摘要:

Haematological and biochemical aspects of a new family with heterozygous Hb·Padova [α30(B11) Glu→Lys] is described. This variant has been found on a screening programme of abnormal haemoglobin in cord blood. It was separated by cellulose acetate, isoelectric focusing, citrate agar, DEAE-cellulose chromatography and by cation exchange high-performance liquid chromatography. The heat stability and the functional properties were found to be normal. This is the third family with Hb·Padova reported, all from the Mediterranean re

ISSN:0001-5792    

DOI:10.1159/000205018

出版商:S. Karger AG    

年代:1990

数据来源: Karger

摘要:

Normal and leukemic hematopoietic cell lysates were labeled with [3H]-diisopropylfluorophosphate ([3H]-DFP), an active site inhibitor of serine hydrolases. The labeled proteins in the lysates Were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by counting of gel segments for radioactivity. The results indicate the presence of distinct [3H]-DFP binding patterns for different normal and leukemic hematopoietic cells; significantly lower labeling in normal or leukemic lymphoid cells compared to myeloid or monocytoid cells; lower labeling in acute myeloblastic leukemia (FAB-M1) as compared to acute myelomonocytic leukemia (FAB-M4), chronic myelomonocytic leukemia or monocytes and an increase in [3H]-DFP binding with cell maturation along granulocytic series. Thus, these patterns could be useful in discriminating acute lymphoblastic leukemia from myeloid/monocytoid types of leukemia and for following maturation of myeloid cells, and perhaps for studying functional or maturation defects in hematopoietic cells in other pathological conditions.

ISSN:0001-5792    

DOI:10.1159/000205019

出版商:S. Karger AG    

年代:1990

数据来源: Karger

摘要:

The effects of uremic serum on the in vitro growth of normal BFU-E and on the burst-promoting activity by normal T-lymphocytes were evaluated separately. The effect of hemodialysis on the removal of possible serum inhibitor(s) was also tested. Sera of 12 uremic patients were shown to provoke a 60% inhibition of the in vitro growth of normal BFU-E and almost complete abolition of burst-promoting activity by T lymphocytes. While hemodialysis significantly removed the inhibition of uremic sera on BFU-E growth, it was rather ineffective in removing the inhibitor(s) of T lymphocytes. The different effects of uremic serum on BFU-E and on T lymphocytes are discussed.

ISSN:0001-5792    

DOI:10.1159/000205020

出版商:S. Karger AG    

年代:1990

数据来源: Karger

摘要:

The theory of ‘homing’ mechanism has been proposed to explain the association of malignant lymphoma involving multiple sites of mucosa-associated lymphoid tissue (MALT). DNA rearrangement in the peripheral blood and bone marrow specimens of 20 patients with MALT lymphoma were studied utilizing JH and Cβ gene probes, aiming to detect circulating lymphoma cells and unrecognized bone marrow involvement. Our search for malignant cells in the peripheral blood was unrewarding and the ‘homing’ theory remained un-proven. However, the study of DNA rearrangement showed to be useful in determining the malignant nature of abnormal lymphoid aggregates found in the bone marrow of these

ISSN:0001-5792    

DOI:10.1159/000205021

出版商:S. Karger AG    

年代:1990

数据来源: Karger

摘要:

We have sought to clarify the definition and importance of newly diagnosed thrombocytopenia in pregnant women by establishing an appropriate reference interval for the platelet count, and by observing the outcome in a cohort of thrombocytopenic pregnant women. The mean platelet count was lower in 2,155 healthy pregnant than non-pregnant women, and varied with race, but did not fall with increasing gestation, except in Black women. 101 of approximately 2,800 women became newly thrombocytopenic during pregnancy by conventional criteria (platelets < 150 × 109/l), without an apparent cause. Using the reference intervals established during the study, however, 24 of these women would not have been regarded as thrombocytopenic. No bleeding tendency was observed in the mothers or their infants. Maternal platelet counts became normal in the postnatal period. Thrombocytopenia occurring for the first time during pregnancy may be a different condition from auto-immune thrombocytopenia, and we suggest the term ‘pregnancy-associated thrombocytopenia’ (PAT). PAT does not appear to be epidemic; it does not threaten the fetus, but it cannot be distinguished from auto-immune thrombocytopenia in individual c

ISSN:0001-5792    

DOI:10.1159/000205022

出版商:S. Karger AG    

年代:1990

数据来源: Karger

摘要:

In contrast to normal human erythroid tissues where the α2:α1-globin mRNA ratio is about 72:28, in human erythroleukemia K562 cells this ratio was found to be quite low, i.e. about 8:92. The ratio was moderately increased by hemin induction and approached almost normal levels after chromosomal transfer from K562 to the mouse erythroleukemia (MEL) cells. We suggest that operationally positive regulatory factors may exist in erythroleukemia cells, modifying the relative α1 and α2-globin gene expression by events following induction and by the adult MEL environment. These factors may act by altering the relative rate of α1 and α2-globin mRNA synt

ISSN:0001-5792    

DOI:10.1159/000205023

出版商:S. Karger AG    

年代:1990

数据来源: Karger

摘要:

We report the development of chronic lymphocytic leukemia in a 48-year-old woman with systemic lupus erythematosus of 5 years’ duration. Although the association of autoimmunity and lymphoreticular malignancy is well known, this particular association has only rarely been reported. Possible mechanisms of pathogenesis are discusse

ISSN:0001-5792    

DOI:10.1159/000205024

出版商:S. Karger AG    

年代:1990

数据来源: Karger

摘要:

We report a case of erythrocytosis in a patient with end-stage renal failure on chronic hemodialysis. The patient with polycystic kidney disease had an average Hb level of 10 g/dl while on hemodialysis for 3 years. He developed erythrocytosis (Hb 17.6 g/dl) following a cadaveric renal transplantation. No signs suggesting polycythemia vera were found. Nonrenal causes of secondary erythrocytosis such as anoxia, hemoglobinopathies or tumors were excluded. Angiography showed renal artery occlusion of the native kidney. Serum erythropoietin level was 85 U/l (normal 52 ± 31 U/l) as measured by 3H-thymidine uptake. It is suggested that ischemia caused by the renal artery thrombosis stimulated the erythropoietin production in the native polycystic kidney

ISSN:0001-5792    

DOI:10.1159/000205025

出版商:S. Karger AG    

年代:1990

数据来源: Karger

摘要:

Drug-induced agranulocytosis is a clinical entity characterized by a selective reduction of circulating neutrophils, usually to a level < 0.2 × 109/1 in relation to the administration of the drug. Quinidine is an antiarrhythmic agent widely used on an outpatient basis with some well-known hematological side effects. Its midterm administration has been related to a few cases of agranulocytosis. Herein, we describe the case of a 60-year-old man with atrial fibrillation who presented quinidine-induced agranulocytosis of abrupt onset only 3 days after the exposure to the drug, recovering normal levels of neutrophils during the 3rd hospitalization day. Pathogenic mechanisms are discussed

ISSN:0001-5792    

DOI:10.1159/000205026

出版商:S. Karger AG    

年代:1990

数据来源: Karger

摘要:

Near-tetraploid chromosomes were observed in a patient with acute myeloblastic leukemia with maturation (M2 in FAB classification). Large and morphologically bizarre leukemic cells and giant neutrophils in each maturation stage were observed in both peripheral blood and bone marrow. Cytogenetic studies revealed that the main stem line was 93; XXYY, +9, and DNA cytofluorometry showed that these large leukemic cells and giant neutrophils had 4C DNA content. These findings strongly suggested that these giant neutrophils were derived from leukemic clone with tetraploidy.

ISSN:0001-5792    

DOI:10.1159/000205027

出版商:S. Karger AG    

年代:1990

数据来源: Karger