ISSN:0001-5792    

DOI:10.1159/000206380

出版商:S. Karger AG    

年代:1984

数据来源: Karger

摘要:

23 patients undergoing marrow transplantation for leukemia or aplastic anemia were given cyclosporin A (CyA) for graft-versus-host disease (GvHD) prophylaxis. A radioimmunoassay was used to monitor CyA serum levels in 13 patients and whole blood levels in 10. Serum creatinine levels were recorded daily until day 30 and then weekly. The severity of acute GvHD was recorded daily for a total of 1,738 patient days. CyA dose was then correlated with CyA serum or blood levels, serum creatinine levels and severity of acute GvHD. The daily dose of administered CyA correlated with serum CyA levels (p = 0.001) but not with whole-blood CyA levels. The cumulative CyA dose correlated with serum creatinine. There was an inverse correlation between the daily CyA dose and the severity of acute GvHD (p = 0.05). On the other hand the total amount of CyA given within 10 days after bone marrow transplantation had no influence on the severity of acute GvHD developing after day 10. Serum and whole-blood levels did not correlate with severity of GvHD nor with creatinine levels. The results of this study point out the nephrotoxicity of CyA, and a low GvHD score with high doses of administered CyA, at least on a daily basis. Serum but not blood levels reflect the dose of CyA given, but are not correlated with nephrotoxicity or GvHD.

ISSN:0001-5792    

DOI:10.1159/000206381

出版商:S. Karger AG    

年代:1984

数据来源: Karger

摘要:

The maturation process of leukemic myelomonocytoid cells derived from peripheral blood of two typical cases of chronic myelomonocytic leukemia was investigated in vitro. In both cases, liquid culture of the leukemic cells in the presence or absence of 12-o-tetradecanoyl phorbol-13-acetate yielded either monocyte-macrophages or mature granulocytes, respectively, when examined by May-Giemsa staining. However, using cytochemical criteria, both of these mature cell fractions were found to be he-terogenous and still contained substantial numbers of cells positive for both nonspecific and chloroacetate esterases. Phagocytic activity was markedly defective in the monocyte-macrophages recovered from culture as well as in freshly-isolated peripheral monocytes in both patients. In addition, active oxygen production from isolated monocytes stimulated by zymosan was also decreased in one of the cases. The results indicate that at least in some cases of chronic myelomonocytic leukemia, leukemic cells have the potential to differentiate in vitro to monocyte-macrophages which, however, show only limited development of functions, as well as freshly-isolated monocytes from peripheral blood.

ISSN:0001-5792    

DOI:10.1159/000206382

出版商:S. Karger AG    

年代:1984

数据来源: Karger

摘要:

The number of bone marrow granulocyte-macrophage progenitor cells (CFU-C) and fibroblastoid colony-forming units (CFU-F) was significantly smaller in acute leukemia patients. To analyze the mechanism by which CFU-C and CFU-F were reduced, the study on murine myeloid leukemia was carried out. In murine leukemia, bone marrow CFU-C and CFU-F decreased in number in correlation with the proliferation of leukemic cells. Murine leukemic cells suppressed the growth of CFU-C and CFU-F in the mixed culture of leukemic cells and normal murine marrow cells. Furthermore, leukemic cell-conditioned medium inhibited the growth of CFU-F. The results show that murine leukemic cells themselves and/or humoral factors produced by them give inhibitory effects on the growth of not only CFU-C but also CFU-F.

ISSN:0001-5792    

DOI:10.1159/000206383

出版商:S. Karger AG    

年代:1984

数据来源: Karger

摘要:

In 53 children with acute lymphoblastic leukaemia (ALL), initial handmirror cell (HMC) count among lymphoblasts was studied in relation to the occurrence of a relapse in the central nervous system (CNS), taking into account the white blood cell count (WBC) and the immunological phenotype. The children were followed for a period limited by (1) the first CNS relapse, (2) death or (3) the closing day of this study. The median follow-up period was 30 months, range 2–106 months. HMC counts were available in bone marrow smears of 41 children and in cytospins of 35 children. Cytospins proved to give more reliable and consistent results than bone marrow smears. In the 35 ‘cytospin’ children no CNS relapses occurred in the group of 16 children with non-T-non-B-ALL and HMC counts above 10%. However, in the group of 10 children with non-T-non-B-ALL and HMC couts below 10%, and in the group of 9 children with T-ALL (HMC ≤ 11 %), 6 and 5, respectively, got a CNS relapse. The CNS relapse-free period was not significantly different between these last two groups, whereas both groups did differ significantly from the group mentioned first (p < 0.01). This was not found in bone marrow smears of 41 children, presumably because of the inaccurate counting results. A low initial HMC count in cytospins is associated with an increased risk for the occurrence of a CNS relapse in children with ALL. This prognostic factor seems to be independent of other prognostic signs such as immunological phenotype and h

ISSN:0001-5792    

DOI:10.1159/000206384

出版商:S. Karger AG    

年代:1984

数据来源: Karger

摘要:

In platelets of subjects affected with myeloproliferative disorders the following lysosomal enzymes were studied: α-mannosidase, α-fucosidase, β-galactosidase, β-glucosidase, β-glucuronidase, β-N-acetylglucosaminidase and acid phosphatase. For each enzyme the specific activity, the optimum of pH and buffer, Km and saturating substrate concentrations, as well as thermostability were determined. Control and patient enzymes showed no diffe

ISSN:0001-5792    

DOI:10.1159/000206385

出版商:S. Karger AG    

年代:1984

数据来源: Karger

摘要:

The antiviral drug ribavirin (1, β-D-ribofuranosyl-1,2,4-triazole-3-carboxamide) produced significant hematologic effects when administered to rhesus monkeys by intramuscular injection over 10 days in doses of 30 or 100 mg/kg/day. The monkeys developed dose-related progressive anemia and thrombocytosis associated with marrow erythroid hypoplasia and megakaryocyte hyperplasia. In addition, bone marrow examination revealed phagocytosis of erythroid elements by histiocytes; vacuolization of erythroid precursors, and to a lesser extent precursors of other cell types; and occasional erythroid precursors with megaloblastoid appearance. The alterations were transient and disappeared on discontinuation of the drug

ISSN:0001-5792    

DOI:10.1159/000206386

出版商:S. Karger AG    

年代:1984

数据来源: Karger

摘要:

A patient with acute monoblastic leukemia (M5A-FAB) with a karyotype 48, XX, +2, +8, t(9;11)(p21;q22) is reported. This new case confirms that the t(9;11) translocation is a characteristic marker in some cases of M5-FAB (French-American-British classification) leukemias.

ISSN:0001-5792    

DOI:10.1159/000206387

出版商:S. Karger AG    

年代:1984

数据来源: Karger

ISSN:0001-5792    

DOI:10.1159/000206388

出版商:S. Karger AG    

年代:1984

数据来源: Karger

摘要:

Superoxide dismutase (SOD) activity was determined in the erythrocytes and leukocytes of 5 patients with Fanconi’s anemia (FA) and 1 with atypical Fanconi’s anemia without any hematological disorder. SOD activity was decreased in the blood cells of the patients with Fanconi’s anemia, but was normal in the atypical patient. The influence of SOD on the chromosome aberrations and hematological disorder in FA is disc

ISSN:0001-5792    

DOI:10.1159/000206389

出版商:S. Karger AG    

年代:1984

数据来源: Karger