摘要:

The molecular weight of purified bovine and porcine submaxillary mucins has been investigated by several techniques. Due to the polydispersity of the material, the molecular weight of a given sample has been shown to vary within large limits according to the method used. The apparent molecular weight is also influenced by the experimental conditions such as ionic strength, speed used to reach sedimentation equilibrium, and concentration of the sample.

ISSN:0020-7551    

DOI:10.1111/j.1399-3011.1970.tb01665.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

Normal human gastric juice has been examined for the presence of peptides. Five pooled resting samples have been found to contain a decapeptide of low chromatographic mobility. The sequential structure of this peptide was shown by analysis, and by comparison with a synthetic sample, to beLeu‐Ala‐Ala‐Gly‐Lys‐Val‐Glu‐Asp‐Ser‐AspTwo of the gastric juice samples also contained a peptide of slightly higher Rf value in smaller quantity. This second peptide, though not characterized completely, appeared to have the structure of a nonapeptide. The amino acid composition differed from that of the decapeptide in that leucine was absent; alanine was the N‐terminal amino acid. These peptides have not previously been reported to be present in normal human gastric juice. The authors have not observed any of these peptides in a small number of samples from pernicio

ISSN:0020-7551    

DOI:10.1111/j.1399-3011.1970.tb01666.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

Circular dichroism studies of avian erythrocytes lysine‐rich and serine‐rich histone fractions are reported. Both histones are essentially disordered in aqueous solutions. In aqueous methanol the lysine‐rich fraction undergoes a coil‐to‐helix transition.The same property is enhanced in the serine‐rich histone, and at high methanol percentage the fraction is partiall

ISSN:0020-7551    

DOI:10.1111/j.1399-3011.1970.tb01667.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

ISSN:0020-7551    

DOI:10.1111/j.1399-3011.1970.tb01668.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

Two tryptophan residues were determined in the polypeptide antitumor antibiotic neocarzinostatin using 2‐hydroxy‐5‐nitrobenzyl bromide (HNB) and 2‐nitrophenyl‐sulfenyl chloride (NPS‐Cl) in 50 %

ISSN:0020-7551    

DOI:10.1111/j.1399-3011.1970.tb01669.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

The elucidation of the antigenic structures of human ceruloplasmin, fibrinogen, plasminogen, IgG, α1‐lipoproteins, β1C/A‐, and β1E‐globulins and–in continuation of earlier experiments–also of β‐lipoproteins and of prealbumin by use of the cross‐reactions between human and animal plasmas is reported. The experiments were done employing Ouchterlony's double diffusion technique in the micro‐modification. Absorption controls confirmed these results.The numbers of antigenic determinants with different specificities detected this way are: for ceruloplasmin: 3; for fibrinogen: 3; for plasminogen: 4; for IgG (γ‐chain only): 4; for α‐lipoproteins: 2; for β1C/A‐ and βE‐globulins: 2 each. Relations between the total number of determinants (including also determinants detectable by other methods only) and the chemical structure, especially the number of amino acid residues, are discussed in greater detail. The applicability of the technical approach to other problems of mo

ISSN:0020-7551    

DOI:10.1111/j.1399-3011.1970.tb01670.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

An abnormal human hemoglobin was found in one family living in Nagasaki and was designated Hb‐Nagasaki. It migrated faster than the normal human hemoglobin towards the anode in thin‐layer starch gel electrophoresis with tris‐EDTA‐borate buffer, pH 8.6. The observation suggested that the abnormality occurred in the (S chain. This abnormal ft chain was isolated and digested with trypsin and pepsin, respectively. The abnormal tryptic peptide and the abnormal peptic peptide thus obtained were isolated and purified by high voltage paper electrophoresis and paper chromatography, and the amino acid compositions of these peptides were determined. As a result, it was found that in Hb‐Nagasaki, a glutamic acid was substituted for the lysine residue at β 17 in the normal

ISSN:0020-7551    

DOI:10.1111/j.1399-3011.1970.tb01671.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

Circular dichroism spectra in different media of native, partially reduced, and fully reduced rihonuclease and lysozyme are reported and discussed with reference to the role of the disulfide linkages in maintaining the tertiary structure of the native enzymes. The results obtained indicate a large disorganization of the native molecules when all disulfide bridges are broken even if in both fully reduced proteins there appears to be some residual non‐covalent structure. However, a tendency toward the partial recovery of the native structure is shown by the reduced proteins on decreasing the temperature or in the presence of methanol.In addition it has been found that some particular disulfide linkages are unessential for achieving the overall conformation of the molecule

ISSN:0020-7551    

DOI:10.1111/j.1399-3011.1970.tb01672.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

A trypsin inhibitor, isolated from bovine liver, has been compared to the cat ionic trypsin inhibitor produced by bovine pancreas (Kunitz’inhibitor). The electrophoretic properties and the amino acid compositions of both inhibitors are identical. The tryptic peptides of the inhibitor isolated from liver have been purified by paper chromato‐electrophoresis. The peptide map is similar to that of pancreatic inhibitor and the amino acid compositions of the 9 fragments are virtually the same in both ca

ISSN:0020-7551    

DOI:10.1111/j.1399-3011.1970.tb01673.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

ISSN:0020-7551    

DOI:10.1111/j.1399-3011.1970.tb01674.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY