摘要:

A new solution hybridization assay was established for the measurement of renin mRNA. The assay makes use of a radioactively labelled renin complementary RNA as hybridization probe and allows rapid and sensitive detection of renin mRNA in amounts as low as 0.5 pg. With this assay it was possible to quantify renin mRNA in tissues with low-gene expression such as brain and heart as well as in testis, kidney and the submandibular gland (SMG) of mice. The concentrations in these organs were 0.03, 0.07, 1, 5.2 and 164 pg renin mRNA/µg RNA, respectively. These results are in agreement with Northern blotting experiments. The general applicability and ease of the solution hybridization assay described here should greatly improve the rapidity of mRNA measurements in future functional studies

ISSN:0263-6352    

出版商:OVID    

年代:1988

数据来源: OVID

摘要:

A controlled trial of the effect of low versus high calcium intake on blood pressure was performed in 15 patients with mild essential hypertension (supine blood pressure after a 1-month run-in period: 145,7 ± 2.6/97.8 ± 0.9 mmHg, mean ± s.e.m)After a 1-week baseline period on a standard calcium intake (900 mg/day, obtained by giving a 500-mg calcium tablet daily, in addition to a 400-mg calcium diet), the patients were randomly entered into a double-blind crossover study of 4-week low calcium intake (400 mg calcium diet plus two placebo tablets/day) and 4-week high calcium intake (1400 mg/day: 400-mg calcium diet plus two 500-mg calcium tablets/day). Compliance with the diets appeared to be satisfactory, based on the results of food record analysisNo significant blood pressure change was observed at the end of the low- compared to the high-calcium regimen. Serum ionized calcium was slightly, but not significantly lower, while 24-h urinary calcium excretion was significantly reduced during the lowcalcium diet. No difference was found in urinary sodium and potassium excretion between the two study periodsWe conclude that moderate modifications of oral calcium intake are not associated with changes in blood pressure within the time span of this study

ISSN:0263-6352    

出版商:OVID    

年代:1988

数据来源: OVID

ISSN:0263-6352    

出版商:OVID    

年代:1988

数据来源: OVID