ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1987.tb00352.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

AbstractSpecific responses toStaphylococcus aureus (S.aureus)were assessed to prove a decrease or defect of specific protection againstS.aureusin patients with hyper‐IgE syndrome (HIES). Interleukin‐1 (IL‐1) production, interleukin‐2 (IL‐2) production and response to IL‐2 were studied in three groups (A —patients with HIES, B — control patients withS.aureusinfections, C — healthy controls). When lymphocytes or monocytes (Mo) were stimulated by lectins, there was no difference in the three groups. In the experiments concerning IL‐1 derived from Mo activated byS.aureus, the proliferation of cultured fibroblasts showed higher levels of DNA synthesis in the A or B group than in the C group. The IL‐2 derived from peripheral blood lymphocytes (PBL) activated byS.aureusshowed higher levels of activity in the B group than in the A or C group. The response to IL‐2 in the A group was decreased as compared with the C group, and a control patient aged 7 years showed the highest level of all subjects. When PBL were activated byS.aureusas a specific antigen, both IL‐2 production and response to IL‐2 showed lower levels of activity in the A group than in the B group.These results indicated that there was a defect or decrease of specific protection againstS.

ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1987.tb00353.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1987.tb00354.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1987.tb00355.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

AbstractWe have shown that cultured basophilic cells, derived from human cord blood cells, release histamine and high molecular weight neutrophil chemotactic activity (HMW‐NCA), in a dose‐dependent manner following calcium ionophore and anti‐IgE challenge. The HMW‐NCA detected from human cultured basophilic cells had a similar molecular weight to that of human chopped lung challenged by anti‐IgE as determined by gel filtration chromatography. These results suggest that cultured basophilic cells might be employed for research into the chemical mediators involved in immediate hyperse

ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1987.tb00356.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1987.tb00357.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

AbstractEosinophils possess membrane receptors for the Fc portions of IgG and IgE and also for fragments of the complement component C3, i.e. C3b (CR1) and C3bi (CR3). A number of mediators of inflammation are known to be able to upregulate eosinophil functionin vitro, including increased expression of membrane receptors.In this study we have utilised a rosette technique to demonstrate the ability of the autacoids PAF‐acether, its inactive precursor lyso‐PAF, leukotriene B4and histamine to enhance eosinophil immunoglobulin receptor expression. Human monoclonal immunoglobulins co‐valently linked to sheep erythrocytes (E) were used as a measure of functional IgGl and IgE receptor expression, while C3b coated E were utilised as a measure of complement receptor expression. Both PAF‐acether, leukotriene B4and to a lesser extent, histamine gave significant increases in eosinophil IgGl and IgE rosette numbers in a time and dose dependent manner. Lyso‐PAF had no such effect. Pre‐incubation of eosinophils with PAF‐acether gave dose‐dependent significant increases in C3b rosetting.These results show that eosinophils can be activatedin vitroby a number of autacoids as measured by functional increases in membrane receptor expression. This may represent a facet ofin vivoeosinophil activation with possible implications for amplification of the inflam

ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1987.tb00358.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1987.tb00359.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1987.tb00360.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

ISSN:1328-8067    

DOI:10.1111/j.1442-200X.1987.tb00361.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY