisto‐chemically. They were expressed on immature endodermal components often seen in yolk sac tumors but not on the other solid tumors in childhood. Regarding their specificities on human germ cell tumor, we have established an ELISA system to detect these antigens from patient's sera. In normal and fetal tissue, on the other hand, they showed quite different distributions. In the kidney, for example, 2A2 and 2G10 were present on collecting tubules and proximal/distal tubules respectively which were already observed in fetal kidney, gestational ages 10 weeks. These results indicate the usefulness of these antibodies as the markers for human germ cell tumors and also for somatic cell differentiatio

ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1987.tb02239.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1987.tb02240.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1987.tb02241.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1987.tb02242.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY