ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1986.tb00709.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1986.tb00710.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

AbstractThe lysis of leukemic cells by autologous lymphocytes was studied in the common type of acute lymphoblastic leukemia (ALL) in childhood using the 10‐hour51Cr release assay. Peripheral blood lymphocytes (PBL) from common ALL patients lysed autologous leukemic cells (% lysis: 19–45%), when co‐cultivatedin vitrowith cultured non T non B leukemic cell lines (NALM 16, Reh and NALL 1). The effector cells were cytotoxic T lymphocytes (CTL) possessing Leu‐1 and Leu‐2a antigens. The effector CTL were cytotoxic not only for autologous leukemic cells but for cultured non T non B leukemic cell lines, whereas they did not lyse human T cell (CCRF‐CEM), B cell (Daudi) and natural killer cell lines (SPI‐802). Cold target competition assays demonstrated that leukemic cells from childhood common ALL and several non T non B leukemic cell lines shared an antigenic determinant(s) reco

ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1986.tb00711.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

AbstractThe transformation of human B‐lymphocytes by Epstein‐Barr virus (EBV) was studied in microwell culture by determining3H‐thymidine incorporation seven days after infection. B‐lymphocytes were obtained from four human umbilical cord blood samples, four children, and five adults.The level of3H‐thymidine incorporation was highest in the cord blood group and lowest in the children, although the difference between each group was statistically not significant for a wide range of individual variations.Correlation between the rate of surface immunoglobulin (SIgM) positive cells and percentage of EBV‐associated nuclear antigen (EBNA) positive cells 24 hrs after infection of3H‐thymidine incorporation seven days after infection was not significant.Addition of autologous monocytes suppressed the transformation by EB virus in two cases out of four cord blood specimens, and three cases out of five adult specimens, while it enhanced the transformation in one case out of four child specimens. Also, on clonal transformation by EB virus in agar culture, the effect of addition or monocytes was remarkably suppressive in one

ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1986.tb00712.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

AbstractThe rate of clonal transformation of the B‐lymphocyte by Epstein‐Barr virus (EBV) in children was studied in semisolid agar culture. A remarkable influence of the cell donor's age on the rate was found: In the order of age, the rate was highest in cord blood, lower in infants, lowest in young children, somewhat higher in school children and high in adults. The rate of EBV‐associated nuclear antigen (EBNA)‐positive cells 24 h after infection showed little age‐dependent difference. The only significant difference was found between cord blood and young children. From these results, the age‐dependent difference in the rate of clonal transformation seemed to be mainly in the process after EBNA expression through transformed cell colony formation.In the children with trisomy 21 or ataxia‐telangiectasia, who have a high risk of leukemia or malignant lymphoma, the rate of clonal transformation was not higher than in age‐matched

ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1986.tb00713.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

AbstractThyroxine, tri‐iodothyronine, reverse tri‐iodothyronine, free tri‐iodothyronine, free thyroxine, tri‐iodothyronine resin uptake, thyrotropin, thyroxine‐binding globulin and free thyroxine index in both arterial and venous cord blood of 20 normal full‐term newborn infants were investigated. There was little difference in the values in arterial and venous cord blood in all examinations.The results suggest that venous cord blood, which is more easily obtainable in sufficient quantity than arterial cord blood, and arterial cord blood are equally useful in the determination of thyroid function in the new

ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1986.tb00714.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

AbstractChemiluminescent response of whole blood was estimated as a function of acute phase reactant and compared with ESR and CRP in various types of pediatric patients with fever. 0.1 ml of whole blood was used as the material, and two parameters concerning the phagocytic function of whole blood were used: (1) Peak CL; total phagocytic function of whole blood reflecting the number of granulocytes in a specimen and their function; (2) Peak CL per 103whereas viral infections consisting of upper respiratory infection, varicella and systemic bacterial infections showed an increased peak CL, not only due to the increased number of granulocytes, but also due to the enhanced CL response of the granulocytes themselves (thus peak CL per 103PMN was laso increased), whereas viral infections consisting of upper respiratory infection, varicella and mumps, and acute leukemia in the active stage did not have a remarkable influence on the two parameters. In many types of febrile illness peak CL/103PMN was significantly correlated with ESR and CRP, but not with granulocyte counts. ESR and CRP also did not correlate with granulocyte counts. Thus it was confirmed that the whole blood CL method is useful as a quick test with a small amount of specimen for evaluating febrile patients; concerning the intensity of inflammation as well as phagocyte (granulocyte) functions.

ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1986.tb00715.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

AbstractAlong with the advancement of neonatal medicine, “how small is too small?” for the neonate to survive and for the physician to intervene becomes important. On the basis of recent data on very small neonates in Japan, we present our approach to this subject. The overall mortality rate of neonates weighing less than 1,000 gm was 55% and their incidence of major neurological handicaps was 6.6%. There was a clear difference between mortality rates of neonates weighing above and below 700 gm, which were 34% and 80% respectively, but their morbidity rates were more or less the same. The current record of the smallest neonate to survive in Japan is 444 gm. Therefore “how small is too small?” in the 1980's in Japan will be around 700–1,000 gr for generalized socio‐medical agreement for active intervention and around 400–500 gm for individualized decision‐making for po

ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1986.tb00716.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

AbstractSequential CT scans were evaluated in four term infants with severe asphyxia, who subsequently developed neurodevelopmental sequelae. The initial CT scans performed within the first week of life showed poor visualization of the ventricles, diffuse but mild low density in the cerebral hemispheres and the falx image. In two of them, remarkably low density was seen in the basal ganglia and thalami in the second CT scans. The last CT scans demonstrated multicystic encephalomalasia in one case and brain atrophy with thalamic high density due to postichemic hypervascularity in the other. Of the other two cases, one showed low density in the fronto‐temporal region in the initial CT scan and brain atrophy in the sequential scans. The other showed only transient periventricular low density. In conclusion, the morphologic changes seen in sequential CT scans continue until at least two months after birth and neonatal scans alone may not be useful for predicting the outcom

ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1986.tb00717.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

AbstractTwenty‐six children in continuous complete remission from leukemia or lymphoma and no longer receiving chemotherapy were studied to determine the long‐term sequelae in visual, auditory and vestibular function. Ophthalmologic examination revealed cataracts in five patients and an abnormality in the retina in one patient. These abnormalities were not associated with any disturbance of visual acuity. By otorhinolaryngologic examination, slight hearing loss was observed in five patients and an abnoormal righting reflex in one patient. The etiologies of these abnormal findings in visual, auditory and vestibular function were discus

ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1986.tb00718.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY