摘要:

Electrolysis of amino acids and peptides protected on the amino function has been conducted.In aqueous medium the nitro group of w‐nitro arginine and o‐nitro sulfenyl are selectively removed by means of controlled potential electrolysis, whereas phthaloyl, tosyl, formyl, acetoacetyl, xanthyl, benzyloxycarbonyl, t‐butoxycarbonyl are stable under the electrolytic condition emp

ISSN:0020-7551    

DOI:10.1111/j.1399-3011.1971.tb01726.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

ISSN:0020-7551    

DOI:10.1111/j.1399-3011.1971.tb01727.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

摘要:

All component prepared from adult chicken hemoglobin was separated into α and β polypeptide chains by column chromatography. Of the two chains, the a polypeptide chain was digested with trypsin. The soluble tryptic peptides thus obtained were isolated and purified by column chromatography and paper chromatography.On the other hand, the a polypeptic chain in AH component was digested with chymotrypsin. The chymotryptic peptides thus obtained were isolated and purified by column chromatography and paper chromatography the same way as in the case of tryptic peptides. The amino acid compositions of these tryptic and chymotryptic peptides were analyze

ISSN:0020-7551    

DOI:10.1111/j.1399-3011.1971.tb01728.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

摘要:

A synthesis of a lactam analog of actinomycin D is described in which the threonine residues have been replaced byL‐α,β‐diaminopropionic acid. The key reaction, cyclization of the pentapeptide intermediate, was carried by a nitrophenyl ester condensation between the proline and sarcosine residues.[I′,I′‐Bis(L‐α,β‐diaminopropionic acid)]‐actinomycin D exhibited potent ant

ISSN:0020-7551    

DOI:10.1111/j.1399-3011.1971.tb01729.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

摘要:

The total line width at half height (Δvt) of proton magnetic resonances of proteins can be written as the sum of various terms, of which the most important is that due to the dipole‐dipole interaction of adjacent protons (Δ vdd) The contribution of Δ vdd to Δvt is calculated by an equation which includes the correlation time Tcand the inter‐proton distanceb.It is thus possible to explain the experimentally observed absence of C‐2 histidine resonances in a‐chymotrypsin and in some other native proteins and also the absence of S‐CH3 methionine resonances in all the native proteins examined except for a‐lactalbumin. On the other hand, if Δvdd and either Tcorbare known, it should be possible to calculate the inter‐proton distancebor the correlation t

ISSN:0020-7551    

DOI:10.1111/j.1399-3011.1971.tb01730.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY

摘要:

Deoxyribonucleic acid, histone fraction f‐2, and f‐5 were isolated from avian erythrocytes. The DNA‐histone complex was formed by mixing solutions of the two components at high salt molarity and gradually decreasing the salt concentration by dialysis.The binding of histone f‐5 to DNA as revealed by optical rotatory properties does not induce changes in DNA conformation. The moderately lysine‐rich fraction f‐2 on complexing with DNA alters the CD spectrum of the nucleic acid, and the function of this fraction is tentatively proposed to be involved in the supercoiling of

ISSN:0020-7551    

DOI:10.1111/j.1399-3011.1971.tb01731.x

出版商:Blackwell Publishing Ltd    

年代:1971

数据来源: WILEY