摘要:

L‐Asparaginase has long been used in the treatment of acute lymphoblastic leukemia or malignant lymphoma in childhood. To determine cell type specific sensitivity to this drug, the L‐asparaginase‐mediated inhibition of blastogenesis of human peripheral T or B lymphocytes was compared. The rate of incorporation of [3H]‐thymidine into the DNA of either T lymphocytes due to phytohemagglutinin (PHA) or B lymphocytes due toStaphylococcus aureusCowan I (SAC) was measured by the addition ofEscherichia coliL‐asparaginase in the medium. The blastogenic response of either T or B lymphocytes was also determined in medium depleted of exogenous asparagine and/or glutamine, both of which are hydrolyzed by this enzyme. Thein vitroblastogenesis of either human T lymphocytes due to PHA or B lymphocytes due to SAC was inhibited by the inclusion of asparaginase in the medium. The deprivation of exogenous asparagine did not have any inhibitory effect on the blastogenic response of both T and B lymphocytes to each mitogen. On the other hand, the glutamine concentration in the culture medium provided a critical influence on the proliferative response of T and B lymphocytes. The rate of incorporation of [3H]‐thymidine into DNA was increased markedly as the concentration of glutamine was increased from 2‐7‐2 mmol/1. It is concluded that the mechanism of inhibition of PHA‐ or SAC‐stimulated lymphocyte blastogenesis by L‐asparaginase is not asparagine deprivation but glutamine deprivation. Glutamine, which is the most abundant amino acid, is thought to have an important role in the immune re

ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1992.tb01012.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Serum salicylate concentrations were measured in 60 patients with acute phase Kawasaki disease (KD), who were treated with intravenous aspirin (IVASP), to evaluate its antiinflammatory effect in the treatment of KD. Patients with serum salicylate concentrations ± 150μg/ml showed shorter durations of fever (7.1 ± 2.0vs10.4 ± 6.6 days;P<0.05), shorter durations of positive serum C‐reactive protein (14.6 ± 4.5vs22.3 ± 10.6 days;P<0.01) and lower incidences of coronary arterial involvements (0/10vs6/24;P<0.05) than did patients with serum salicylate concentrations<150μg/ml. Significant linear correlations were recognized between daily IVASP dosage and serum salicylate concentrations (r= 0.73;P<0.01), and between serum salicylate concentrations and serum free salicylate concentrations (r= 0.82;P<0.01). These correlations did not differ between the presence and absence of coronary arterial involvements. Based on these findings we concluded that a beneficial anti‐inflammatory effect in the treatment of KD is achieved when the serum salicylate concentration is 150μg/ml, and that such concentrations could be achieved by increasing the daily IVASP dosage to 100 mg/kg per

ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1992.tb01013.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Bronchopulmonary dysplasia (BPD) has become the most common form of chronic lung disease in the neonate. Recently, we have experienced a severe case of BPD and examined the effect of disodium cromoglycate (DSCG) on BPD. The gestational age and birth weight of the patient were 27 weeks and 1,000 g, respectively. Although RDS subsided after surfactant replacement therapy, the arterial‐alveolar oxygen tension ratio (a/APO2) gradually decreased and FiO2increased with age, respectively, and pure oxygen supplementation was eventually required after 67 days of life. The DSCG treatment was commenced at 80 days of life. After 6 days of the inhalation therapy, a/APO2gradually increased. After 10 days of the treatment, the baby was extubated. While the baby was intubated, intratracheal lavage fluid samples were obtained. Eosinophilic cationic protein (ECP) and polymorphonuclear (PMN) elastase concentrations were determined. ECP and PMN elastase concentrations of intratracheal lavage fluids gradually decreased with the DSCG treatment. These results may indicate that DSCG has led to an improvement of pulmonary function and facilitated weaning from mechanical ventilation in an infant with BP

ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1992.tb01014.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

A female child with mosaic partial deletion of 1 lq is reported. At 1 month of age she was presented with congenital glaucoma, trigonocephaly and multiple minor anomalies. She exhibited growth retardation and the typical phenotype of llq‐ syndrome. G‐banding analysis failed to show any abnormality, although subsequent high resolution banding revealed the abnormal karyotype mos 46,XX,del(11)(q23.3 q24.2)/46,XX,del(11)(q23.3 q25). This case is a second case of mosaic llq‐ syndrome and her karyotype suggests that the region of 11q23.3–11q24.2 is critical in 11q‐ syndrome. Congenital glaucoma has never been reported as a complication of llq

ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1992.tb01015.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Peripheral blood stem cells (PBSC) were collected using the Haemonetics V50 from an 8 month old infant weighing 7.8 kg suffering from acute lymphoblastic leukemia in the first complete remission. Leukapheresis was performed according to an exchange transfusion procedure by the two arm method using only a single lumen Broviac catheter. No problem occurred in the patient during this procedure except for a reduction (by half) of the initial platelet count. This method enables one to collect PBSC very safely, even from infants, in a manner that is painless for patients.

ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1992.tb01016.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

We report two children who suffered from sodium bromate intoxication due to ingestion of the second preparation for permanent hair waving (the second permanent preparation). One child suffered from gastrointestinal symptoms only. The other exhibited slight acute renal insufficiency. Results of the histological examination of the kidney in the sick child with acute renal insufficiency showed sporadic epithelial separation of the proximal tubuli under light microscopy. In addition, we could demonstrate more clearly epithelial separation and unbroken tubular basement membranes under electron microscopy (EM). To our knowledge, this is the first report of EM findings in this disease. The mechanism of epithelial injuries by bromate is not clear.

ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1992.tb01017.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

This report describes two cases of neonatal adrenal mass detected antenatally by routine ultrasound (US) examination of pregnant women. Case 1 was recognized by the fetal US at 31 weeks gestation. The mass, located near the right upper pole of the kidney, was echolucent on US examination. A serial US of the mass showed changes of the internal echoes from a cystic lesion to a mixed lesion, and finally to a hyperechogenic lesion due to a neonatal adrenal hemorrhage (NAH). At 33 days, laparotomy was performed, and the pathological finding revealed an NAH owing to the mass bleeding into the adrenal cyst. Case 2 was also detected by fetal US just before birth. The mass of the right upper pole of the kidney was hyperechogenic on US examination. The baby clinically deteriorated after birth because of hypovolemia owing to NAH. A serial US of the mass showed the change from a hyperechogenic to a cystic lesion. Four months later, the mass spontaneously resolved. From the US spectrum, the mass was diagnosed as NAH. These are the second known cases of NAH detected before birth in Japan.

ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1992.tb01018.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1992.tb01019.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Because of the increasing survival rate of extremely low birthweight infants in recent years, the viability limit in the Eugenic Protection Act in Japan was amended from 24 to 22 completed weeks of gestation. The Japan Pediatric Society's survey on the outcome of infants born in 1990 focused on infants born before 24 weeks gestation and less than 500 g. The survival rates of infants born in 23, 22 and before 22 weeks gestation overall at 511 hospitals throughout Japan were 43/118 (36%), 3/36 (8%) and 0/8 (0%), respectively. Of 16 infants, none weighing less than 400 g at birth survived but 16 (12%) of 50 infants between 400 and 499 g survived. Up‐to‐date statistical data is essential to the amendment of the concept of viability and subsequent ethical decision‐making on extremely low birthweight in

ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1992.tb01020.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

The impact of surfactant therapy on chronic lung disease remains uncertain. During the past decade (1982–91), over 300 babies with respiratory distress syndrome (RDS) weighing 501–2,500 g at birth were consecutively treated with surfactant‐TA at our neonatal intensive care unit. Data on 95 RDS babies treated in the first 5 year period (Period 1, 1982–86) were compared with those on 158 RDS babies treated in the second 5 year period (Period 2, 1987–91). Overall respiratory improvement was better in Period 2 than in Period 1. In Period 2, surfactant therapy converted 98% of the babies with moderate/severe RDS to those with ‘near normal’ lung by 72 hr post‐treatment. In Period 2, 95% of the surfactant‐treated babies weighing 501–1,750 g at birth survived, 97% of which required no supplemental oxygen at 40 weeks corrected gestational age. Increased survival rate in the surfactant‐treated babies during the past decade has not been followed by a parallel increase in chronic lung disease. The severity of the initial pulmonary diseaseper sewas not the significant risk factor for chronic lung disease. Several other variables affecting the response to surfactant therapy and outcome have been identified by stepwise logistic regression analysis and include factors related to perinatal events such as birth asphyxia and infection, and other complic

ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1992.tb01021.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY