ISSN:0001-5792    

DOI:10.1159/000207591

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

Mouse bone marrow cells were separated according to their light scattering properties in a light-activated cell sorter. The intensity of the scattered light was measured in a forward direction (FLS) and perpendicular to the incident light (PLS). These two parameters were found to give an indication of cell size and shape. The fractions containing cells with different light scattering properties were assayed for CFU-s (pluripotent stem cells) content and for the incidence of three types of CFU-c (cells committed to granulocyte/macrophage differentiation). These measurements confirm previous size calculations based on sedimentation rate and density separation. During maturation, the different colony-forming units (CFU) show a gradual increase in PLS intensity. This suggests that the transition from CFU-s through to CFU-c 3 is accompanied by a morphological differentiation.

ISSN:0001-5792    

DOI:10.1159/000207592

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

Bone marrow-derived adherent cell layers, containing endothelial cells, fat cells and macrophages will support prolonged haemopoiesis invitro. Evidence suggests that the adherent layer is acting as an invitro haemopoietic inductive environment for stem cell proliferation and differentiation into the variety of committed progenitor cells of the granulocytic, erythroid, megakaryocytic and lymphoid lineages. Using this system we have analysed the factors controlling proliferation of stem cells (CFU-S), differentiation and maturation of granulocyte/macrophage precursor cells (CFU-C), leukaemic transformation by chemicals and viruses, the role of environment versus stem cells in the aetiology of haemopoietic aplasias and the possible usefulness of long-term cultures for bone marrow transplantation.

ISSN:0001-5792    

DOI:10.1159/000207593

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

The kinetic of production of colony-stimulating activity (CSA) inducing mouse and human colony-forming cells (CFU-C) was tested in different human leukocyte culture systems. Stimulated and unstimulated cultures of spleen single cell suspensions, peripheral mononuclear leukocytes and acute monocytic leukemia (AMoL) cells were investigated. With the exception of the AMoL cells, stimulated cultures always revealed higher CSA levels than unstimulated controls. The spleen cell cultures exhibited the highest overall activity showing three molecular species of 70,000, 35,000 and 10,000 daltons activating human CFU-C to form colonies in the agar culture system. Furthermore it could be demonstrated that colony formation could be inhibited by low molecular weight fibrinogen degradation products obtained by digestion of fibrinogen with granulocyte-derived elastase.

ISSN:0001-5792    

DOI:10.1159/000207594

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

Human lymphocytes stimulated with PHA in liquid phase (step 1) and then seeded in a two-layer soft agar system (step 2) grew and developed into T cell colonies. Colony formation was enhanced when the agar culture was supplemented with culture fluid obtained from phytohemagglutinin-treated lymphocytes (Ly-CF). Untreated lymphocytes plated directly in the soft agar system also developed into colonies if the culture medium contained Ly-CF. Mitogen-sensitized T lymphocytes produced a lymphocyte colony enhancing factor in the culture fluid which stimulated lymphocytes into colony formation. The best plating efficiency (1:250) was attained when blood mononuclear cells were seeded. When spleen cell culture fluid or a highly concentrated blood-adherent cell population was added to the soft agar, colony development was strongly inhibited. Monocytes-macrophages secrete a lymphocyte colony inhibiting factor in the culture medium. These lymphokines exert a competitive influence on T cells and thus control and regulate clonal proliferation.

ISSN:0001-5792    

DOI:10.1159/000207595

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

Compared with the common agar culture of hematopoietic colonies in Petri dishes, the use of glass capillary tubes offers a couple of advantages which have been exploited. Among these advantages are less materials, less cells to be seeded, lower costs, easier and quicker colony counting by visual and semi-automated optical scanning, and lower risk of bacterial contamination. In tubes several parameters affecting the colony formation of mouse and human granulocytes and macrophages as well as of human peripheral T and B lymphocyte from mouse spleen were optimized. The applicability of the cultures to assay for inhibiting and stimulating humoral factors and cytostatics was demonstrated.

ISSN:0001-5792    

DOI:10.1159/000207596

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

In the presence of serum, 2-mercaptoethanol-treated bovine serum albumin enhances T cell colony formation as does 2-mercaptoethanol. The factor only partially substitutes for FCS, but neither for the mitogen phytohemagglutinin nor for conditioned medium derived from cultures of adherent cells.

ISSN:0001-5792    

DOI:10.1159/000207597

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

Using acute myeloid leukemia as an example, the concept of abnormal hematopoietic stem cell production has been examined. It is concluded that the major problem does not lie in humoral regulators, either stimulators or inhibitors, of stem cell production but in intrinsic cellular defects which do not allow cells to respond to these materials. The nature of the stem cell defects has not been defined. Similar mechanisms appear to exist in other human hematopoietic disorders in which abnormal stem cell proliferation is a major feature.

ISSN:0001-5792    

DOI:10.1159/000207598

出版商:S. Karger AG    

年代:1979

数据来源: Karger

ISSN:0001-5792    

DOI:10.1159/000207599

出版商:S. Karger AG    

年代:1979

数据来源: Karger

ISSN:0001-5792    

DOI:10.1159/000207600

出版商:S. Karger AG    

年代:1979

数据来源: Karger